Transposition without transposase

A spontaneous mutation in bacteria

Chad A. Rappleye, John R. Roth

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Transposition mutations are typically associated with the activities of transposable elements such as transposons and insertion sequences, whose mobility is dependent upon transposase enzymes that catalyze exchanges between element ends and target sites. We describe a single transposition event in which a block of donor sequence is inserted at a target site without the involvement of any known transposase or the ends of any known transposable element. We propose that this is a new type of spontaneous mutation which may be difficult to detect in standard mutant hunts but may be of evolutionary importance.

Original languageEnglish (US)
Pages (from-to)2047-2052
Number of pages6
JournalJournal of Bacteriology
Volume179
Issue number6
StatePublished - 1997
Externally publishedYes

Fingerprint

Transposases
DNA Transposable Elements
Bacteria
Mutation
Insertional Mutagenesis
Enzymes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Rappleye, C. A., & Roth, J. R. (1997). Transposition without transposase: A spontaneous mutation in bacteria. Journal of Bacteriology, 179(6), 2047-2052.

Transposition without transposase : A spontaneous mutation in bacteria. / Rappleye, Chad A.; Roth, John R.

In: Journal of Bacteriology, Vol. 179, No. 6, 1997, p. 2047-2052.

Research output: Contribution to journalArticle

Rappleye, CA & Roth, JR 1997, 'Transposition without transposase: A spontaneous mutation in bacteria', Journal of Bacteriology, vol. 179, no. 6, pp. 2047-2052.
Rappleye, Chad A. ; Roth, John R. / Transposition without transposase : A spontaneous mutation in bacteria. In: Journal of Bacteriology. 1997 ; Vol. 179, No. 6. pp. 2047-2052.
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