Triglyceride-rich lipoprotein lipolysis increases aggregation of endothelial cell membrane microdomains and produces reactive oxygen species

Limin Wang, Annapoorna R. Sapuri-Butti, Hnin Hnin Aung, Atul N. Parikh, John C Rutledge

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Triglyceride-rich lipoprotein (TGRL) lipolysis may provide a proinflammatory stimulus to endothelium. Detergent-resistant plasma membrane microdomains (lipid rafts) have a number of functions in endothelial cell inflammation. The mechanisms of TGRL lipolysis-induced endothelial cell injury were investigated by examining endothelial cell lipid rafts and production of reactive oxygen species (ROS). Lipid raft microdomains in human aortic endothelial cells were visualized by confocal microscopy with fluorescein isothiocyanate-labeled cholera toxin B as a lipid raft marker. Incubation of Atto565-labeled TGRL with lipid raft-labeled endothelial cells showed that TGRL colocalized with the lipid rafts, TGRL lipolysis caused clustering and aggregation of lipid rafts, and colocalization of TGRL remnant particles on the endothelial cells aggregated lipid rafts. Furthermore, TGRL lipolysis caused translocation of low-density lipoprotein receptor-related protein, endothelial nitric oxide synthase, and caveolin-1 from raft regions to nonraft regions of the membrane 3 h after treatment with TGRL lipolysis. TGRL lipolysis significantly increased the production of ROS in endothelial cells, and both NADPH oxidase and cytochrome P-450 inhibitors reduced production of ROS. Our studies suggest that alteration of lipid raft morphology and composition and ROS production could contribute to TGRL lipolysis-mediated endothelial cell injury.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume295
Issue number1
DOIs
StatePublished - Jul 2008

Fingerprint

Membrane Microdomains
Lipolysis
Lipoproteins
Reactive Oxygen Species
Triglycerides
Endothelial Cells
Cell Membrane
Lipids
LDL-Receptor Related Proteins
Caveolin 1
LDL Receptors
Nitric Oxide Synthase Type III
NADPH Oxidase
Cholera Toxin
Wounds and Injuries
Membrane Lipids
Fluorescein
Confocal Microscopy
Detergents
Endothelium

Keywords

  • Endothelial dysfunction
  • Free fatty acids
  • Oxidative stress

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Triglyceride-rich lipoprotein lipolysis increases aggregation of endothelial cell membrane microdomains and produces reactive oxygen species. / Wang, Limin; Sapuri-Butti, Annapoorna R.; Aung, Hnin Hnin; Parikh, Atul N.; Rutledge, John C.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 295, No. 1, 07.2008.

Research output: Contribution to journalArticle

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