Abstract
Two cytokines important in the regulation of B-cell function are tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). They act at different steps in B-cell differentiation and can be produced by the B cells themselves upon appropriate stimulation. Crosslinking of surface Ig and signaling through CD22 or CD40 lead to increased secretion of both cytokines. Neutralization of TNF-α or IL-6 biologic activity in B-cell cultures results in a significant reduction in B-cell proliferation and Ig secretion. Increased production of these cytokines is found in several diseases associated with aberrant B-cell function. This review will focus on the role of TNF-α and IL-6 in normal and pathophysiological conditions of B-cell function.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 128-132 |
| Number of pages | 5 |
| Journal | Methods: A Companion to Methods in Enzymology |
| Volume | 11 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
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Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in B-lymphocyte function. / Rieckmann, P.; Tuscano, Joseph; Kehrl, J. H.
In: Methods: A Companion to Methods in Enzymology, Vol. 11, No. 1, 01.1997, p. 128-132.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in B-lymphocyte function
AU - Rieckmann, P.
AU - Tuscano, Joseph
AU - Kehrl, J. H.
PY - 1997/1
Y1 - 1997/1
N2 - Two cytokines important in the regulation of B-cell function are tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). They act at different steps in B-cell differentiation and can be produced by the B cells themselves upon appropriate stimulation. Crosslinking of surface Ig and signaling through CD22 or CD40 lead to increased secretion of both cytokines. Neutralization of TNF-α or IL-6 biologic activity in B-cell cultures results in a significant reduction in B-cell proliferation and Ig secretion. Increased production of these cytokines is found in several diseases associated with aberrant B-cell function. This review will focus on the role of TNF-α and IL-6 in normal and pathophysiological conditions of B-cell function.
AB - Two cytokines important in the regulation of B-cell function are tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). They act at different steps in B-cell differentiation and can be produced by the B cells themselves upon appropriate stimulation. Crosslinking of surface Ig and signaling through CD22 or CD40 lead to increased secretion of both cytokines. Neutralization of TNF-α or IL-6 biologic activity in B-cell cultures results in a significant reduction in B-cell proliferation and Ig secretion. Increased production of these cytokines is found in several diseases associated with aberrant B-cell function. This review will focus on the role of TNF-α and IL-6 in normal and pathophysiological conditions of B-cell function.
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UR - http://www.scopus.com/inward/citedby.url?scp=0030927848&partnerID=8YFLogxK
U2 - 10.1006/meth.1996.0396
DO - 10.1006/meth.1996.0396
M3 - Article
C2 - 8990098
AN - SCOPUS:0030927848
VL - 11
SP - 128
EP - 132
JO - Methods
JF - Methods
SN - 0076-6879
IS - 1
ER -