Abstract
A stable mRNA-dependent cell-free translation system from Saccharomyces cerevisiae, prepared by a modification of the method of Hofbauer et al. [Eur. J. Biochem. 122 (1982) 199-203] was active in translation of exogenous homologous and heterologous mRNAs. Optimal translational activity required the addition of polyamines and yeast tRNA. The m transcript of the M segment of double-stranded RNA, synthesized in vitro using the killer virus-associated RNA polymerase, directed the synthesis of preprotoxin polypeptide (M-p32), which was immunologically identified using antitoxin antibody. Sindbis virus capsid protein and rabbit globin were also translated from their mRNAs. Translation was inhibited by puromycin, sparsomycin and anisomycin. Analogues of the 5'-terminal caps present on most eukaryotic mRNA molecules inhibited translation of added mRNAs, including capped mRNAs and the uncapped killer virus mRNA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 13-23 |
| Number of pages | 11 |
| Journal | Gene |
| Volume | 46 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1986 |
| Externally published | Yes |
Fingerprint
Keywords
- cap analogues
- double-stranded RNA
- killer virus
- preprotoxin
- Protein synthesis
- recombinant DNA
- RNA polymerase
- Saccharomyces cerevisiae
ASJC Scopus subject areas
- Genetics
Cite this
Translation of homologous and heterologous messenger RNAs in a yeast cell-free system. / Hussain, Iffat; Leibowitz, Michael J.
In: Gene, Vol. 46, No. 1, 1986, p. 13-23.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Translation of homologous and heterologous messenger RNAs in a yeast cell-free system
AU - Hussain, Iffat
AU - Leibowitz, Michael J
PY - 1986
Y1 - 1986
N2 - A stable mRNA-dependent cell-free translation system from Saccharomyces cerevisiae, prepared by a modification of the method of Hofbauer et al. [Eur. J. Biochem. 122 (1982) 199-203] was active in translation of exogenous homologous and heterologous mRNAs. Optimal translational activity required the addition of polyamines and yeast tRNA. The m transcript of the M segment of double-stranded RNA, synthesized in vitro using the killer virus-associated RNA polymerase, directed the synthesis of preprotoxin polypeptide (M-p32), which was immunologically identified using antitoxin antibody. Sindbis virus capsid protein and rabbit globin were also translated from their mRNAs. Translation was inhibited by puromycin, sparsomycin and anisomycin. Analogues of the 5'-terminal caps present on most eukaryotic mRNA molecules inhibited translation of added mRNAs, including capped mRNAs and the uncapped killer virus mRNA.
AB - A stable mRNA-dependent cell-free translation system from Saccharomyces cerevisiae, prepared by a modification of the method of Hofbauer et al. [Eur. J. Biochem. 122 (1982) 199-203] was active in translation of exogenous homologous and heterologous mRNAs. Optimal translational activity required the addition of polyamines and yeast tRNA. The m transcript of the M segment of double-stranded RNA, synthesized in vitro using the killer virus-associated RNA polymerase, directed the synthesis of preprotoxin polypeptide (M-p32), which was immunologically identified using antitoxin antibody. Sindbis virus capsid protein and rabbit globin were also translated from their mRNAs. Translation was inhibited by puromycin, sparsomycin and anisomycin. Analogues of the 5'-terminal caps present on most eukaryotic mRNA molecules inhibited translation of added mRNAs, including capped mRNAs and the uncapped killer virus mRNA.
KW - cap analogues
KW - double-stranded RNA
KW - killer virus
KW - preprotoxin
KW - Protein synthesis
KW - recombinant DNA
KW - RNA polymerase
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=0022873934&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022873934&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(86)90162-9
DO - 10.1016/0378-1119(86)90162-9
M3 - Article
VL - 46
SP - 13
EP - 23
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1
ER -