Tubulin cofactors and Arl2 are cage-like chaperones that regulate the soluble αβ-tubulin pool for microtubule dynamics

Stanley Nithianantham, Sinh Le, Elbert Seto, Weitao Jia, Julie Leary, Kevin D. Corbett, Jeffrey K. Moore, Jawdat Al-Bassam

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Microtubule dynamics and polarity stem from the polymerization of αβ-tubulin heterodimers. Five conserved tubulin cofactors/chaperones and the Arl2 GTPase regulate α- and β-tubulin assembly into heterodimers and maintain the soluble tubulin pool in the cytoplasm, but their physical mechanisms are unknown. Here, we reconstitute a core tubulin chaperone consisting of tubulin cofactors TBCD, TBCE, and Arl2, and reveal a cage-like structure for regulating αβ-tubulin. Biochemical assays and electron microscopy structures of multiple intermediates show the sequential binding of αβ-tubulin dimer followed by tubulin cofactor TBCC onto this chaperone, forming a ternary complex in which Arl2 GTP hydrolysis is activated to alter αβ-tubulin conformation. A GTPstate locked Arl2 mutant inhibits ternary complex dissociation in vitro and causes severe defects in microtubule dynamics in vivo. Our studies suggest a revised paradigm for tubulin cofactors and Arl2 functions as a catalytic chaperone that regulates soluble αβ-tubulin assembly and maintenance to support microtubule dynamics.

Original languageEnglish (US)
JournaleLife
Volume4
Issue numberJULY2015
DOIs
StatePublished - Jul 24 2015

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Tubulin
Microtubules
GTP Phosphohydrolases
Guanosine Triphosphate
Polymerization
Dimers
Electron microscopy
Conformations
Hydrolysis
Assays
Electron Microscopy
Cytoplasm
Maintenance

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Nithianantham, S., Le, S., Seto, E., Jia, W., Leary, J., Corbett, K. D., ... Al-Bassam, J. (2015). Tubulin cofactors and Arl2 are cage-like chaperones that regulate the soluble αβ-tubulin pool for microtubule dynamics. eLife, 4(JULY2015). https://doi.org/10.7554/eLife.08811.001

Tubulin cofactors and Arl2 are cage-like chaperones that regulate the soluble αβ-tubulin pool for microtubule dynamics. / Nithianantham, Stanley; Le, Sinh; Seto, Elbert; Jia, Weitao; Leary, Julie; Corbett, Kevin D.; Moore, Jeffrey K.; Al-Bassam, Jawdat.

In: eLife, Vol. 4, No. JULY2015, 24.07.2015.

Research output: Contribution to journalArticle

Nithianantham, S, Le, S, Seto, E, Jia, W, Leary, J, Corbett, KD, Moore, JK & Al-Bassam, J 2015, 'Tubulin cofactors and Arl2 are cage-like chaperones that regulate the soluble αβ-tubulin pool for microtubule dynamics', eLife, vol. 4, no. JULY2015. https://doi.org/10.7554/eLife.08811.001
Nithianantham, Stanley ; Le, Sinh ; Seto, Elbert ; Jia, Weitao ; Leary, Julie ; Corbett, Kevin D. ; Moore, Jeffrey K. ; Al-Bassam, Jawdat. / Tubulin cofactors and Arl2 are cage-like chaperones that regulate the soluble αβ-tubulin pool for microtubule dynamics. In: eLife. 2015 ; Vol. 4, No. JULY2015.
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