Two alternatively spliced transcripts generated from OsMUS81, a rice homolog of yeast MUS81, are up-regulated by DNA-damaging treatments

Naozumi Mimida; Hiroko Kitamoto; Keishi Osakabe; Marina Nakashima; Yuji Ito; Wolf Dietrich Heyer; Seiichi Toki; Hiroaki Ichikawa

doi:10.1093/pcp/pcm029

Two alternatively spliced transcripts generated from OsMUS81, a rice homolog of yeast MUS81, are up-regulated by DNA-damaging treatments

Naozumi Mimida, Hiroko Kitamoto, Keishi Osakabe, Marina Nakashima, Yuji Ito, Wolf Dietrich Heyer, Seiichi Toki, Hiroaki Ichikawa

Microbiology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

OsMUS81, a rice homolog of the yeast MUS81 endonuclease gene, produced two alternative transcripts, OsMUS81α and OsMUS81β. OsMus81α contained a Helix-hairpin-Helix (HhH) motif at the N- and C-termini, and a conserved XPF-like motif in the center, while the OsMus81β isoform lacked the second HhH motif by alternative splicing of a cryptic intron generating a truncated protein. The two transcripts were induced after DNA-damaging treatments such as high intensity light, UV-C and γ-radiation. The yeast two-hybrid assay detected a strong interaction between OsMus81 and OsRad54 recombinational repair proteins. These findings suggest that OsMus81 functions in maintaining genome integrity through homologous recombination.

Original language	English (US)
Pages (from-to)	648-654
Number of pages	7
Journal	Plant and Cell Physiology
Volume	48
Issue number	4
DOIs	https://doi.org/10.1093/pcp/pcm029
State	Published - Apr 2007

Keywords

Alternative splicing
DNA repair
Homologous recombination
MUS81
Rice
XPF endonuclease superfamily

ASJC Scopus subject areas

Plant Science
Physiology
Cell Biology

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Two alternatively spliced transcripts generated from OsMUS81, a rice homolog of yeast MUS81, are up-regulated by DNA-damaging treatments. / Mimida, Naozumi; Kitamoto, Hiroko; Osakabe, Keishi; Nakashima, Marina; Ito, Yuji; Heyer, Wolf Dietrich; Toki, Seiichi; Ichikawa, Hiroaki.

In: Plant and Cell Physiology, Vol. 48, No. 4, 04.2007, p. 648-654.

Research output: Contribution to journal › Article › peer-review

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title = "Two alternatively spliced transcripts generated from OsMUS81, a rice homolog of yeast MUS81, are up-regulated by DNA-damaging treatments",

abstract = "OsMUS81, a rice homolog of the yeast MUS81 endonuclease gene, produced two alternative transcripts, OsMUS81α and OsMUS81β. OsMus81α contained a Helix-hairpin-Helix (HhH) motif at the N- and C-termini, and a conserved XPF-like motif in the center, while the OsMus81β isoform lacked the second HhH motif by alternative splicing of a cryptic intron generating a truncated protein. The two transcripts were induced after DNA-damaging treatments such as high intensity light, UV-C and γ-radiation. The yeast two-hybrid assay detected a strong interaction between OsMus81 and OsRad54 recombinational repair proteins. These findings suggest that OsMus81 functions in maintaining genome integrity through homologous recombination.",

keywords = "Alternative splicing, DNA repair, Homologous recombination, MUS81, Rice, XPF endonuclease superfamily",

author = "Naozumi Mimida and Hiroko Kitamoto and Keishi Osakabe and Marina Nakashima and Yuji Ito and Heyer, {Wolf Dietrich} and Seiichi Toki and Hiroaki Ichikawa",

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doi = "10.1093/pcp/pcm029",

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AU - Mimida, Naozumi

AU - Kitamoto, Hiroko

AU - Osakabe, Keishi

AU - Nakashima, Marina

AU - Ito, Yuji

AU - Heyer, Wolf Dietrich

AU - Toki, Seiichi

AU - Ichikawa, Hiroaki

PY - 2007/4

Y1 - 2007/4

N2 - OsMUS81, a rice homolog of the yeast MUS81 endonuclease gene, produced two alternative transcripts, OsMUS81α and OsMUS81β. OsMus81α contained a Helix-hairpin-Helix (HhH) motif at the N- and C-termini, and a conserved XPF-like motif in the center, while the OsMus81β isoform lacked the second HhH motif by alternative splicing of a cryptic intron generating a truncated protein. The two transcripts were induced after DNA-damaging treatments such as high intensity light, UV-C and γ-radiation. The yeast two-hybrid assay detected a strong interaction between OsMus81 and OsRad54 recombinational repair proteins. These findings suggest that OsMus81 functions in maintaining genome integrity through homologous recombination.

AB - OsMUS81, a rice homolog of the yeast MUS81 endonuclease gene, produced two alternative transcripts, OsMUS81α and OsMUS81β. OsMus81α contained a Helix-hairpin-Helix (HhH) motif at the N- and C-termini, and a conserved XPF-like motif in the center, while the OsMus81β isoform lacked the second HhH motif by alternative splicing of a cryptic intron generating a truncated protein. The two transcripts were induced after DNA-damaging treatments such as high intensity light, UV-C and γ-radiation. The yeast two-hybrid assay detected a strong interaction between OsMus81 and OsRad54 recombinational repair proteins. These findings suggest that OsMus81 functions in maintaining genome integrity through homologous recombination.

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KW - DNA repair

KW - Homologous recombination

KW - MUS81

KW - Rice

KW - XPF endonuclease superfamily

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JF - Plant and Cell Physiology

SN - 0032-0781

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ER -

Two alternatively spliced transcripts generated from OsMUS81, a rice homolog of yeast MUS81, are up-regulated by DNA-damaging treatments

Abstract

Keywords

ASJC Scopus subject areas

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