Tropomyosin is the major mollusk allergen: Reverse transcriptase polymerase chain reaction, expression and IgE reactivity

Ka Hou Chu, Shun Hang Wong, Patrick S Leung

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

The complementary DNAs encoding tropomyosins of the abalone Haliotis diversicolor, the scallop Chlamys nobilis, and the mussel Perna viridis were amplified by reverse transcriptase polymerase chain reaction and thereafter cloned in plasmid vectors for expression. Immunoblot analysis showed that recombinant proteins of abalone, scallop, and mussel tropomyosin were reactive to serum IgE antibodies from subjects allergic to shellfish but not to nonallergic controls. Nucleotide and amino acid sequences of abalone, scallop, and mussel tropomyosins are highly similar (mostly > 55%) to those of crustacean allergens identified as tropomyosins. Absorption experiments showed that recombinant tropomyosins from the 3 mollusks were able to remove serum IgE reactivity against the 38-kDa tropomyosin of the organisms. These results demonstrate that tropomyosin is the major allergen among various common edible mollusks. Yet a comparison between the amino acid sequences of putative epitopes in crustaceans and mollusks suggests that the epitopes in the two groups may be distinct. Parsimony analysis of the nucleotide sequences of tropomyosin from different mollusks suggests that the tropomyosin gene sequence is a useful tool for phylogenetic analysis of this group of animals.

Original languageEnglish (US)
Pages (from-to)499-509
Number of pages11
JournalMarine Biotechnology
Volume2
Issue number5
StatePublished - 2000

Fingerprint

tropomyosins
Tropomyosin
Mollusca
allergens
Reverse Transcriptase Polymerase Chain Reaction
Allergens
Immunoglobulin E
polymerase chain reaction
molluscs
crustacean
serum
amino acid
reverse transcriptase polymerase chain reaction
Pectinidae
parsimony analysis
shellfish
plasmid
antibody
Bivalvia
abalone

Keywords

  • IgE
  • Mollusca
  • Shellfish allergy
  • Tropomyosin

ASJC Scopus subject areas

  • Aquatic Science
  • Biotechnology

Cite this

Tropomyosin is the major mollusk allergen : Reverse transcriptase polymerase chain reaction, expression and IgE reactivity. / Chu, Ka Hou; Wong, Shun Hang; Leung, Patrick S.

In: Marine Biotechnology, Vol. 2, No. 5, 2000, p. 499-509.

Research output: Contribution to journalArticle

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