Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in B-lymphocyte function

P. Rieckmann, Joseph Tuscano, J. H. Kehrl

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Two cytokines important in the regulation of B-cell function are tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). They act at different steps in B-cell differentiation and can be produced by the B cells themselves upon appropriate stimulation. Crosslinking of surface Ig and signaling through CD22 or CD40 lead to increased secretion of both cytokines. Neutralization of TNF-α or IL-6 biologic activity in B-cell cultures results in a significant reduction in B-cell proliferation and Ig secretion. Increased production of these cytokines is found in several diseases associated with aberrant B-cell function. This review will focus on the role of TNF-α and IL-6 in normal and pathophysiological conditions of B-cell function.

Original languageEnglish (US)
Pages (from-to)128-132
Number of pages5
JournalMethods: A Companion to Methods in Enzymology
Volume11
Issue number1
DOIs
StatePublished - Jan 1997
Externally publishedYes

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Lymphocytes
Interleukin-6
B-Lymphocytes
Tumor Necrosis Factor-alpha
Cells
Cytokines
Cell proliferation
Cell culture
Crosslinking
Cell Differentiation
Cell Culture Techniques
Cell Proliferation

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in B-lymphocyte function. / Rieckmann, P.; Tuscano, Joseph; Kehrl, J. H.

In: Methods: A Companion to Methods in Enzymology, Vol. 11, No. 1, 01.1997, p. 128-132.

Research output: Contribution to journalArticle

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