Translational effects of peptide antagonists of Tat protein of human immunodeficiency virus type 1

Indrani Choudhury, Jihong Wang, Stanley Stein, Arnold Rabson, Michael J Leibowitz

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The Tat (trans-activator of transcription) regulatory protein of human immunodeficiency virus (HIV-1) acts by interacting with the TAR RNA domain of nascent viral transcripts and with cellular proteins to increase viral transcription. In Jurkat-derived HCLE-D36 cells, which are stably transfected with the chloramphenicol acetyltransferase (CAT) reporter gene expressed from the TAR-encoding long terminal repeat (LTR) of HIV-1, CAT protein expression is dependent on Tat. The Tat9-K-biotin peptide antagonist of Tat binds specifically to TAR RNA and competes with Tat for binding. In the cellular expression system, Tat9-K-biotin reduces Tat-dependent CAT expression. However, while the Tat antagonist greatly reduces CAT protein production and polysome association of CAT mRNA, it has little effect on CAT mRNA levels, suggesting that the antagonist works at the post-transcriptional level.

Original languageEnglish (US)
Pages (from-to)777-782
Number of pages6
JournalJournal of General Virology
Volume80
Issue number3
StatePublished - 1999
Externally publishedYes

ASJC Scopus subject areas

  • Virology
  • Immunology

Fingerprint Dive into the research topics of 'Translational effects of peptide antagonists of Tat protein of human immunodeficiency virus type 1'. Together they form a unique fingerprint.

  • Cite this