Tumor-specific genetic expression profile of metastatic oral squamous cell carcinoma

Eduardo Méndez, Wenhong Fan, Peter Choi, S. Nicholas Agoff, Mark Whipple, D Gregory Farwell, Neal D. Futran, Ernest A. Weymuller, Lue Ping Zhao, Chu Chen

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Background. Metastasis is the most important predictor of survival in patients with oral squamous cell carcinoma (OSCC). We tested the hypothesis that there is a genetic expression profile associated with OSCC metastasis. Methods. We obtained samples from 6 OSCC node-positive primary tumors and their matched metastatic lymph nodes, and 5 OSCC node-negative primary tumors. Using laser capture microdissection, we isolated OSCC cells from metastatic lymph nodes and compared them with those from matched primary tumors and unmatched node-negative primary tumors using Affymetrix Human Genome Focus arrays. Results. Comparison of tumor cells from the lymph nodes with those from the unmatched, node-negative primary tumors revealed differential expression of 160 genes. Hierarchical clustering and principal component analysis using this 160-gene set showed that the node-negative samples were distinguishable from both, node-positive primary tumors and tumors in the lymph nodes. Many of the expression changes found in the metastatic cells from the lymph nodes were also found in the node-positive primary tumors. Immunohistochemical analysis for transglutaminase-3 and keratin 16 confirmed the differential genetic expression for these genes. Conclusion. These preliminary results suggest that there may be a metastatic gene expression profile present in nodepositive primary OSCC.

Original languageEnglish (US)
Pages (from-to)803-814
Number of pages12
JournalHead and Neck
Volume29
Issue number9
DOIs
StatePublished - Sep 2007

Keywords

  • Genetic profiles
  • Lymph nodes
  • Metastasis
  • Oral cancer
  • Oral squamous cell carcinoma

ASJC Scopus subject areas

  • Otorhinolaryngology

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