Abstract
Background. Metastasis is the most important predictor of survival in patients with oral squamous cell carcinoma (OSCC). We tested the hypothesis that there is a genetic expression profile associated with OSCC metastasis. Methods. We obtained samples from 6 OSCC node-positive primary tumors and their matched metastatic lymph nodes, and 5 OSCC node-negative primary tumors. Using laser capture microdissection, we isolated OSCC cells from metastatic lymph nodes and compared them with those from matched primary tumors and unmatched node-negative primary tumors using Affymetrix Human Genome Focus arrays. Results. Comparison of tumor cells from the lymph nodes with those from the unmatched, node-negative primary tumors revealed differential expression of 160 genes. Hierarchical clustering and principal component analysis using this 160-gene set showed that the node-negative samples were distinguishable from both, node-positive primary tumors and tumors in the lymph nodes. Many of the expression changes found in the metastatic cells from the lymph nodes were also found in the node-positive primary tumors. Immunohistochemical analysis for transglutaminase-3 and keratin 16 confirmed the differential genetic expression for these genes. Conclusion. These preliminary results suggest that there may be a metastatic gene expression profile present in nodepositive primary OSCC.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 803-814 |
| Number of pages | 12 |
| Journal | Head and Neck |
| Volume | 29 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2007 |
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Keywords
- Genetic profiles
- Lymph nodes
- Metastasis
- Oral cancer
- Oral squamous cell carcinoma
ASJC Scopus subject areas
- Otorhinolaryngology
Cite this
Tumor-specific genetic expression profile of metastatic oral squamous cell carcinoma. / Méndez, Eduardo; Fan, Wenhong; Choi, Peter; Agoff, S. Nicholas; Whipple, Mark; Farwell, D Gregory; Futran, Neal D.; Weymuller, Ernest A.; Zhao, Lue Ping; Chen, Chu.
In: Head and Neck, Vol. 29, No. 9, 09.2007, p. 803-814.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tumor-specific genetic expression profile of metastatic oral squamous cell carcinoma
AU - Méndez, Eduardo
AU - Fan, Wenhong
AU - Choi, Peter
AU - Agoff, S. Nicholas
AU - Whipple, Mark
AU - Farwell, D Gregory
AU - Futran, Neal D.
AU - Weymuller, Ernest A.
AU - Zhao, Lue Ping
AU - Chen, Chu
PY - 2007/9
Y1 - 2007/9
N2 - Background. Metastasis is the most important predictor of survival in patients with oral squamous cell carcinoma (OSCC). We tested the hypothesis that there is a genetic expression profile associated with OSCC metastasis. Methods. We obtained samples from 6 OSCC node-positive primary tumors and their matched metastatic lymph nodes, and 5 OSCC node-negative primary tumors. Using laser capture microdissection, we isolated OSCC cells from metastatic lymph nodes and compared them with those from matched primary tumors and unmatched node-negative primary tumors using Affymetrix Human Genome Focus arrays. Results. Comparison of tumor cells from the lymph nodes with those from the unmatched, node-negative primary tumors revealed differential expression of 160 genes. Hierarchical clustering and principal component analysis using this 160-gene set showed that the node-negative samples were distinguishable from both, node-positive primary tumors and tumors in the lymph nodes. Many of the expression changes found in the metastatic cells from the lymph nodes were also found in the node-positive primary tumors. Immunohistochemical analysis for transglutaminase-3 and keratin 16 confirmed the differential genetic expression for these genes. Conclusion. These preliminary results suggest that there may be a metastatic gene expression profile present in nodepositive primary OSCC.
AB - Background. Metastasis is the most important predictor of survival in patients with oral squamous cell carcinoma (OSCC). We tested the hypothesis that there is a genetic expression profile associated with OSCC metastasis. Methods. We obtained samples from 6 OSCC node-positive primary tumors and their matched metastatic lymph nodes, and 5 OSCC node-negative primary tumors. Using laser capture microdissection, we isolated OSCC cells from metastatic lymph nodes and compared them with those from matched primary tumors and unmatched node-negative primary tumors using Affymetrix Human Genome Focus arrays. Results. Comparison of tumor cells from the lymph nodes with those from the unmatched, node-negative primary tumors revealed differential expression of 160 genes. Hierarchical clustering and principal component analysis using this 160-gene set showed that the node-negative samples were distinguishable from both, node-positive primary tumors and tumors in the lymph nodes. Many of the expression changes found in the metastatic cells from the lymph nodes were also found in the node-positive primary tumors. Immunohistochemical analysis for transglutaminase-3 and keratin 16 confirmed the differential genetic expression for these genes. Conclusion. These preliminary results suggest that there may be a metastatic gene expression profile present in nodepositive primary OSCC.
KW - Genetic profiles
KW - Lymph nodes
KW - Metastasis
KW - Oral cancer
KW - Oral squamous cell carcinoma
UR - http://www.scopus.com/inward/record.url?scp=37349110011&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=37349110011&partnerID=8YFLogxK
U2 - 10.1002/hed.20598
DO - 10.1002/hed.20598
M3 - Article
C2 - 17573689
AN - SCOPUS:37349110011
VL - 29
SP - 803
EP - 814
JO - Head and Neck Surgery
JF - Head and Neck Surgery
SN - 0148-6403
IS - 9
ER -