Abstract
Lipid antigens are presented to T cells by the CD1 family of proteins. In this study, we characterize the complete dog (Canis familiaris) CD1 locus, which is located on chromosome 38. The canine locus contains eight CD1A genes (canCD1A), of which five are pseudogenes, one canCD1B, one canCD1C, one canCD1D, and one canCD1E gene. In vivo expression of canine CD1 proteins was shown for canCD1a6, canCD1a8, and canCD1b, using a panel of anti-CD1 monoclonal antibodies (mAbs). CanCD1a6 and canCD1a8 are recognized by two distinct mAbs. Furthermore, we show differential transcription of the three canCD1A genes in canine tissues. In canine skin, the transcription level of canCD1A8 was higher than that of canCD1A6, and no transcription of canCD1A2 was detected. Based on protein modeling and protein sequence alignment, we predict that both canine CD1a proteins can bind different glycolipids in their groove. Besides differences in ectodomain structure, we observed the unique presence of three types of cytoplasmic tails encoded by canCD1A genes. cDNA sequencing and expressed sequence tag sequences confirmed the existence of a short, human CD1a-like cytoplasmic tail of four amino acids, of an intermediate length form of 15 amino acids, and of a long form of 31 amino acids.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 315-324 |
| Number of pages | 10 |
| Journal | Immunogenetics |
| Volume | 60 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 2008 |
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Keywords
- Animal models
- CD1 antigens
- Skin immunology
ASJC Scopus subject areas
- Immunology
- Genetics
Cite this
Two canine CD1a proteins are differentially expressed in skin. / Looringh Van Beeck, Frank A.; Zajonc, Dirk M.; Moore, Peter F; Schlotter, Yvette M.; Broere, Femke; Rutten, Victor P M G; Willemse, Ton; Van Rhijn, Ildiko.
In: Immunogenetics, Vol. 60, No. 6, 06.2008, p. 315-324.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Two canine CD1a proteins are differentially expressed in skin
AU - Looringh Van Beeck, Frank A.
AU - Zajonc, Dirk M.
AU - Moore, Peter F
AU - Schlotter, Yvette M.
AU - Broere, Femke
AU - Rutten, Victor P M G
AU - Willemse, Ton
AU - Van Rhijn, Ildiko
PY - 2008/6
Y1 - 2008/6
N2 - Lipid antigens are presented to T cells by the CD1 family of proteins. In this study, we characterize the complete dog (Canis familiaris) CD1 locus, which is located on chromosome 38. The canine locus contains eight CD1A genes (canCD1A), of which five are pseudogenes, one canCD1B, one canCD1C, one canCD1D, and one canCD1E gene. In vivo expression of canine CD1 proteins was shown for canCD1a6, canCD1a8, and canCD1b, using a panel of anti-CD1 monoclonal antibodies (mAbs). CanCD1a6 and canCD1a8 are recognized by two distinct mAbs. Furthermore, we show differential transcription of the three canCD1A genes in canine tissues. In canine skin, the transcription level of canCD1A8 was higher than that of canCD1A6, and no transcription of canCD1A2 was detected. Based on protein modeling and protein sequence alignment, we predict that both canine CD1a proteins can bind different glycolipids in their groove. Besides differences in ectodomain structure, we observed the unique presence of three types of cytoplasmic tails encoded by canCD1A genes. cDNA sequencing and expressed sequence tag sequences confirmed the existence of a short, human CD1a-like cytoplasmic tail of four amino acids, of an intermediate length form of 15 amino acids, and of a long form of 31 amino acids.
AB - Lipid antigens are presented to T cells by the CD1 family of proteins. In this study, we characterize the complete dog (Canis familiaris) CD1 locus, which is located on chromosome 38. The canine locus contains eight CD1A genes (canCD1A), of which five are pseudogenes, one canCD1B, one canCD1C, one canCD1D, and one canCD1E gene. In vivo expression of canine CD1 proteins was shown for canCD1a6, canCD1a8, and canCD1b, using a panel of anti-CD1 monoclonal antibodies (mAbs). CanCD1a6 and canCD1a8 are recognized by two distinct mAbs. Furthermore, we show differential transcription of the three canCD1A genes in canine tissues. In canine skin, the transcription level of canCD1A8 was higher than that of canCD1A6, and no transcription of canCD1A2 was detected. Based on protein modeling and protein sequence alignment, we predict that both canine CD1a proteins can bind different glycolipids in their groove. Besides differences in ectodomain structure, we observed the unique presence of three types of cytoplasmic tails encoded by canCD1A genes. cDNA sequencing and expressed sequence tag sequences confirmed the existence of a short, human CD1a-like cytoplasmic tail of four amino acids, of an intermediate length form of 15 amino acids, and of a long form of 31 amino acids.
KW - Animal models
KW - CD1 antigens
KW - Skin immunology
UR - http://www.scopus.com/inward/record.url?scp=44449167564&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=44449167564&partnerID=8YFLogxK
U2 - 10.1007/s00251-008-0297-z
DO - 10.1007/s00251-008-0297-z
M3 - Article
C2 - 18488214
AN - SCOPUS:44449167564
VL - 60
SP - 315
EP - 324
JO - Immunogenetics
JF - Immunogenetics
SN - 0093-7711
IS - 6
ER -