Abstract
Two cytokines important in the regulation of B-cell function are tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). They act at different steps in B-cell differentiation and can be produced by the B cells themselves upon appropriate stimulation. Crosslinking of surface Ig and signaling through CD22 or CD40 lead to increased secretion of both cytokines. Neutralization of TNF-α or IL-6 biologic activity in B-cell cultures results in a significant reduction in B-cell proliferation and Ig secretion. Increased production of these cytokines is found in several diseases associated with aberrant B-cell function. This review will focus on the role of TNF-α and IL-6 in normal and pathophysiological conditions of B-cell function.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 128-132 |
| Number of pages | 5 |
| Journal | Methods: A Companion to Methods in Enzymology |
| Volume | 11 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1997 |
| Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)