Tuberculosis is associated with a down-modulatory lung immune response that impairs Th1-type immunity

Alexandre S. Almeida, Patrícia M. Lago, Neio Boechat, Richard C. Huard, Luiz C O Lazzarini, Adalberto R. Santos, Marcelo Nociari, Hongxia Zhu, Beatriz M. Perez-Sweeney, Heejung Bang, Quanhong Ni, Jie Huang, Andrea L. Gibson, Vera C. Flores, Lorena R. Pecanha, Afrânio L. Kritski, José Roberto Lapa E Silva, John L. Ho

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

Immune mediators associated with human tuberculosis (TB) remain poorly defined. This study quantified levels of lung immune mediator gene expression at the time of diagnosis and during anti-TB treatment using cells obtained by induced sputum. Upon comparison to patients with other infectious lung diseases and volunteers, active pulmonary TB cases expressed significantly higher levels of mediators that counteract Th1-type and innate immunity. Despite the concomitant heightened levels of Th1-type mediators, immune activation may be rendered ineffectual by high levels of intracellular (SOCS and IRAK-M) and extracellular (IL-10 and TGF-βRII, IL-1Rn, and IDO) immune suppressive mediators. These modulators are a direct response to Mycobacterium tuberculosis as, by day 30 of anti-TB treatment, many suppressive factors declined to that of controls whereas most Th1-type and innate immune mediators rose above pretreatment levels. Challenge of human immune cells with M. tuberculosis in vitro up-regulated these immune modulators as well. The observed low levels of NO synthase-2 produced by alveolar macrophages at TB diagnosis, along with the heightened amounts of suppressive mediators, support the conclusion that M. tuberculosis actively promotes down-modulatory mediators to counteract Th1-type and innate immunity as an immunopathological strategy. Our data highlight the potential application of immune mediators as surrogate markers for TB diagnosis or treatment response.

Original languageEnglish (US)
Pages (from-to)718-731
Number of pages14
JournalJournal of Immunology
Volume183
Issue number1
DOIs
StatePublished - Jul 1 2009
Externally publishedYes

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Immunity
Tuberculosis
Lung
Mycobacterium tuberculosis
Innate Immunity
Alveolar Macrophages
Sputum
Pulmonary Tuberculosis
Nitric Oxide Synthase
Interleukin-10
Lung Diseases
Communicable Diseases
Volunteers
Therapeutics
Biomarkers
Gene Expression

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Almeida, A. S., Lago, P. M., Boechat, N., Huard, R. C., Lazzarini, L. C. O., Santos, A. R., ... Ho, J. L. (2009). Tuberculosis is associated with a down-modulatory lung immune response that impairs Th1-type immunity. Journal of Immunology, 183(1), 718-731. https://doi.org/10.4049/jimmunol.0801212

Tuberculosis is associated with a down-modulatory lung immune response that impairs Th1-type immunity. / Almeida, Alexandre S.; Lago, Patrícia M.; Boechat, Neio; Huard, Richard C.; Lazzarini, Luiz C O; Santos, Adalberto R.; Nociari, Marcelo; Zhu, Hongxia; Perez-Sweeney, Beatriz M.; Bang, Heejung; Ni, Quanhong; Huang, Jie; Gibson, Andrea L.; Flores, Vera C.; Pecanha, Lorena R.; Kritski, Afrânio L.; Lapa E Silva, José Roberto; Ho, John L.

In: Journal of Immunology, Vol. 183, No. 1, 01.07.2009, p. 718-731.

Research output: Contribution to journalArticle

Almeida, AS, Lago, PM, Boechat, N, Huard, RC, Lazzarini, LCO, Santos, AR, Nociari, M, Zhu, H, Perez-Sweeney, BM, Bang, H, Ni, Q, Huang, J, Gibson, AL, Flores, VC, Pecanha, LR, Kritski, AL, Lapa E Silva, JR & Ho, JL 2009, 'Tuberculosis is associated with a down-modulatory lung immune response that impairs Th1-type immunity', Journal of Immunology, vol. 183, no. 1, pp. 718-731. https://doi.org/10.4049/jimmunol.0801212
Almeida, Alexandre S. ; Lago, Patrícia M. ; Boechat, Neio ; Huard, Richard C. ; Lazzarini, Luiz C O ; Santos, Adalberto R. ; Nociari, Marcelo ; Zhu, Hongxia ; Perez-Sweeney, Beatriz M. ; Bang, Heejung ; Ni, Quanhong ; Huang, Jie ; Gibson, Andrea L. ; Flores, Vera C. ; Pecanha, Lorena R. ; Kritski, Afrânio L. ; Lapa E Silva, José Roberto ; Ho, John L. / Tuberculosis is associated with a down-modulatory lung immune response that impairs Th1-type immunity. In: Journal of Immunology. 2009 ; Vol. 183, No. 1. pp. 718-731.
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