Tropomodulin 1 regulation of actin is required for the formation of large paddle protrusions between mature lens fiber cells

Catherine Cheng, Roberta B. Nowak, Sondip K. Biswas, Woo Kuen Lo, Paul G FitzGerald, Velia M. Fowler

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

PURPOSE. To elucidate the proteins required for specialized small interlocking protrusions and large paddle domains at lens fiber cell tricellular junctions (vertices), we developed a novel method to immunostain single lens fibers and studied changes in cell morphology due to loss of tropomodulin 1 (Tmod1), an F-actin pointed end–capping protein. METHODS. We investigated F-actin and F-actin–binding protein localization in interdigitations of Tmod1+/+ and Tmod1‒/‒single mature lens fibers. RESULTS. F-actin–rich small protrusions and large paddles were present along cell vertices of Tmod1+/+ mature fibers. In contrast, Tmod1‒/‒ mature fiber cells lack normal paddle domains, while small protrusions were unaffected. In Tmod1+/+ mature fibers, Tmod1, β2-spectrin, and a-actinin are localized in large puncta in valleys between paddles; but in Tmod1‒/‒ mature fibers, β2-spectrin was dispersed while α-actinin was redistributed at the base of small protrusions and rudimentary paddles. Fimbrin and Arp3 (actin-related protein 3) were located in puncta at the base of small protrusions, while N-cadherin and ezrin outlined the cell membrane in both Tmod1+/+ and Tmod1‒/‒ mature fibers. CONCLUSIONS. These results suggest that distinct F-actin organizations are present in small protrusions versus large paddles. Formation and/or maintenance of large paddle domains depends on a β2-spectrin–actin network stabilized by Tmod1. α-Actinin–crosslinked F-actin bundles are enhanced in absence of Tmod1, indicating altered cytoskeleton organization. Formation of small protrusions is likely facilitated by Arp3-branched and fimbrin-bundled Factin networks, which do not depend on Tmod1. This is the first work to reveal the F-actin– associated proteins required for the formation of paddles between lens fibers.

Original languageEnglish (US)
Article number16
Pages (from-to)4084-4099
Number of pages16
JournalInvestigative Ophthalmology and Visual Science
Volume57
Issue number10
DOIs
StatePublished - Aug 1 2016

Fingerprint

Tropomodulin
Lenses
Actins
Actin-Related Protein 3
Actinin
Spectrin
Proteins
Intercellular Junctions

Keywords

  • Actinin
  • Eye
  • Interdigitations
  • Paddles
  • Spectrin

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Tropomodulin 1 regulation of actin is required for the formation of large paddle protrusions between mature lens fiber cells. / Cheng, Catherine; Nowak, Roberta B.; Biswas, Sondip K.; Lo, Woo Kuen; FitzGerald, Paul G; Fowler, Velia M.

In: Investigative Ophthalmology and Visual Science, Vol. 57, No. 10, 16, 01.08.2016, p. 4084-4099.

Research output: Contribution to journalArticle

Cheng, Catherine ; Nowak, Roberta B. ; Biswas, Sondip K. ; Lo, Woo Kuen ; FitzGerald, Paul G ; Fowler, Velia M. / Tropomodulin 1 regulation of actin is required for the formation of large paddle protrusions between mature lens fiber cells. In: Investigative Ophthalmology and Visual Science. 2016 ; Vol. 57, No. 10. pp. 4084-4099.
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abstract = "PURPOSE. To elucidate the proteins required for specialized small interlocking protrusions and large paddle domains at lens fiber cell tricellular junctions (vertices), we developed a novel method to immunostain single lens fibers and studied changes in cell morphology due to loss of tropomodulin 1 (Tmod1), an F-actin pointed end–capping protein. METHODS. We investigated F-actin and F-actin–binding protein localization in interdigitations of Tmod1+/+ and Tmod1‒/‒single mature lens fibers. RESULTS. F-actin–rich small protrusions and large paddles were present along cell vertices of Tmod1+/+ mature fibers. In contrast, Tmod1‒/‒ mature fiber cells lack normal paddle domains, while small protrusions were unaffected. In Tmod1+/+ mature fibers, Tmod1, β2-spectrin, and a-actinin are localized in large puncta in valleys between paddles; but in Tmod1‒/‒ mature fibers, β2-spectrin was dispersed while α-actinin was redistributed at the base of small protrusions and rudimentary paddles. Fimbrin and Arp3 (actin-related protein 3) were located in puncta at the base of small protrusions, while N-cadherin and ezrin outlined the cell membrane in both Tmod1+/+ and Tmod1‒/‒ mature fibers. CONCLUSIONS. These results suggest that distinct F-actin organizations are present in small protrusions versus large paddles. Formation and/or maintenance of large paddle domains depends on a β2-spectrin–actin network stabilized by Tmod1. α-Actinin–crosslinked F-actin bundles are enhanced in absence of Tmod1, indicating altered cytoskeleton organization. Formation of small protrusions is likely facilitated by Arp3-branched and fimbrin-bundled Factin networks, which do not depend on Tmod1. This is the first work to reveal the F-actin– associated proteins required for the formation of paddles between lens fibers.",
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T1 - Tropomodulin 1 regulation of actin is required for the formation of large paddle protrusions between mature lens fiber cells

