Tumor necrosis factor-α confers resistance to hypoxic injury in the adult mammalian cardiac myocyte

Masayuki Nakano, Anne A Knowlton, Ziad Dibbs, Douglas L. Mann

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Background - Previous studies in isolated cardiac myocytes have shown that tumor necrosis factor (TNF)-α provokes increased expression of 27- and 70-kD stress proteins as well as manganese superoxide dismutase, suggesting that TNF-α might play a role in mediating stress responses in the heart. Methods and Results - To determine whether TNF-α stimulation would protect isolated cardiac myocytes against environmental stress, myocyte cultures were pretreated with TNF-α for 12 hours and then subjected to continuous hypoxic injury (O2 content, 3 to 5 ppm) for 12 hours, followed by reoxygenation. Cell injury was assessed in terms of lactic dehydrogenase (LDH) release, 45Ca2+ uptake, and MTT metabolism. Pretreatment with TNF-α concentrations ≤50 U/mL significantly attenuated LDH release by hypoxic cells compared with diluent-treated hypoxic cells. Similar findings were observed with respect to 45Ca2+ uptake and MTT metabolism in TNF-α- pretreated cells that were subjected to prolonged hypoxia. To determine the mechanism for the TNF-α-induced protective effect, the cells were pretreated with heat shock protein (HSP) 72 antisense oligonucleotides. These studies showed that the protective effect of TNF-α was not inhibited by antisense oligonucleotides, despite use of a concentration of antisense that was sufficient to attenuate the TNF-α-induced increase in HSP 72 expression. Subsequent studies using mutated TNF ligands showed that activation of both types l and 2 TNF receptors was sufficient to confer a protective response in isolated cardiac myocytes through an as yet unknown pathway(s). Conclusions - Taken together, the above observations demonstrate that TNF-α pretreatment confers resistance to hypoxic stress in the adult cardiac myocyte through a novel mechanism that appears to be different from but not necessarily exclusive of the protective response conferred by HSP 72 expression.

Original languageEnglish (US)
Pages (from-to)1392-1400
Number of pages9
JournalCirculation
Volume97
Issue number14
StatePublished - Apr 14 1998
Externally publishedYes

Fingerprint

Cardiac Myocytes
Tumor Necrosis Factor-alpha
Wounds and Injuries
HSP72 Heat-Shock Proteins
Antisense Oligonucleotides
Oxidoreductases
Milk
Receptors, Tumor Necrosis Factor, Type II
Heat-Shock Proteins
Muscle Cells
Superoxide Dismutase
Ligands

Keywords

  • Genes
  • Hypoxia
  • Myocytes
  • Proteins

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Tumor necrosis factor-α confers resistance to hypoxic injury in the adult mammalian cardiac myocyte. / Nakano, Masayuki; Knowlton, Anne A; Dibbs, Ziad; Mann, Douglas L.

In: Circulation, Vol. 97, No. 14, 14.04.1998, p. 1392-1400.

Research output: Contribution to journalArticle

Nakano, Masayuki ; Knowlton, Anne A ; Dibbs, Ziad ; Mann, Douglas L. / Tumor necrosis factor-α confers resistance to hypoxic injury in the adult mammalian cardiac myocyte. In: Circulation. 1998 ; Vol. 97, No. 14. pp. 1392-1400.
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