Trophic factor supplemented UW solution reduces intimal hyperplasia in the rat aortic transplant model

Chad W. Schmiedt, Michelle C. Schwab, Richard R. Dubielzig, Christopher J Murphy, Jonathan F. McAnulty

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Background: Chronic allograft nephropathy (CAN) is associated with delayed graft function, cold ischemic injury, and is an important cause of premature graft loss. A characteristic vascular lesion of CAN is intimal hyperplasia (IH). The goal of this study was to evaluate the effect of cold storage in University of Wisconsin solution supplemented with trophic factors (UW-TF) on IH in rat aortic isograft (IG) and allograft (AG) models. Methods: F344 → F344 and Lewis → F344 orthotopic abdominal aortic transplants were performed after 48 h of cold storage in either UW or UW-TF solution with and without immunosuppression. Results: Significant reduction in IH was observed when IG were stored in UW-TF solution compared to UW solution. A significant reduction in intimal inflammation was observed in UW-TF stored, nonimmunosuppressed AG. In immunosuppressed recipients, AG stored in UW-TF solution evidenced significantly less IH compared to those stored in UW alone. Conclusions: UW-TF solution decreased IH in both alloindependent and dependent models.

Original languageEnglish (US)
Pages (from-to)204-211
Number of pages8
JournalCryobiology
Volume54
Issue number2
DOIs
StatePublished - Apr 2007
Externally publishedYes

Keywords

  • Intimal hyperplasia
  • Rat aortic transplant model
  • Trophic factors
  • UW solution

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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