Abstract
In this experiment we evaluated the transmission characteristics of a chicken-adapted low-pathogenicity avian influenza virus (LPAIV) of subtype H6N2, from infected chickens to Japanese quail and Pekin ducks, which are commonly sold in live bird markets located in Southern California. The layout of the cages and bird-handling practices were similar to those found in Southern California live bird markets. Five out of 20 chickens were inoculated with LPAIV H6N2, and placed in direct contact with five chickens and in indirect contact with 10 chickens, 10 Japanese quail and 10 Pekin ducks. Transmission of LPAIV was followed in each bird daily for 15 days post inoculation by real-time reverse transcriptase-polymerase chain reaction testing of oropharyngeal and cloacal swab samples. This strain of H6N2 LPAIV, isolated from commercial poultry in California, was transmitted to chickens, quail, and ducks from chickens. An antibody response was detected in ducks by haemagglutination inhibition tests, but avian influenza virus was only detected by reverse transcriptase-polymerase chain reaction in one duck. Avian influenza virus was detected in quail (5 and 7 days post inoculation) before chickens (8 and 9 days post inoculation), all of which were in indirect contact with infected chickens; however, this difference was not statistically significant.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 59-64 |
| Number of pages | 6 |
| Journal | Avian Pathology |
| Volume | 38 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1 2009 |
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ASJC Scopus subject areas
- Food Animals
- Animal Science and Zoology
- Immunology and Microbiology(all)
Cite this
Transmission of low-pathogenicity avian influenza virus of subtype H6N2 from chickens to Pekin ducks and Japanese quail (Coturnix coturnix japonica). / Yee, Karen S.; Cardona, Carol J.; Carpenter, Tim.
In: Avian Pathology, Vol. 38, No. 1, 01.02.2009, p. 59-64.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transmission of low-pathogenicity avian influenza virus of subtype H6N2 from chickens to Pekin ducks and Japanese quail (Coturnix coturnix japonica)
AU - Yee, Karen S.
AU - Cardona, Carol J.
AU - Carpenter, Tim
PY - 2009/2/1
Y1 - 2009/2/1
N2 - In this experiment we evaluated the transmission characteristics of a chicken-adapted low-pathogenicity avian influenza virus (LPAIV) of subtype H6N2, from infected chickens to Japanese quail and Pekin ducks, which are commonly sold in live bird markets located in Southern California. The layout of the cages and bird-handling practices were similar to those found in Southern California live bird markets. Five out of 20 chickens were inoculated with LPAIV H6N2, and placed in direct contact with five chickens and in indirect contact with 10 chickens, 10 Japanese quail and 10 Pekin ducks. Transmission of LPAIV was followed in each bird daily for 15 days post inoculation by real-time reverse transcriptase-polymerase chain reaction testing of oropharyngeal and cloacal swab samples. This strain of H6N2 LPAIV, isolated from commercial poultry in California, was transmitted to chickens, quail, and ducks from chickens. An antibody response was detected in ducks by haemagglutination inhibition tests, but avian influenza virus was only detected by reverse transcriptase-polymerase chain reaction in one duck. Avian influenza virus was detected in quail (5 and 7 days post inoculation) before chickens (8 and 9 days post inoculation), all of which were in indirect contact with infected chickens; however, this difference was not statistically significant.
AB - In this experiment we evaluated the transmission characteristics of a chicken-adapted low-pathogenicity avian influenza virus (LPAIV) of subtype H6N2, from infected chickens to Japanese quail and Pekin ducks, which are commonly sold in live bird markets located in Southern California. The layout of the cages and bird-handling practices were similar to those found in Southern California live bird markets. Five out of 20 chickens were inoculated with LPAIV H6N2, and placed in direct contact with five chickens and in indirect contact with 10 chickens, 10 Japanese quail and 10 Pekin ducks. Transmission of LPAIV was followed in each bird daily for 15 days post inoculation by real-time reverse transcriptase-polymerase chain reaction testing of oropharyngeal and cloacal swab samples. This strain of H6N2 LPAIV, isolated from commercial poultry in California, was transmitted to chickens, quail, and ducks from chickens. An antibody response was detected in ducks by haemagglutination inhibition tests, but avian influenza virus was only detected by reverse transcriptase-polymerase chain reaction in one duck. Avian influenza virus was detected in quail (5 and 7 days post inoculation) before chickens (8 and 9 days post inoculation), all of which were in indirect contact with infected chickens; however, this difference was not statistically significant.
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U2 - 10.1080/03079450802632023
DO - 10.1080/03079450802632023
M3 - Article
VL - 38
SP - 59
EP - 64
JO - Avian Pathology
JF - Avian Pathology
SN - 0307-9457
IS - 1
ER -