Tropism of border disease virus for oligodendrocytes in ovine fetal brain cell cultures.

C. A. Anderson, Robert Higgins, A. S. Waldvogel, Bennie Osburn

Research output: Contribution to journalArticle

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Abstract

Primary dissociated ovine brain cell cultures prepared from 50- or 140-day-old fetuses were inoculated with border disease virus (BDV). The cells present in the cultures were identified, using immunofluorescence procedures and sera against various CNS cell-specific markers. These markers were glial fibrillary acidic protein, myelin basic protein, myelin-associated glycoprotein, neuron-specific enolase, neurofilament protein, and fibronectin. Double-labeling immunofluorescence techniques for visualization of BDV antigen and of the CNS cell markers were used to evaluate the pattern of individual cell susceptibility 48 hours after infection. In cultures from fetuses of both ages, about half of the infected cells were glial fibrillary acidic protein-positive astrocytes. Scattered myelin-associated glycoprotein-positive oligodendrocytes were positive for BDV antigen, but only in the infected cultures from the older fetuses. Fibronectin-positive cells were not infected with BDV. In infected and noninfected cultures, cells positive for neuron-specific enolase, myelin basic protein, or neurofilament protein were not seen. Therefore, the remaining infected cells in all the cultures were not identified by the cell-specific markers used. Results of these in vitro experiments indicate that BDV does not selectively infect oligodendrocytes, and that such a selective pattern of infection may not be the basis for the in vivo congenital hypomyelination in sheep with border disease.

Original languageEnglish (US)
Pages (from-to)822-827
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume48
Issue number5
StatePublished - May 1 1987
Externally publishedYes

Fingerprint

Border disease virus
tropisms
Tropism
Oligodendroglia
Sheep
cell culture
Cell Culture Techniques
brain
sheep
Brain
fetus
Myelin-Associated Glycoprotein
cells
phosphopyruvate hydratase
Neurofilament Proteins
fibronectins
myelin sheath
neuroglia
Fetus
Myelin Basic Protein

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Tropism of border disease virus for oligodendrocytes in ovine fetal brain cell cultures. / Anderson, C. A.; Higgins, Robert; Waldvogel, A. S.; Osburn, Bennie.

In: American Journal of Veterinary Research, Vol. 48, No. 5, 01.05.1987, p. 822-827.

Research output: Contribution to journalArticle

Anderson, C. A. ; Higgins, Robert ; Waldvogel, A. S. ; Osburn, Bennie. / Tropism of border disease virus for oligodendrocytes in ovine fetal brain cell cultures. In: American Journal of Veterinary Research. 1987 ; Vol. 48, No. 5. pp. 822-827.
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