TY - JOUR
T1 - Two cell lines from white sturgeon
AU - Hedrick, Ronald
AU - McDowell, Terry S.
AU - Rosemark, Renee
AU - Aronstein, Diane
AU - Lannan, Catharine N.
PY - 1991/1/1
Y1 - 1991/1/1
N2 - Cell lines were established from spleen and heart tissues of a juvenile white sturgeon Acipenser transmontanus and designated WSS-2 and WSH-1, respectively. Isoenzyme analyses of both lines confirmed their host of origin. Both lines were hypotetraploid and possessed micro- and macrochromosomes. The modal chromosome number for the WSS-2 line was 219; the WSH-1 line had a bimodal distribution with modes of 237 and 243. The effects of temperature on growth and susceptibility to selected viral pathogens were examined for the WSS-2 line. Cell replication occurred at 15-30°C, optimum temperature was 25°C. Cell survival was observed after 11 d at 10°C but not at 35°C. The WSS-2 line was refractory to infections with channel catfish virus (CCV) and infectious pancreatic necrosis virus (IPNV) but susceptible to infectious hematopoietic necrosis virus (IHNV) and white sturgeon iridovirus (WSIV). The need for cell lines from the host of origin in studies of viral pathogens of fish was demonstrated by the ability of the WSS-2 line to support replication of WSIV.
AB - Cell lines were established from spleen and heart tissues of a juvenile white sturgeon Acipenser transmontanus and designated WSS-2 and WSH-1, respectively. Isoenzyme analyses of both lines confirmed their host of origin. Both lines were hypotetraploid and possessed micro- and macrochromosomes. The modal chromosome number for the WSS-2 line was 219; the WSH-1 line had a bimodal distribution with modes of 237 and 243. The effects of temperature on growth and susceptibility to selected viral pathogens were examined for the WSS-2 line. Cell replication occurred at 15-30°C, optimum temperature was 25°C. Cell survival was observed after 11 d at 10°C but not at 35°C. The WSS-2 line was refractory to infections with channel catfish virus (CCV) and infectious pancreatic necrosis virus (IPNV) but susceptible to infectious hematopoietic necrosis virus (IHNV) and white sturgeon iridovirus (WSIV). The need for cell lines from the host of origin in studies of viral pathogens of fish was demonstrated by the ability of the WSS-2 line to support replication of WSIV.
UR - http://www.scopus.com/inward/record.url?scp=0001198624&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0001198624&partnerID=8YFLogxK
U2 - 10.1577/1548-8659(1991)120[0528:TCLFWS]2.3.CO;2
DO - 10.1577/1548-8659(1991)120[0528:TCLFWS]2.3.CO;2
M3 - Article
AN - SCOPUS:0001198624
VL - 120
SP - 528
EP - 534
JO - Transactions of the American Fisheries Society
JF - Transactions of the American Fisheries Society
SN - 0002-8487
IS - 4
ER -