Two Avirulent, lentogenic strains of Newcastle disease virus are cytotoxic for some human pancreatic tumor lines in vitro

Robert J. Walter, Bashar M. Attar, Asad Rafiq, Megan Delimata, Sooraj Lakshmi Tejaswi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Context Pancreatic cancer is the fourth leading cause of cancer death in the U.S. Highly infectious Newcastle disease virus (NDV) strains are known to be very cytotoxic for an array of human tumor cell types in vitro and in vivo but the effects of these and avirulent NDV strains on pancreatic neoplasms are little known. Objective Here, the direct cytolytic effects of the avirulent Hitchner-B1 (B1) and Ulster (U) NDV strains on 7 human pancreatic tumor cell lines and 4 normal human cell lines were studied. Methods Cytotoxicity assays used serially diluted NDV to determine minimum cytotoxic plaque forming unit (PFU) doses. Results For NDV-B1, normal human cells were killed only by relatively high doses (range: 471-3,724 PFU) whereas NDV-U killed these cells at low PFU (range: 0.32-1.60 PFU). Most pancreatic cancer cell types were killed by much lower NDV-B1 doses (range: 0.40-2.60 PFU) while NDV-U killed Capan-1 and SU.86.86 cultures at very low doses (0.00041 PFU and 0.0034 PFU, respectively). Conclusions On average, 1,555 times more NDV-B1 was needed to kill normal cells than most pancreatic tumor cells and 558 times more NDV-U to kill the two most sensitive pancreatic cancer lines. These innately-targeted lentogenic viruses may have meaningful potential in treating pancreatic cancer.

Original languageEnglish (US)
Pages (from-to)502-513
Number of pages12
JournalJournal of the Pancreas
Volume13
Issue number5
StatePublished - Sep 10 2012
Externally publishedYes

Fingerprint

Newcastle disease virus
Pancreatic Neoplasms
Neoplasms
In Vitro Techniques
Tumor Cell Line
Communicable Diseases
Cause of Death
Viruses
Cell Line

Keywords

  • Antineoplastic agents
  • Newcastle disease virus /classification
  • Oncolytic virotherapy
  • Pancreatic neoplasms
  • Viral vaccines /administration and dosage

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Hepatology

Cite this

Two Avirulent, lentogenic strains of Newcastle disease virus are cytotoxic for some human pancreatic tumor lines in vitro. / Walter, Robert J.; Attar, Bashar M.; Rafiq, Asad; Delimata, Megan; Tejaswi, Sooraj Lakshmi.

In: Journal of the Pancreas, Vol. 13, No. 5, 10.09.2012, p. 502-513.

Research output: Contribution to journalArticle

Walter, Robert J. ; Attar, Bashar M. ; Rafiq, Asad ; Delimata, Megan ; Tejaswi, Sooraj Lakshmi. / Two Avirulent, lentogenic strains of Newcastle disease virus are cytotoxic for some human pancreatic tumor lines in vitro. In: Journal of the Pancreas. 2012 ; Vol. 13, No. 5. pp. 502-513.
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N2 - Context Pancreatic cancer is the fourth leading cause of cancer death in the U.S. Highly infectious Newcastle disease virus (NDV) strains are known to be very cytotoxic for an array of human tumor cell types in vitro and in vivo but the effects of these and avirulent NDV strains on pancreatic neoplasms are little known. Objective Here, the direct cytolytic effects of the avirulent Hitchner-B1 (B1) and Ulster (U) NDV strains on 7 human pancreatic tumor cell lines and 4 normal human cell lines were studied. Methods Cytotoxicity assays used serially diluted NDV to determine minimum cytotoxic plaque forming unit (PFU) doses. Results For NDV-B1, normal human cells were killed only by relatively high doses (range: 471-3,724 PFU) whereas NDV-U killed these cells at low PFU (range: 0.32-1.60 PFU). Most pancreatic cancer cell types were killed by much lower NDV-B1 doses (range: 0.40-2.60 PFU) while NDV-U killed Capan-1 and SU.86.86 cultures at very low doses (0.00041 PFU and 0.0034 PFU, respectively). Conclusions On average, 1,555 times more NDV-B1 was needed to kill normal cells than most pancreatic tumor cells and 558 times more NDV-U to kill the two most sensitive pancreatic cancer lines. These innately-targeted lentogenic viruses may have meaningful potential in treating pancreatic cancer.

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