Abstract
We report the experimental observation of osmotically induced transient pearling instabilities in vesicular membranes. Giant phospholipid vesicles subjected to negative osmotic gradient, which drives the influx of water in to the vesicular interior, produces transient cylindrical protrusions. These protrusions exhibit a remarkable pearling intermediate, which facilitates their subsequent retraction. The pearling front propagates from the distal free end of the protrusion toward the vesicular source and accompanies gradual shortening of the protrusion via pearl-pearl coalescence. Real-time introduction of a positive osmotic gradient, on the other hand, drives vigorous shape fluctuations, which in turn produce cylindrical, prolate- and pear-shaped intermediates presumably due to an increased vesicular area relative to the encapsulated volume. These intermediates transiently produce a pearled state prior to their fission. In both cases, the transient pearling state gives rise to an array of stable spherical daughter vesicles, which may be connected to one another by fine tethers not resolved in our experiments. These results may have implications for self-reproduction in primitive, protein-free, cells. This journal is
| Original language | English (US) |
|---|---|
| Pages (from-to) | 167-176 |
| Number of pages | 10 |
| Journal | Faraday Discussions |
| Volume | 161 |
| DOIs | |
| State | Published - Dec 10 2012 |
Fingerprint
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
Cite this
Transient pearling and vesiculation of membrane tubes under osmotic gradients. / Sanborn, Jeremy; Oglȩcka, Kamila; Kraut, Rachel S.; Parikh, Atul N.
In: Faraday Discussions, Vol. 161, 10.12.2012, p. 167-176.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transient pearling and vesiculation of membrane tubes under osmotic gradients
AU - Sanborn, Jeremy
AU - Oglȩcka, Kamila
AU - Kraut, Rachel S.
AU - Parikh, Atul N.
PY - 2012/12/10
Y1 - 2012/12/10
N2 - We report the experimental observation of osmotically induced transient pearling instabilities in vesicular membranes. Giant phospholipid vesicles subjected to negative osmotic gradient, which drives the influx of water in to the vesicular interior, produces transient cylindrical protrusions. These protrusions exhibit a remarkable pearling intermediate, which facilitates their subsequent retraction. The pearling front propagates from the distal free end of the protrusion toward the vesicular source and accompanies gradual shortening of the protrusion via pearl-pearl coalescence. Real-time introduction of a positive osmotic gradient, on the other hand, drives vigorous shape fluctuations, which in turn produce cylindrical, prolate- and pear-shaped intermediates presumably due to an increased vesicular area relative to the encapsulated volume. These intermediates transiently produce a pearled state prior to their fission. In both cases, the transient pearling state gives rise to an array of stable spherical daughter vesicles, which may be connected to one another by fine tethers not resolved in our experiments. These results may have implications for self-reproduction in primitive, protein-free, cells. This journal is
AB - We report the experimental observation of osmotically induced transient pearling instabilities in vesicular membranes. Giant phospholipid vesicles subjected to negative osmotic gradient, which drives the influx of water in to the vesicular interior, produces transient cylindrical protrusions. These protrusions exhibit a remarkable pearling intermediate, which facilitates their subsequent retraction. The pearling front propagates from the distal free end of the protrusion toward the vesicular source and accompanies gradual shortening of the protrusion via pearl-pearl coalescence. Real-time introduction of a positive osmotic gradient, on the other hand, drives vigorous shape fluctuations, which in turn produce cylindrical, prolate- and pear-shaped intermediates presumably due to an increased vesicular area relative to the encapsulated volume. These intermediates transiently produce a pearled state prior to their fission. In both cases, the transient pearling state gives rise to an array of stable spherical daughter vesicles, which may be connected to one another by fine tethers not resolved in our experiments. These results may have implications for self-reproduction in primitive, protein-free, cells. This journal is
UR - http://www.scopus.com/inward/record.url?scp=84875709109&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875709109&partnerID=8YFLogxK
U2 - 10.1039/c2fd20116j
DO - 10.1039/c2fd20116j
M3 - Article
C2 - 23805742
AN - SCOPUS:84875709109
VL - 161
SP - 167
EP - 176
JO - Faraday Discussions of the Chemical Society
JF - Faraday Discussions of the Chemical Society
SN - 1364-5498
ER -