Transition state analogs as ligands for affinity purification of juvenile hormone esterase

Yehia A I Abdel-Aal, Bruce D. Hammock

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Insect juvenile hormones are metabolized in numerous species of caterpillars by low abundance, highly specific esterases. Because of their role in regulating and possibly disrupting juvenile hormone titer and thus insect metamorphosis, they are of interest to developmental biologists as well as scientists interested in selective insect control. However, the enzymes have defied attempts to purify and characterize them. Juvenile hormone esterase activity can be inhibited by a variety of 3-substituted 1,1,1-trifluoropropanone sulfides. These apparent transition state analogs were used as ligands and eluting agents to purify juvenile hormone esterase from four insect species from 500-fold to over 1000-fold in high yield. After elution from the affinity column, the enzymes were radiolabeled with paraoxon and analyzed by electrophoresis, and the results demonstrate a high degree ofpurity. Transition state analogs may be useful for the affinity purification of other enzymes.

Original languageEnglish (US)
Pages (from-to)1073-1076
Number of pages4
JournalScience
Volume233
Issue number4768
StatePublished - 1986

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Juvenile Hormones
Ligands
Insects
Enzymes
Insect Hormones
Insect Control
Paraoxon
Sulfides
Esterases
Electrophoresis
juvenile hormone esterase

ASJC Scopus subject areas

  • General

Cite this

Abdel-Aal, Y. A. I., & Hammock, B. D. (1986). Transition state analogs as ligands for affinity purification of juvenile hormone esterase. Science, 233(4768), 1073-1076.

Transition state analogs as ligands for affinity purification of juvenile hormone esterase. / Abdel-Aal, Yehia A I; Hammock, Bruce D.

In: Science, Vol. 233, No. 4768, 1986, p. 1073-1076.

Research output: Contribution to journalArticle

Abdel-Aal, YAI & Hammock, BD 1986, 'Transition state analogs as ligands for affinity purification of juvenile hormone esterase', Science, vol. 233, no. 4768, pp. 1073-1076.
Abdel-Aal, Yehia A I ; Hammock, Bruce D. / Transition state analogs as ligands for affinity purification of juvenile hormone esterase. In: Science. 1986 ; Vol. 233, No. 4768. pp. 1073-1076.
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