Abstract
In late 2014, Zika virus (ZIKV; Flaviviridae, Flavivirus) emerged as a significant arboviral disease threat in the Western hemisphere. Aedes aegypti and Aedes albopictus have been considered the principal vectors of ZIKV in the New World due to viral isolation frequency and vector competence assessments. Limited reports of Culex transmission potential have highlighted the need for additional vector competence assessments of North American Culex species. Accordingly, North American Culex pipiens and Culex quinquefasciatus were orally exposed and intrathoracically inoculated with the African prototype ZIKV strain and currently circulating Asian lineage ZIKV strains to assess infection, dissemination, and transmission potential. Results indicated that these two North American Culex mosquito species were highly refractory to oral infection with no dissemination or transmission observed with any ZIKV strains assessed. Furthermore, both Culex mosquito species intrathoracically inoculated with either Asian or African lineage ZIKVs failed to expectorate virus in saliva. These in vivo results were further supported by the observation that multiple mosquito cell lines of Culex species origin demonstrated significant growth restriction of ZIKV strains compared with Aedes-derived cell lines. In summation, no evidence for the potential of Cx. pipiens or Cx. quinquefasciatus to serve as a competent vector for ZIKV transmission in North America was observed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1235-1240 |
| Number of pages | 6 |
| Journal | American Journal of Tropical Medicine and Hygiene |
| Volume | 96 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jan 1 2017 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Parasitology
- Infectious Diseases
- Virology
Cite this
Transmission incompetence of culex quinquefasciatus and culex pipiens pipiens from North America for zika virus. / Kenney, Joan L.; Romo, Hannah; Duggal, Nisha K.; Tzeng, Wen Pin; Burkhalter, Kristen L.; Brault, Aaron; Savage, Harry M.
In: American Journal of Tropical Medicine and Hygiene, Vol. 96, No. 5, 01.01.2017, p. 1235-1240.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transmission incompetence of culex quinquefasciatus and culex pipiens pipiens from North America for zika virus
AU - Kenney, Joan L.
AU - Romo, Hannah
AU - Duggal, Nisha K.
AU - Tzeng, Wen Pin
AU - Burkhalter, Kristen L.
AU - Brault, Aaron
AU - Savage, Harry M.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - In late 2014, Zika virus (ZIKV; Flaviviridae, Flavivirus) emerged as a significant arboviral disease threat in the Western hemisphere. Aedes aegypti and Aedes albopictus have been considered the principal vectors of ZIKV in the New World due to viral isolation frequency and vector competence assessments. Limited reports of Culex transmission potential have highlighted the need for additional vector competence assessments of North American Culex species. Accordingly, North American Culex pipiens and Culex quinquefasciatus were orally exposed and intrathoracically inoculated with the African prototype ZIKV strain and currently circulating Asian lineage ZIKV strains to assess infection, dissemination, and transmission potential. Results indicated that these two North American Culex mosquito species were highly refractory to oral infection with no dissemination or transmission observed with any ZIKV strains assessed. Furthermore, both Culex mosquito species intrathoracically inoculated with either Asian or African lineage ZIKVs failed to expectorate virus in saliva. These in vivo results were further supported by the observation that multiple mosquito cell lines of Culex species origin demonstrated significant growth restriction of ZIKV strains compared with Aedes-derived cell lines. In summation, no evidence for the potential of Cx. pipiens or Cx. quinquefasciatus to serve as a competent vector for ZIKV transmission in North America was observed.
AB - In late 2014, Zika virus (ZIKV; Flaviviridae, Flavivirus) emerged as a significant arboviral disease threat in the Western hemisphere. Aedes aegypti and Aedes albopictus have been considered the principal vectors of ZIKV in the New World due to viral isolation frequency and vector competence assessments. Limited reports of Culex transmission potential have highlighted the need for additional vector competence assessments of North American Culex species. Accordingly, North American Culex pipiens and Culex quinquefasciatus were orally exposed and intrathoracically inoculated with the African prototype ZIKV strain and currently circulating Asian lineage ZIKV strains to assess infection, dissemination, and transmission potential. Results indicated that these two North American Culex mosquito species were highly refractory to oral infection with no dissemination or transmission observed with any ZIKV strains assessed. Furthermore, both Culex mosquito species intrathoracically inoculated with either Asian or African lineage ZIKVs failed to expectorate virus in saliva. These in vivo results were further supported by the observation that multiple mosquito cell lines of Culex species origin demonstrated significant growth restriction of ZIKV strains compared with Aedes-derived cell lines. In summation, no evidence for the potential of Cx. pipiens or Cx. quinquefasciatus to serve as a competent vector for ZIKV transmission in North America was observed.
UR - http://www.scopus.com/inward/record.url?scp=85019133844&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85019133844&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.16-0865
DO - 10.4269/ajtmh.16-0865
M3 - Article
C2 - 28500817
AN - SCOPUS:85019133844
VL - 96
SP - 1235
EP - 1240
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
SN - 0002-9637
IS - 5
ER -