Transitional changes in arachidonic acid metabolism by bovine embryos at different developmental stages

D. H. Hwang, S. H. Pool, R. W. Rorie, M. Boudreau, R. A. Godke

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In order to determine the profile of arachidonic acid (AA) metabolites synthesized by bovine embryos during early developmental stages, embryos collected from superovulated beef cattle (days 6 through 17) were incubated with AA and its metabolites were analyzed by high performance liquid chromatography and radioimmunoassay (RIA). Embryos harvested and cultured before day 12 of the estrous cycle metabolized AA primarily to prostaglandin E2 (PGE2), whereas, those harvested on day 13 of the cycle metabolized AA to both PGE2 and PGF2α. Furthermore, embryos collected after day 15 of the cycle metabolized AA to PGI2 in addition to PGE2 and PGF2α. In view of the luteotropic properties that have been attributed to PGE2 and the vasodilatory effect of PGI2, this transitional change in prostaglandin synthesis during early stages of embryonic development may be a part of the mechanism by which the embryo exerts a luteotropic effect leading to maternal recognition of pregnancy and by which the conceptus begins preparing for subsequent implantation.

Original languageEnglish (US)
Pages (from-to)387-402
Number of pages16
JournalProstaglandins
Volume35
Issue number3
DOIs
StatePublished - 1988
Externally publishedYes

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Arachidonic Acid
Metabolism
Dinoprostone
Embryonic Structures
Dinoprost
Epoprostenol
Metabolites
Beef
Estrous Cycle
High performance liquid chromatography
Prostaglandins
Embryonic Development
Radioimmunoassay
High Pressure Liquid Chromatography
Mothers
Pregnancy

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Transitional changes in arachidonic acid metabolism by bovine embryos at different developmental stages. / Hwang, D. H.; Pool, S. H.; Rorie, R. W.; Boudreau, M.; Godke, R. A.

In: Prostaglandins, Vol. 35, No. 3, 1988, p. 387-402.

Research output: Contribution to journalArticle

Hwang, D. H. ; Pool, S. H. ; Rorie, R. W. ; Boudreau, M. ; Godke, R. A. / Transitional changes in arachidonic acid metabolism by bovine embryos at different developmental stages. In: Prostaglandins. 1988 ; Vol. 35, No. 3. pp. 387-402.
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