Translational profiling of retinal ganglion cell optic nerve regeneration in Xenopus laevis

G. B. Whitworth, B. C. Misaghi, D. M. Rosenthal, E. A. Mills, D. J. Heinen, A. H. Watson, C. W. Ives, S. H. Ali, K. Bezold, Nicholas Marsh-Armstrong, F. L. Watson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Unlike adult mammals, adult frogs regrow their optic nerve following a crush injury, making Xenopus laevis a compelling model for studying the molecular mechanisms that underlie neuronal regeneration. Using Translational Ribosome Affinity Purification (TRAP), a method to isolate ribosome-associated mRNAs from a target cell population, we have generated a transcriptional profile by RNA-Seq for retinal ganglion cells (RGC) during the period of recovery following an optic nerve injury. Based on bioinformatic analysis using the Xenopus laevis 9.1 genome assembly, our results reveal a profound shift in the composition of ribosome-associated mRNAs during the early stages of RGC regeneration. As factors involved in cell signaling are rapidly down-regulated, those involved in protein biosynthesis are up-regulated alongside key initiators of axon development. Using the new genome assembly, we were also able to analyze gene expression profiles of homeologous gene pairs arising from a whole-genome duplication in the Xenopus lineage. Here we see evidence of divergence in regulatory control among a significant proportion of pairs. Our data should provide a valuable resource for identifying genes involved in the regeneration process to target for future functional studies, in both naturally regenerative and non-regenerative vertebrates.

Original languageEnglish (US)
Pages (from-to)360-373
Number of pages14
JournalDevelopmental Biology
Volume426
Issue number2
DOIs
StatePublished - Jun 15 2017
Externally publishedYes

Fingerprint

Nerve Regeneration
Retinal Ganglion Cells
Xenopus laevis
Optic Nerve
Ribosomes
Regeneration
Genome
Optic Nerve Injuries
Messenger RNA
Molecular Models
Health Services Needs and Demand
Protein Biosynthesis
Xenopus
Computational Biology
Transcriptome
Anura
Genes
Axons
Vertebrates
Mammals

Keywords

  • Expression profile
  • Optic nerve crush injury
  • TRAP

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Whitworth, G. B., Misaghi, B. C., Rosenthal, D. M., Mills, E. A., Heinen, D. J., Watson, A. H., ... Watson, F. L. (2017). Translational profiling of retinal ganglion cell optic nerve regeneration in Xenopus laevis. Developmental Biology, 426(2), 360-373. https://doi.org/10.1016/j.ydbio.2016.06.003

Translational profiling of retinal ganglion cell optic nerve regeneration in Xenopus laevis. / Whitworth, G. B.; Misaghi, B. C.; Rosenthal, D. M.; Mills, E. A.; Heinen, D. J.; Watson, A. H.; Ives, C. W.; Ali, S. H.; Bezold, K.; Marsh-Armstrong, Nicholas; Watson, F. L.

In: Developmental Biology, Vol. 426, No. 2, 15.06.2017, p. 360-373.

Research output: Contribution to journalArticle

Whitworth, GB, Misaghi, BC, Rosenthal, DM, Mills, EA, Heinen, DJ, Watson, AH, Ives, CW, Ali, SH, Bezold, K, Marsh-Armstrong, N & Watson, FL 2017, 'Translational profiling of retinal ganglion cell optic nerve regeneration in Xenopus laevis', Developmental Biology, vol. 426, no. 2, pp. 360-373. https://doi.org/10.1016/j.ydbio.2016.06.003
Whitworth GB, Misaghi BC, Rosenthal DM, Mills EA, Heinen DJ, Watson AH et al. Translational profiling of retinal ganglion cell optic nerve regeneration in Xenopus laevis. Developmental Biology. 2017 Jun 15;426(2):360-373. https://doi.org/10.1016/j.ydbio.2016.06.003
Whitworth, G. B. ; Misaghi, B. C. ; Rosenthal, D. M. ; Mills, E. A. ; Heinen, D. J. ; Watson, A. H. ; Ives, C. W. ; Ali, S. H. ; Bezold, K. ; Marsh-Armstrong, Nicholas ; Watson, F. L. / Translational profiling of retinal ganglion cell optic nerve regeneration in Xenopus laevis. In: Developmental Biology. 2017 ; Vol. 426, No. 2. pp. 360-373.
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