Tumor induction by direct injection of cloned v-src DNA into chickens

Y. K T Fung, L. B. Crittenden, A. M. Fadly, Hsing-Jien Kung

Research output: Contribution to journalArticlepeer-review

49 Scopus citations


The in vitro transforming potential of Rous sarcoma viruses has been shown to reside in a gene segment, v-src. To provide direct proof that the isolated v-src sequence contains all the information necessary for sarcoma induction in vivo, we have injected subgenomic and genomic cloned DNA carrying v-src directly into chickens. We report that v-src, without the complements of other viral genes, can effectively induce sarcomas in the host within weeks after injection. The sarcoma tumors generated by subgenomic v-src DNA are of mono- or oligoclonal origin. They have acquired v-src sequences in the genome and expressed high levels of src messages. Intact long terminal repeats do not seem to be essential in the tumorigenic process.

Original languageEnglish (US)
Pages (from-to)353-357
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number2 I
StatePublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • General
  • Genetics


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