Abstract
OsMUS81, a rice homolog of the yeast MUS81 endonuclease gene, produced two alternative transcripts, OsMUS81α and OsMUS81β. OsMus81α contained a Helix-hairpin-Helix (HhH) motif at the N- and C-termini, and a conserved XPF-like motif in the center, while the OsMus81β isoform lacked the second HhH motif by alternative splicing of a cryptic intron generating a truncated protein. The two transcripts were induced after DNA-damaging treatments such as high intensity light, UV-C and γ-radiation. The yeast two-hybrid assay detected a strong interaction between OsMus81 and OsRad54 recombinational repair proteins. These findings suggest that OsMus81 functions in maintaining genome integrity through homologous recombination.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 648-654 |
| Number of pages | 7 |
| Journal | Plant and Cell Physiology |
| Volume | 48 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 2007 |
Keywords
- Alternative splicing
- DNA repair
- Homologous recombination
- MUS81
- Rice
- XPF endonuclease superfamily
ASJC Scopus subject areas
- Plant Science
- Physiology
- Cell Biology
Fingerprint Dive into the research topics of 'Two alternatively spliced transcripts generated from OsMUS81, a rice homolog of yeast MUS81, are up-regulated by DNA-damaging treatments'. Together they form a unique fingerprint.
Cite this
Mimida, N., Kitamoto, H., Osakabe, K., Nakashima, M., Ito, Y., Heyer, W. D., Toki, S., & Ichikawa, H. (2007). Two alternatively spliced transcripts generated from OsMUS81, a rice homolog of yeast MUS81, are up-regulated by DNA-damaging treatments. Plant and Cell Physiology, 48(4), 648-654. https://doi.org/10.1093/pcp/pcm029