Transposition mutagenesis of bacteriophage lambda. A new gene affecting cell lysis

Ry Young, Jeffrey Way, Susan Way, Jerry Yin, Michael Syvanen

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Insertions of Tn903, a transposable kanamycin-resistance element, in bacteriophage lambda at 0.95 on the lambda physical map adversely affect growth of the phage. These insertion mutants are able to assemble particles, but are unable to lyse the infected cell properly. The mutants define a new genetic complementation group that we have designated as gene Rz. Cells infected with the λRz:: Tn903 isolates will, at the normal time of lysis, change their shape from a rod to a sphere. These spheres are stable in dilute buffers with Mg2+ but are lysed with EDTA. In addition, these results demonstrate the utility of transposition mutagenesis in refining the genetic map of even so intensely studied a genome as lambda.

Original languageEnglish (US)
Pages (from-to)307-322
Number of pages16
JournalJournal of Molecular Biology
Volume132
Issue number3
DOIs
StatePublished - Aug 15 1979
Externally publishedYes

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Bacteriophage lambda
Mutagenesis
Kanamycin Resistance
Edetic Acid
Bacteriophages
Genes
Buffers
Genome
Growth

ASJC Scopus subject areas

  • Virology

Cite this

Transposition mutagenesis of bacteriophage lambda. A new gene affecting cell lysis. / Young, Ry; Way, Jeffrey; Way, Susan; Yin, Jerry; Syvanen, Michael.

In: Journal of Molecular Biology, Vol. 132, No. 3, 15.08.1979, p. 307-322.

Research output: Contribution to journalArticle

Young, Ry ; Way, Jeffrey ; Way, Susan ; Yin, Jerry ; Syvanen, Michael. / Transposition mutagenesis of bacteriophage lambda. A new gene affecting cell lysis. In: Journal of Molecular Biology. 1979 ; Vol. 132, No. 3. pp. 307-322.
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