Treatment with the Neurosteroid Dehydroepiandrosterone Promotes Recovery of Motor Behavior after Moderate Contusive Spinal Cord Injury in the Mouse

Christelle Fiore, Denise M. Inman, Shinjiro Hirose, Linda J. Noble, Takuji Igarashi, Natalie A. Compagnone

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The neurosteroid dehydroepiandrosterone (DHEA) has neuroprotective properties after ischemic and excitatory insults to the brain. In the developing embryo, it is produced in discrete regions of the central nervous system (CNS), where it specifically promotes axonal growth of differentiated neurons. To test if DHEA could be beneficial after spinal cord injury (SCI), we used a model of moderate contusive SCI developed and characterized in the mouse. Immediately after surgery, we applied treatment with DHEA or with vehicle only and compared treatment groups (n = 12 in each group) over a 42-day period. Locomotor recovery was assessed in an open field using a standardized 21-point scale, according to gait analysis on paw print recordings and using foot fault analyses on an inclined ladder beam. The DHEA-treated group showed improved function compared to vehicle-treated animals in these tests. More strikingly, DHEA enhanced recovery of left-right coordination and fine motor control. In an attempt to correlate functional recovery with spinal cord neuropathology in the different experimental groups, we studied the area of spared white matter at the epicenter and reactive gliosis/scar formation 42 days post-injury (DPI). DHEA significantly increased the area of white matter spared at the epicenter and reduced the area of reactive gliosis surrounding the lesion. These data demonstrate the effectiveness of DHEA in promoting functional recovery in the adult murine injured spinal cord.

Original languageEnglish (US)
Pages (from-to)391-400
Number of pages10
JournalJournal of Neuroscience Research
Volume75
Issue number3
DOIs
StatePublished - Feb 1 2004
Externally publishedYes

Fingerprint

Dehydroepiandrosterone
Spinal Cord Injuries
Neurotransmitter Agents
Gliosis
Spinal Cord
Gait
Cicatrix
Foot
Embryonic Structures
Central Nervous System
Neurons
Wounds and Injuries
Brain
Growth

Keywords

  • Contusion
  • Dehydroepiandrosterone (DHEA)
  • Fine motor control
  • Functional recovery
  • Spinal cord injury (SCI)

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Treatment with the Neurosteroid Dehydroepiandrosterone Promotes Recovery of Motor Behavior after Moderate Contusive Spinal Cord Injury in the Mouse. / Fiore, Christelle; Inman, Denise M.; Hirose, Shinjiro; Noble, Linda J.; Igarashi, Takuji; Compagnone, Natalie A.

In: Journal of Neuroscience Research, Vol. 75, No. 3, 01.02.2004, p. 391-400.

Research output: Contribution to journalArticle

Fiore, Christelle ; Inman, Denise M. ; Hirose, Shinjiro ; Noble, Linda J. ; Igarashi, Takuji ; Compagnone, Natalie A. / Treatment with the Neurosteroid Dehydroepiandrosterone Promotes Recovery of Motor Behavior after Moderate Contusive Spinal Cord Injury in the Mouse. In: Journal of Neuroscience Research. 2004 ; Vol. 75, No. 3. pp. 391-400.
@article{4f147fdf92c041f9b15bc588408b4a1a,
title = "Treatment with the Neurosteroid Dehydroepiandrosterone Promotes Recovery of Motor Behavior after Moderate Contusive Spinal Cord Injury in the Mouse",
abstract = "The neurosteroid dehydroepiandrosterone (DHEA) has neuroprotective properties after ischemic and excitatory insults to the brain. In the developing embryo, it is produced in discrete regions of the central nervous system (CNS), where it specifically promotes axonal growth of differentiated neurons. To test if DHEA could be beneficial after spinal cord injury (SCI), we used a model of moderate contusive SCI developed and characterized in the mouse. Immediately after surgery, we applied treatment with DHEA or with vehicle only and compared treatment groups (n = 12 in each group) over a 42-day period. Locomotor recovery was assessed in an open field using a standardized 21-point scale, according to gait analysis on paw print recordings and using foot fault analyses on an inclined ladder beam. The DHEA-treated group showed improved function compared to vehicle-treated animals in these tests. More strikingly, DHEA enhanced recovery of left-right coordination and fine motor control. In an attempt to correlate functional recovery with spinal cord neuropathology in the different experimental groups, we studied the area of spared white matter at the epicenter and reactive gliosis/scar formation 42 days post-injury (DPI). DHEA significantly increased the area of white matter spared at the epicenter and reduced the area of reactive gliosis surrounding the lesion. These data demonstrate the effectiveness of DHEA in promoting functional recovery in the adult murine injured spinal cord.",
keywords = "Contusion, Dehydroepiandrosterone (DHEA), Fine motor control, Functional recovery, Spinal cord injury (SCI)",
author = "Christelle Fiore and Inman, {Denise M.} and Shinjiro Hirose and Noble, {Linda J.} and Takuji Igarashi and Compagnone, {Natalie A.}",
year = "2004",
month = "2",
day = "1",
doi = "10.1002/jnr.10821",
language = "English (US)",
volume = "75",
pages = "391--400",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Treatment with the Neurosteroid Dehydroepiandrosterone Promotes Recovery of Motor Behavior after Moderate Contusive Spinal Cord Injury in the Mouse

