Troponin T isoform expression in the normal and failing human left ventricle: a correlation with myofibrillar ATPase activity

P. A W Anderson, N. N. Malouf, A. E. Oakeley, E. D. Pagani, P. D. Allen

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

The expression of troponin T, a thin filament regulatory protein, was examined in normal and failing left ventricles. The samples were obtained from the hearts of patients with severe heart failure who were undergoing cardiac transplantation, and from normal adult hearts that could not be used for transplantation. Western blots of the myofibrillar proteins demonstrated two isoforms, troponin T1 (TnT1) and troponin T2 (TnT2). TnT2 is expressed at significantly higher levels in failing hearts (p<0.004). Western blots of two-dimension SDS-PAGE gels resolved two dominant spots of TnT1 and of TnT2 and several minor troponin T species. Alkaline phosphatase treatment markedly decreased the sizes of the two acidic spots while increasing the two more basic spots by a comparable amount. Myofibrillar ATPase activity had an inverse and negative linear relationship (r=0.7, p<0.02) with the myofibrillar percentage of total troponin T comprised of TnT2. In that heart failure in these transplant patients had multiple bases, we propose that rather than a cause of heart failure, the disease-associated changes in troponin T isoform expression are an adaptation to abnormal myocardial function.

Original languageEnglish (US)
Pages (from-to)117-127
Number of pages11
JournalBasic Research in Cardiology
Volume87
Issue numberSUPPL. 1
StatePublished - 1992
Externally publishedYes

Keywords

  • Adult heart
  • Contractile proteins
  • Heart failure
  • Monoclonal antibody
  • Myocardium
  • Thin filament regulatory proteins

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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    Anderson, P. A. W., Malouf, N. N., Oakeley, A. E., Pagani, E. D., & Allen, P. D. (1992). Troponin T isoform expression in the normal and failing human left ventricle: a correlation with myofibrillar ATPase activity. Basic Research in Cardiology, 87(SUPPL. 1), 117-127.