Abstract
Insertions of Tn903, a transposable kanamycin-resistance element, in bacteriophage lambda at 0.95 on the lambda physical map adversely affect growth of the phage. These insertion mutants are able to assemble particles, but are unable to lyse the infected cell properly. The mutants define a new genetic complementation group that we have designated as gene Rz. Cells infected with the λRz:: Tn903 isolates will, at the normal time of lysis, change their shape from a rod to a sphere. These spheres are stable in dilute buffers with Mg2+ but are lysed with EDTA. In addition, these results demonstrate the utility of transposition mutagenesis in refining the genetic map of even so intensely studied a genome as lambda.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 307-322 |
| Number of pages | 16 |
| Journal | Journal of Molecular Biology |
| Volume | 132 |
| Issue number | 3 |
| DOIs | |
| State | Published - Aug 15 1979 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Virology
Cite this
Transposition mutagenesis of bacteriophage lambda. A new gene affecting cell lysis. / Young, Ry; Way, Jeffrey; Way, Susan; Yin, Jerry; Syvanen, Michael.
In: Journal of Molecular Biology, Vol. 132, No. 3, 15.08.1979, p. 307-322.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transposition mutagenesis of bacteriophage lambda. A new gene affecting cell lysis
AU - Young, Ry
AU - Way, Jeffrey
AU - Way, Susan
AU - Yin, Jerry
AU - Syvanen, Michael
PY - 1979/8/15
Y1 - 1979/8/15
N2 - Insertions of Tn903, a transposable kanamycin-resistance element, in bacteriophage lambda at 0.95 on the lambda physical map adversely affect growth of the phage. These insertion mutants are able to assemble particles, but are unable to lyse the infected cell properly. The mutants define a new genetic complementation group that we have designated as gene Rz. Cells infected with the λRz:: Tn903 isolates will, at the normal time of lysis, change their shape from a rod to a sphere. These spheres are stable in dilute buffers with Mg2+ but are lysed with EDTA. In addition, these results demonstrate the utility of transposition mutagenesis in refining the genetic map of even so intensely studied a genome as lambda.
AB - Insertions of Tn903, a transposable kanamycin-resistance element, in bacteriophage lambda at 0.95 on the lambda physical map adversely affect growth of the phage. These insertion mutants are able to assemble particles, but are unable to lyse the infected cell properly. The mutants define a new genetic complementation group that we have designated as gene Rz. Cells infected with the λRz:: Tn903 isolates will, at the normal time of lysis, change their shape from a rod to a sphere. These spheres are stable in dilute buffers with Mg2+ but are lysed with EDTA. In addition, these results demonstrate the utility of transposition mutagenesis in refining the genetic map of even so intensely studied a genome as lambda.
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UR - http://www.scopus.com/inward/citedby.url?scp=0018791980&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(79)90262-6
DO - 10.1016/0022-2836(79)90262-6
M3 - Article
VL - 132
SP - 307
EP - 322
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 3
ER -