Transient permeabilization of airway epithelium by mucosal water

Jonathan Widdicombe, F. Azizi, T. Kang, J. F. Pittet

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We describe a simple hyposmotic shock procedure whereby the apical membrane of airway epithelium can be made transiently leaky to proteins and other macromolecules. Bovine or human tracheal epithelial cells were grown as confluent polarized cell sheets on porous inserts. While physiological saline was maintained on the basolateral surface, the mucosal surface was exposed to water. This led to marked increases in the uptake of [14C]mannitol across both apical and basolateral membranes. On restoring saline to the mucosal surface, the [14C]mannitol permeability returned to preexposure levels with a half-life of ~5 min. Mucosal water also increased efflux of lactate dehydrogenase and the uptakes of fluorescent albumin and dextran (2,000 kDa). Water-induced increases in mannitol permeability were similar at 4 and 37°C, suggesting that pinocytosis was not the mechanism. Detailed time courses of the uptake of dextran and the loss of lactate dehydrogenase and 36Cl showed that the bulk of the permeability increase occurred during the first 2- to 4- min exposure to water. Transepithelial resistance was reversibly decreased by exposure to water, but short-circuit current responses to transport blockers and secretagogues remained qualitatively normal. The hyposmotic shock procedure also successfully permeabilized apical membranes of primary cultures of nasal epithelial cells from a patient with cystic fibrosis (CF) and of JME/CF 15 cells, a cell line derived from CF bronchial epithelium. This simple and efficient procedure may prove useful in studies on the cell and molecular biology of airway and other epithelia.

Original languageEnglish (US)
Pages (from-to)491-499
Number of pages9
JournalJournal of Applied Physiology
Volume81
Issue number1
StatePublished - Jul 1996

Fingerprint

Epithelium
Mannitol
Cystic Fibrosis
Water
Permeability
Dextrans
L-Lactate Dehydrogenase
Membranes
Shock
Epithelial Cells
Pinocytosis
Nose
Half-Life
Cell Biology
Molecular Biology
Albumins
Cell Line
Proteins

Keywords

  • cystic fibrosis
  • hyposmotic shock
  • lactate dehydrogenase
  • membrane permeability

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Transient permeabilization of airway epithelium by mucosal water. / Widdicombe, Jonathan; Azizi, F.; Kang, T.; Pittet, J. F.

In: Journal of Applied Physiology, Vol. 81, No. 1, 07.1996, p. 491-499.

Research output: Contribution to journalArticle

Widdicombe, J, Azizi, F, Kang, T & Pittet, JF 1996, 'Transient permeabilization of airway epithelium by mucosal water', Journal of Applied Physiology, vol. 81, no. 1, pp. 491-499.
Widdicombe, Jonathan ; Azizi, F. ; Kang, T. ; Pittet, J. F. / Transient permeabilization of airway epithelium by mucosal water. In: Journal of Applied Physiology. 1996 ; Vol. 81, No. 1. pp. 491-499.
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