Translation of homologous and heterologous messenger RNAs in a yeast cell-free system

Iffat Hussain; Michael J Leibowitz

doi:10.1016/0378-1119(86)90162-9

Translation of homologous and heterologous messenger RNAs in a yeast cell-free system

Iffat Hussain, Michael J Leibowitz

Research output: Contribution to journal › Article

24 Scopus citations

Abstract

A stable mRNA-dependent cell-free translation system from Saccharomyces cerevisiae, prepared by a modification of the method of Hofbauer et al. [Eur. J. Biochem. 122 (1982) 199-203] was active in translation of exogenous homologous and heterologous mRNAs. Optimal translational activity required the addition of polyamines and yeast tRNA. The m transcript of the M segment of double-stranded RNA, synthesized in vitro using the killer virus-associated RNA polymerase, directed the synthesis of preprotoxin polypeptide (M-p32), which was immunologically identified using antitoxin antibody. Sindbis virus capsid protein and rabbit globin were also translated from their mRNAs. Translation was inhibited by puromycin, sparsomycin and anisomycin. Analogues of the 5'-terminal caps present on most eukaryotic mRNA molecules inhibited translation of added mRNAs, including capped mRNAs and the uncapped killer virus mRNA.

Original language	English (US)
Pages (from-to)	13-23
Number of pages	11
Journal	Gene
Volume	46
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(86)90162-9
State	Published - 1986
Externally published	Yes

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Keywords

cap analogues
double-stranded RNA
killer virus
preprotoxin
Protein synthesis
recombinant DNA
RNA polymerase
Saccharomyces cerevisiae

ASJC Scopus subject areas

Genetics

Cite this

Hussain, I., & Leibowitz, M. J. (1986). Translation of homologous and heterologous messenger RNAs in a yeast cell-free system. Gene, 46(1), 13-23. https://doi.org/10.1016/0378-1119(86)90162-9

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Access to Document

10.1016/0378-1119(86)90162-9