AU - Fiore, Christelle

AU - Inman, Denise M.

AU - Hirose, Shinjiro

AU - Noble, Linda J.

AU - Igarashi, Takuji

AU - Compagnone, Natalie A.

PY - 2004/2/1

Y1 - 2004/2/1

N2 - The neurosteroid dehydroepiandrosterone (DHEA) has neuroprotective properties after ischemic and excitatory insults to the brain. In the developing embryo, it is produced in discrete regions of the central nervous system (CNS), where it specifically promotes axonal growth of differentiated neurons. To test if DHEA could be beneficial after spinal cord injury (SCI), we used a model of moderate contusive SCI developed and characterized in the mouse. Immediately after surgery, we applied treatment with DHEA or with vehicle only and compared treatment groups (n = 12 in each group) over a 42-day period. Locomotor recovery was assessed in an open field using a standardized 21-point scale, according to gait analysis on paw print recordings and using foot fault analyses on an inclined ladder beam. The DHEA-treated group showed improved function compared to vehicle-treated animals in these tests. More strikingly, DHEA enhanced recovery of left-right coordination and fine motor control. In an attempt to correlate functional recovery with spinal cord neuropathology in the different experimental groups, we studied the area of spared white matter at the epicenter and reactive gliosis/scar formation 42 days post-injury (DPI). DHEA significantly increased the area of white matter spared at the epicenter and reduced the area of reactive gliosis surrounding the lesion. These data demonstrate the effectiveness of DHEA in promoting functional recovery in the adult murine injured spinal cord.

AB - The neurosteroid dehydroepiandrosterone (DHEA) has neuroprotective properties after ischemic and excitatory insults to the brain. In the developing embryo, it is produced in discrete regions of the central nervous system (CNS), where it specifically promotes axonal growth of differentiated neurons. To test if DHEA could be beneficial after spinal cord injury (SCI), we used a model of moderate contusive SCI developed and characterized in the mouse. Immediately after surgery, we applied treatment with DHEA or with vehicle only and compared treatment groups (n = 12 in each group) over a 42-day period. Locomotor recovery was assessed in an open field using a standardized 21-point scale, according to gait analysis on paw print recordings and using foot fault analyses on an inclined ladder beam. The DHEA-treated group showed improved function compared to vehicle-treated animals in these tests. More strikingly, DHEA enhanced recovery of left-right coordination and fine motor control. In an attempt to correlate functional recovery with spinal cord neuropathology in the different experimental groups, we studied the area of spared white matter at the epicenter and reactive gliosis/scar formation 42 days post-injury (DPI). DHEA significantly increased the area of white matter spared at the epicenter and reduced the area of reactive gliosis surrounding the lesion. These data demonstrate the effectiveness of DHEA in promoting functional recovery in the adult murine injured spinal cord.

KW - Contusion

KW - Dehydroepiandrosterone (DHEA)

KW - Fine motor control

KW - Functional recovery

KW - Spinal cord injury (SCI)

UR - http://www.scopus.com/inward/record.url?scp=0842312975&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0842312975&partnerID=8YFLogxK

U2 - 10.1002/jnr.10821

DO - 10.1002/jnr.10821

M3 - Article

VL - 75

SP - 391

EP - 400

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 3

ER -