Two canine CD1a proteins are differentially expressed in skin

Frank A. Looringh Van Beeck, Dirk M. Zajonc, Peter F Moore, Yvette M. Schlotter, Femke Broere, Victor P M G Rutten, Ton Willemse, Ildiko Van Rhijn

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Lipid antigens are presented to T cells by the CD1 family of proteins. In this study, we characterize the complete dog (Canis familiaris) CD1 locus, which is located on chromosome 38. The canine locus contains eight CD1A genes (canCD1A), of which five are pseudogenes, one canCD1B, one canCD1C, one canCD1D, and one canCD1E gene. In vivo expression of canine CD1 proteins was shown for canCD1a6, canCD1a8, and canCD1b, using a panel of anti-CD1 monoclonal antibodies (mAbs). CanCD1a6 and canCD1a8 are recognized by two distinct mAbs. Furthermore, we show differential transcription of the three canCD1A genes in canine tissues. In canine skin, the transcription level of canCD1A8 was higher than that of canCD1A6, and no transcription of canCD1A2 was detected. Based on protein modeling and protein sequence alignment, we predict that both canine CD1a proteins can bind different glycolipids in their groove. Besides differences in ectodomain structure, we observed the unique presence of three types of cytoplasmic tails encoded by canCD1A genes. cDNA sequencing and expressed sequence tag sequences confirmed the existence of a short, human CD1a-like cytoplasmic tail of four amino acids, of an intermediate length form of 15 amino acids, and of a long form of 31 amino acids.

Original languageEnglish (US)
Pages (from-to)315-324
Number of pages10
JournalImmunogenetics
Volume60
Issue number6
DOIs
StatePublished - Jun 2008

Fingerprint

Canidae
Skin
Genes
Proteins
Amino Acids
Tail
Monoclonal Antibodies
Dogs
Pseudogenes
Sequence Alignment
Glycolipids
Expressed Sequence Tags
Complementary DNA
Chromosomes
T-Lymphocytes
Lipids
Antigens

Keywords

  • Animal models
  • CD1 antigens
  • Skin immunology

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Looringh Van Beeck, F. A., Zajonc, D. M., Moore, P. F., Schlotter, Y. M., Broere, F., Rutten, V. P. M. G., ... Van Rhijn, I. (2008). Two canine CD1a proteins are differentially expressed in skin. Immunogenetics, 60(6), 315-324. https://doi.org/10.1007/s00251-008-0297-z

Two canine CD1a proteins are differentially expressed in skin. / Looringh Van Beeck, Frank A.; Zajonc, Dirk M.; Moore, Peter F; Schlotter, Yvette M.; Broere, Femke; Rutten, Victor P M G; Willemse, Ton; Van Rhijn, Ildiko.

In: Immunogenetics, Vol. 60, No. 6, 06.2008, p. 315-324.

Research output: Contribution to journalArticle

Looringh Van Beeck, FA, Zajonc, DM, Moore, PF, Schlotter, YM, Broere, F, Rutten, VPMG, Willemse, T & Van Rhijn, I 2008, 'Two canine CD1a proteins are differentially expressed in skin', Immunogenetics, vol. 60, no. 6, pp. 315-324. https://doi.org/10.1007/s00251-008-0297-z
Looringh Van Beeck FA, Zajonc DM, Moore PF, Schlotter YM, Broere F, Rutten VPMG et al. Two canine CD1a proteins are differentially expressed in skin. Immunogenetics. 2008 Jun;60(6):315-324. https://doi.org/10.1007/s00251-008-0297-z
Looringh Van Beeck, Frank A. ; Zajonc, Dirk M. ; Moore, Peter F ; Schlotter, Yvette M. ; Broere, Femke ; Rutten, Victor P M G ; Willemse, Ton ; Van Rhijn, Ildiko. / Two canine CD1a proteins are differentially expressed in skin. In: Immunogenetics. 2008 ; Vol. 60, No. 6. pp. 315-324.
@article{5070c4c47f1c4ea290ff42a9b88b6069,
title = "Two canine CD1a proteins are differentially expressed in skin",
abstract = "Lipid antigens are presented to T cells by the CD1 family of proteins. In this study, we characterize the complete dog (Canis familiaris) CD1 locus, which is located on chromosome 38. The canine locus contains eight CD1A genes (canCD1A), of which five are pseudogenes, one canCD1B, one canCD1C, one canCD1D, and one canCD1E gene. In vivo expression of canine CD1 proteins was shown for canCD1a6, canCD1a8, and canCD1b, using a panel of anti-CD1 monoclonal antibodies (mAbs). CanCD1a6 and canCD1a8 are recognized by two distinct mAbs. Furthermore, we show differential transcription of the three canCD1A genes in canine tissues. In canine skin, the transcription level of canCD1A8 was higher than that of canCD1A6, and no transcription of canCD1A2 was detected. Based on protein modeling and protein sequence alignment, we predict that both canine CD1a proteins can bind different glycolipids in their groove. Besides differences in ectodomain structure, we observed the unique presence of three types of cytoplasmic tails encoded by canCD1A genes. cDNA sequencing and expressed sequence tag sequences confirmed the existence of a short, human CD1a-like cytoplasmic tail of four amino acids, of an intermediate length form of 15 amino acids, and of a long form of 31 amino acids.",
keywords = "Animal models, CD1 antigens, Skin immunology",
author = "{Looringh Van Beeck}, {Frank A.} and Zajonc, {Dirk M.} and Moore, {Peter F} and Schlotter, {Yvette M.} and Femke Broere and Rutten, {Victor P M G} and Ton Willemse and {Van Rhijn}, Ildiko",
year = "2008",
month = "6",
doi = "10.1007/s00251-008-0297-z",
language = "English (US)",
volume = "60",
pages = "315--324",
journal = "Immunogenetics",
issn = "0093-7711",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Two canine CD1a proteins are differentially expressed in skin

