Transient pearling and vesiculation of membrane tubes under osmotic gradients

Jeremy Sanborn, Kamila Oglȩcka, Rachel S. Kraut, Atul N. Parikh

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We report the experimental observation of osmotically induced transient pearling instabilities in vesicular membranes. Giant phospholipid vesicles subjected to negative osmotic gradient, which drives the influx of water in to the vesicular interior, produces transient cylindrical protrusions. These protrusions exhibit a remarkable pearling intermediate, which facilitates their subsequent retraction. The pearling front propagates from the distal free end of the protrusion toward the vesicular source and accompanies gradual shortening of the protrusion via pearl-pearl coalescence. Real-time introduction of a positive osmotic gradient, on the other hand, drives vigorous shape fluctuations, which in turn produce cylindrical, prolate- and pear-shaped intermediates presumably due to an increased vesicular area relative to the encapsulated volume. These intermediates transiently produce a pearled state prior to their fission. In both cases, the transient pearling state gives rise to an array of stable spherical daughter vesicles, which may be connected to one another by fine tethers not resolved in our experiments. These results may have implications for self-reproduction in primitive, protein-free, cells. This journal is

Original languageEnglish (US)
Pages (from-to)167-176
Number of pages10
JournalFaraday Discussions
Volume161
DOIs
StatePublished - Dec 10 2012

Fingerprint

tubes
membranes
Membranes
gradients
Phosmet
coalescing
fission
proteins
Coalescence
Phospholipids
cells
water
Water
Proteins
Experiments

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Transient pearling and vesiculation of membrane tubes under osmotic gradients. / Sanborn, Jeremy; Oglȩcka, Kamila; Kraut, Rachel S.; Parikh, Atul N.

In: Faraday Discussions, Vol. 161, 10.12.2012, p. 167-176.

Research output: Contribution to journalArticle

Sanborn, Jeremy ; Oglȩcka, Kamila ; Kraut, Rachel S. ; Parikh, Atul N. / Transient pearling and vesiculation of membrane tubes under osmotic gradients. In: Faraday Discussions. 2012 ; Vol. 161. pp. 167-176.
@article{0475cc1a3f47437ab011285fd7f5a3fa,
title = "Transient pearling and vesiculation of membrane tubes under osmotic gradients",
abstract = "We report the experimental observation of osmotically induced transient pearling instabilities in vesicular membranes. Giant phospholipid vesicles subjected to negative osmotic gradient, which drives the influx of water in to the vesicular interior, produces transient cylindrical protrusions. These protrusions exhibit a remarkable pearling intermediate, which facilitates their subsequent retraction. The pearling front propagates from the distal free end of the protrusion toward the vesicular source and accompanies gradual shortening of the protrusion via pearl-pearl coalescence. Real-time introduction of a positive osmotic gradient, on the other hand, drives vigorous shape fluctuations, which in turn produce cylindrical, prolate- and pear-shaped intermediates presumably due to an increased vesicular area relative to the encapsulated volume. These intermediates transiently produce a pearled state prior to their fission. In both cases, the transient pearling state gives rise to an array of stable spherical daughter vesicles, which may be connected to one another by fine tethers not resolved in our experiments. These results may have implications for self-reproduction in primitive, protein-free, cells. This journal is",
author = "Jeremy Sanborn and Kamila Oglȩcka and Kraut, {Rachel S.} and Parikh, {Atul N.}",
year = "2012",
month = "12",
day = "10",
doi = "10.1039/c2fd20116j",
language = "English (US)",
volume = "161",
pages = "167--176",
journal = "Faraday Discussions of the Chemical Society",
issn = "1364-5498",
publisher = "Royal Society of Chemistry",

}

TY - JOUR

T1 - Transient pearling and vesiculation of membrane tubes under osmotic gradients

AU - Sanborn, Jeremy

AU - Oglȩcka, Kamila

AU - Kraut, Rachel S.

AU - Parikh, Atul N.

PY - 2012/12/10

Y1 - 2012/12/10

N2 - We report the experimental observation of osmotically induced transient pearling instabilities in vesicular membranes. Giant phospholipid vesicles subjected to negative osmotic gradient, which drives the influx of water in to the vesicular interior, produces transient cylindrical protrusions. These protrusions exhibit a remarkable pearling intermediate, which facilitates their subsequent retraction. The pearling front propagates from the distal free end of the protrusion toward the vesicular source and accompanies gradual shortening of the protrusion via pearl-pearl coalescence. Real-time introduction of a positive osmotic gradient, on the other hand, drives vigorous shape fluctuations, which in turn produce cylindrical, prolate- and pear-shaped intermediates presumably due to an increased vesicular area relative to the encapsulated volume. These intermediates transiently produce a pearled state prior to their fission. In both cases, the transient pearling state gives rise to an array of stable spherical daughter vesicles, which may be connected to one another by fine tethers not resolved in our experiments. These results may have implications for self-reproduction in primitive, protein-free, cells. This journal is

AB - We report the experimental observation of osmotically induced transient pearling instabilities in vesicular membranes. Giant phospholipid vesicles subjected to negative osmotic gradient, which drives the influx of water in to the vesicular interior, produces transient cylindrical protrusions. These protrusions exhibit a remarkable pearling intermediate, which facilitates their subsequent retraction. The pearling front propagates from the distal free end of the protrusion toward the vesicular source and accompanies gradual shortening of the protrusion via pearl-pearl coalescence. Real-time introduction of a positive osmotic gradient, on the other hand, drives vigorous shape fluctuations, which in turn produce cylindrical, prolate- and pear-shaped intermediates presumably due to an increased vesicular area relative to the encapsulated volume. These intermediates transiently produce a pearled state prior to their fission. In both cases, the transient pearling state gives rise to an array of stable spherical daughter vesicles, which may be connected to one another by fine tethers not resolved in our experiments. These results may have implications for self-reproduction in primitive, protein-free, cells. This journal is

UR - http://www.scopus.com/inward/record.url?scp=84875709109&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875709109&partnerID=8YFLogxK

U2 - 10.1039/c2fd20116j

DO - 10.1039/c2fd20116j

M3 - Article

C2 - 23805742

AN - SCOPUS:84875709109

VL - 161

SP - 167

EP - 176

JO - Faraday Discussions of the Chemical Society

JF - Faraday Discussions of the Chemical Society

SN - 1364-5498

ER -