T3 stimulates resting metabolism and UCP-2 and UCP-3 mRNA but not nonphosphorylating mitochondrial respiration in mice

Mika B. Jekabsons, Francine M. Gregoire, Nancy A. Schonfeld-Warden, Craig H Warden, Barbara A Horwitz

Research output: Contribution to journalArticle

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Abstract

The molecular basis for variations in resting metabolic rate (RMR) within a species is unknown. One possibility is that variations in RMR occur because of variations in uncoupling protein 2 (UCP-2) and uncoupling protein 3 (UCP-3) expression, resulting in mitochondrial proton leak differences. We tested the hypothesis that UCP-2 and -3 mRNAs positively correlate with RMR and proton leak. We treated thyroidectomized and sham-operated mice with triiodothyronine (T3) or vehicle and measured RMR, liver, and skeletal muscle mitochondrial nonphosphorylating respiration and UCP-2 and -3 mRNAs. T3 stimulated RMR and liver UCP-2 and gastrocnemius UCP-2 and -3 expression. Mitochondrial respiration was not affected by T3 and did not correlate with UCP-2 and -3 mRNAs. Gastrocnemius UCP-2 and -3 expression did correlate with RMR. We conclude 1) T3 did not influence intrinsic mitochondrial properties such as membrane structure and composition, and 2) variations in UCP-2 and -3 expression may partly explain variations in RMR. One possible explanation for these data is that T3 stimulates the leak in vivo but not in vitro because a posttranslational regulator of UCP-2 and -3 is not retained in the mitochondrial fraction.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume277
Issue number2 40-2
StatePublished - Aug 1999

Fingerprint

Basal Metabolism
Metabolism
Respiration
Messenger RNA
Proteins
Protons
Liver
Uncoupling Protein 2
Uncoupling Protein 3
Membrane structures
Triiodothyronine
Skeletal Muscle
Muscle
Membranes

Keywords

  • Mitochondria
  • Proton leak
  • Thyroid hormone
  • Uncoupling protein

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

T3 stimulates resting metabolism and UCP-2 and UCP-3 mRNA but not nonphosphorylating mitochondrial respiration in mice. / Jekabsons, Mika B.; Gregoire, Francine M.; Schonfeld-Warden, Nancy A.; Warden, Craig H; Horwitz, Barbara A.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 277, No. 2 40-2, 08.1999.

Research output: Contribution to journalArticle

Jekabsons, Mika B. ; Gregoire, Francine M. ; Schonfeld-Warden, Nancy A. ; Warden, Craig H ; Horwitz, Barbara A. / T3 stimulates resting metabolism and UCP-2 and UCP-3 mRNA but not nonphosphorylating mitochondrial respiration in mice. In: American Journal of Physiology - Endocrinology and Metabolism. 1999 ; Vol. 277, No. 2 40-2.
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