Transitional cell cytokeratins as a second parameter in flow cytometry of bladder cancer

B. F. Edwards, P. V. Rossitto, W. C. Baker, Robert Cardiff, M. Strand, A. D. Deitch, Ralph W deVere White

Research output: Contribution to journalArticle

Abstract

Since the detection of abnormal DNA content in the cells of transitional cell carcinoma can be obscured by the presence in the population of diploid stromal and inflammatory cells, it would be desirable to have a second flow cytometric parameter to complement DNA analysis. The specific identification of carcinoma cells using monoclonal antibodies directed against epithelial intermediate filament proteins (cytokeratins) would provide a method with which to identify the transitional cells in solid tumors, voided urines or bladder washings. As a preliminary study we have tested cell-free urine samples using a double antibody radiometric assay (IRMAK) for cytokeratins released from transitional cells. The additional information from cytokeratin analyses increased the specificity of disease detection.

Original languageEnglish (US)
Pages (from-to)123-126
Number of pages4
JournalWorld Journal of Urology
Volume5
Issue number2
DOIs
StatePublished - May 1987

ASJC Scopus subject areas

  • Urology

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