AU - Looringh Van Beeck, Frank A.

AU - Zajonc, Dirk M.

AU - Moore, Peter F

AU - Schlotter, Yvette M.

AU - Broere, Femke

AU - Rutten, Victor P M G

AU - Willemse, Ton

AU - Van Rhijn, Ildiko

PY - 2008/6

Y1 - 2008/6

N2 - Lipid antigens are presented to T cells by the CD1 family of proteins. In this study, we characterize the complete dog (Canis familiaris) CD1 locus, which is located on chromosome 38. The canine locus contains eight CD1A genes (canCD1A), of which five are pseudogenes, one canCD1B, one canCD1C, one canCD1D, and one canCD1E gene. In vivo expression of canine CD1 proteins was shown for canCD1a6, canCD1a8, and canCD1b, using a panel of anti-CD1 monoclonal antibodies (mAbs). CanCD1a6 and canCD1a8 are recognized by two distinct mAbs. Furthermore, we show differential transcription of the three canCD1A genes in canine tissues. In canine skin, the transcription level of canCD1A8 was higher than that of canCD1A6, and no transcription of canCD1A2 was detected. Based on protein modeling and protein sequence alignment, we predict that both canine CD1a proteins can bind different glycolipids in their groove. Besides differences in ectodomain structure, we observed the unique presence of three types of cytoplasmic tails encoded by canCD1A genes. cDNA sequencing and expressed sequence tag sequences confirmed the existence of a short, human CD1a-like cytoplasmic tail of four amino acids, of an intermediate length form of 15 amino acids, and of a long form of 31 amino acids.

AB - Lipid antigens are presented to T cells by the CD1 family of proteins. In this study, we characterize the complete dog (Canis familiaris) CD1 locus, which is located on chromosome 38. The canine locus contains eight CD1A genes (canCD1A), of which five are pseudogenes, one canCD1B, one canCD1C, one canCD1D, and one canCD1E gene. In vivo expression of canine CD1 proteins was shown for canCD1a6, canCD1a8, and canCD1b, using a panel of anti-CD1 monoclonal antibodies (mAbs). CanCD1a6 and canCD1a8 are recognized by two distinct mAbs. Furthermore, we show differential transcription of the three canCD1A genes in canine tissues. In canine skin, the transcription level of canCD1A8 was higher than that of canCD1A6, and no transcription of canCD1A2 was detected. Based on protein modeling and protein sequence alignment, we predict that both canine CD1a proteins can bind different glycolipids in their groove. Besides differences in ectodomain structure, we observed the unique presence of three types of cytoplasmic tails encoded by canCD1A genes. cDNA sequencing and expressed sequence tag sequences confirmed the existence of a short, human CD1a-like cytoplasmic tail of four amino acids, of an intermediate length form of 15 amino acids, and of a long form of 31 amino acids.

KW - Animal models

KW - CD1 antigens

KW - Skin immunology

UR - http://www.scopus.com/inward/record.url?scp=44449167564&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44449167564&partnerID=8YFLogxK

U2 - 10.1007/s00251-008-0297-z

DO - 10.1007/s00251-008-0297-z

M3 - Article

C2 - 18488214

AN - SCOPUS:44449167564

VL - 60

SP - 315

EP - 324

JO - Immunogenetics

JF - Immunogenetics

SN - 0093-7711

IS - 6

ER -