Triglyceride-rich lipoprotein lipolysis releases neutral and oxidized FFAs that induce endothelial cell inflammation

Limin Wang, Rajan Gill, Theresa L. Pedersen, Laura J. Higgins, John W. Newman, John C Rutledge

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Triglyceride-rich lipoprotein (TGRL) lipolysis products provide a pro-inflammatory stimulus that can alter endothelial barrier function. To probe the mechanism of this lipolysis-induced event, we evaluated the pro-inflammatory potential of lipid classes derived from human postprandial TGRL by lipoprotein lipase (LpL). Incubation of TGRL with LpL for 30 min increased the saturated and unsaturated FFA content of the incubation solutions significantly. Furthermore, concentrations of the hydroxylated linoleates 9-hydroxy ocatadecadienoic acid (9-HODE) and 13-HODE were elevated by LpL lipolysis,more than other measured oxylipids. The FFA fractions elicited pro-inflammatory responses inducing TNFα and intracellular adhesion molecule expression and reactive oxygen species (ROS) production in human aortic endothelial cells (HAECs). The FFA-mediated increase in ROS was blocked by both the cytochrome P450 2C9 inhibitor sulfaphenazole and NADPH oxidase inhibitors. Compared with linoleate, 13-HODE was found to be a more potent inducer of ROS production in HAECs, an activity that was insensitive to both NADPH oxidase and cytochrome P450 inhibitors. Therefore, although the oxidative metabolism of FFA in endothelial cells can produce inflammatory responses, TGRL lipolysis can also release preformed mediators of oxidative stress (e.g., HODEs) that may influence endothelial cell function in vivo by stimulating intracellular ROS production.

Original languageEnglish (US)
Pages (from-to)204-213
Number of pages10
JournalJournal of Lipid Research
Volume50
Issue number2
DOIs
StatePublished - 2009

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Lipolysis
Endothelial cells
Lipoproteins
Lipoprotein Lipase
Reactive Oxygen Species
Triglycerides
Endothelial Cells
Inflammation
NADPH Oxidase
Cytochrome P-450 Enzyme System
Sulfaphenazole
Hydroxy Acids
Oxidative stress
Linoleic Acid
Metabolism
Oxidative Stress
Adhesion
Lipids
Molecules
13-hydroxy-9,11-octadecadienoic acid

Keywords

  • Endothelial dysfunction
  • Fatty acids
  • Oxidized lipids

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

Cite this

Triglyceride-rich lipoprotein lipolysis releases neutral and oxidized FFAs that induce endothelial cell inflammation. / Wang, Limin; Gill, Rajan; Pedersen, Theresa L.; Higgins, Laura J.; Newman, John W.; Rutledge, John C.

In: Journal of Lipid Research, Vol. 50, No. 2, 2009, p. 204-213.

Research output: Contribution to journalArticle

Wang, Limin ; Gill, Rajan ; Pedersen, Theresa L. ; Higgins, Laura J. ; Newman, John W. ; Rutledge, John C. / Triglyceride-rich lipoprotein lipolysis releases neutral and oxidized FFAs that induce endothelial cell inflammation. In: Journal of Lipid Research. 2009 ; Vol. 50, No. 2. pp. 204-213.
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