Two alternatively spliced transcripts generated from OsMUS81, a rice homolog of yeast MUS81, are up-regulated by DNA-damaging treatments

Naozumi Mimida, Hiroko Kitamoto, Keishi Osakabe, Marina Nakashima, Yuji Ito, Wolf Dietrich Heyer, Seiichi Toki, Hiroaki Ichikawa

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

OsMUS81, a rice homolog of the yeast MUS81 endonuclease gene, produced two alternative transcripts, OsMUS81α and OsMUS81β. OsMus81α contained a Helix-hairpin-Helix (HhH) motif at the N- and C-termini, and a conserved XPF-like motif in the center, while the OsMus81β isoform lacked the second HhH motif by alternative splicing of a cryptic intron generating a truncated protein. The two transcripts were induced after DNA-damaging treatments such as high intensity light, UV-C and γ-radiation. The yeast two-hybrid assay detected a strong interaction between OsMus81 and OsRad54 recombinational repair proteins. These findings suggest that OsMus81 functions in maintaining genome integrity through homologous recombination.

Original languageEnglish (US)
Pages (from-to)648-654
Number of pages7
JournalPlant and Cell Physiology
Volume48
Issue number4
DOIs
StatePublished - Apr 2007

Keywords

  • Alternative splicing
  • DNA repair
  • Homologous recombination
  • MUS81
  • Rice
  • XPF endonuclease superfamily

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

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    Mimida, N., Kitamoto, H., Osakabe, K., Nakashima, M., Ito, Y., Heyer, W. D., Toki, S., & Ichikawa, H. (2007). Two alternatively spliced transcripts generated from OsMUS81, a rice homolog of yeast MUS81, are up-regulated by DNA-damaging treatments. Plant and Cell Physiology, 48(4), 648-654. https://doi.org/10.1093/pcp/pcm029