Abstract
OsMUS81, a rice homolog of the yeast MUS81 endonuclease gene, produced two alternative transcripts, OsMUS81α and OsMUS81β. OsMus81α contained a Helix-hairpin-Helix (HhH) motif at the N- and C-termini, and a conserved XPF-like motif in the center, while the OsMus81β isoform lacked the second HhH motif by alternative splicing of a cryptic intron generating a truncated protein. The two transcripts were induced after DNA-damaging treatments such as high intensity light, UV-C and γ-radiation. The yeast two-hybrid assay detected a strong interaction between OsMus81 and OsRad54 recombinational repair proteins. These findings suggest that OsMus81 functions in maintaining genome integrity through homologous recombination.
Original language | English (US) |
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Pages (from-to) | 648-654 |
Number of pages | 7 |
Journal | Plant and Cell Physiology |
Volume | 48 |
Issue number | 4 |
DOIs | |
State | Published - Apr 2007 |
Keywords
- Alternative splicing
- DNA repair
- Homologous recombination
- MUS81
- Rice
- XPF endonuclease superfamily
ASJC Scopus subject areas
- Plant Science
- Physiology
- Cell Biology