AU - Cheng, Catherine

AU - Nowak, Roberta B.

AU - Biswas, Sondip K.

AU - Lo, Woo Kuen

AU - FitzGerald, Paul G

AU - Fowler, Velia M.

PY - 2016/8/1

Y1 - 2016/8/1

N2 - PURPOSE. To elucidate the proteins required for specialized small interlocking protrusions and large paddle domains at lens fiber cell tricellular junctions (vertices), we developed a novel method to immunostain single lens fibers and studied changes in cell morphology due to loss of tropomodulin 1 (Tmod1), an F-actin pointed end–capping protein. METHODS. We investigated F-actin and F-actin–binding protein localization in interdigitations of Tmod1+/+ and Tmod1‒/‒single mature lens fibers. RESULTS. F-actin–rich small protrusions and large paddles were present along cell vertices of Tmod1+/+ mature fibers. In contrast, Tmod1‒/‒ mature fiber cells lack normal paddle domains, while small protrusions were unaffected. In Tmod1+/+ mature fibers, Tmod1, β2-spectrin, and a-actinin are localized in large puncta in valleys between paddles; but in Tmod1‒/‒ mature fibers, β2-spectrin was dispersed while α-actinin was redistributed at the base of small protrusions and rudimentary paddles. Fimbrin and Arp3 (actin-related protein 3) were located in puncta at the base of small protrusions, while N-cadherin and ezrin outlined the cell membrane in both Tmod1+/+ and Tmod1‒/‒ mature fibers. CONCLUSIONS. These results suggest that distinct F-actin organizations are present in small protrusions versus large paddles. Formation and/or maintenance of large paddle domains depends on a β2-spectrin–actin network stabilized by Tmod1. α-Actinin–crosslinked F-actin bundles are enhanced in absence of Tmod1, indicating altered cytoskeleton organization. Formation of small protrusions is likely facilitated by Arp3-branched and fimbrin-bundled Factin networks, which do not depend on Tmod1. This is the first work to reveal the F-actin– associated proteins required for the formation of paddles between lens fibers.

AB - PURPOSE. To elucidate the proteins required for specialized small interlocking protrusions and large paddle domains at lens fiber cell tricellular junctions (vertices), we developed a novel method to immunostain single lens fibers and studied changes in cell morphology due to loss of tropomodulin 1 (Tmod1), an F-actin pointed end–capping protein. METHODS. We investigated F-actin and F-actin–binding protein localization in interdigitations of Tmod1+/+ and Tmod1‒/‒single mature lens fibers. RESULTS. F-actin–rich small protrusions and large paddles were present along cell vertices of Tmod1+/+ mature fibers. In contrast, Tmod1‒/‒ mature fiber cells lack normal paddle domains, while small protrusions were unaffected. In Tmod1+/+ mature fibers, Tmod1, β2-spectrin, and a-actinin are localized in large puncta in valleys between paddles; but in Tmod1‒/‒ mature fibers, β2-spectrin was dispersed while α-actinin was redistributed at the base of small protrusions and rudimentary paddles. Fimbrin and Arp3 (actin-related protein 3) were located in puncta at the base of small protrusions, while N-cadherin and ezrin outlined the cell membrane in both Tmod1+/+ and Tmod1‒/‒ mature fibers. CONCLUSIONS. These results suggest that distinct F-actin organizations are present in small protrusions versus large paddles. Formation and/or maintenance of large paddle domains depends on a β2-spectrin–actin network stabilized by Tmod1. α-Actinin–crosslinked F-actin bundles are enhanced in absence of Tmod1, indicating altered cytoskeleton organization. Formation of small protrusions is likely facilitated by Arp3-branched and fimbrin-bundled Factin networks, which do not depend on Tmod1. This is the first work to reveal the F-actin– associated proteins required for the formation of paddles between lens fibers.

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KW - Eye

KW - Interdigitations

KW - Paddles

KW - Spectrin

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