Transient overexpression of γ interferon promotes Aspergillus clearance in invasive pulmonary aspergillosis

C. Shao, Jieming Qu, L. He, Y. Zhang, J. Wang, Y. Wang, H. Zhou, X. Liu

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Cytokines are critical molecules necessary for normal lung pathogen host defences. Gamma interferon (IFN-γ) and T1-phenotype immune responses are important components of host defence against Aspergillus. Therefore, we hypothesized that transient overexpression of IFN-γ within the lung could augment host immunity against Aspergillus. Here it was showed that intranasal administration of 5 × 107 colony-forming units (CFU) of Aspergillus fumigatus (Af) induced the expression of IFN-γ. Mice were intranasally (i.n) administrated with 5 × 108 PFU of a recombinant adenovirus vector containing the murine IFN-γ cDNA (AdmIFN-γ), and challenged 24 h later with Af. We observed that i.n. administration of AdmIFN-γ resulted in about a fourfold increase in levels of IFN-γ and IL-12 within the lung, about a 75% reduction in lung fungal contents at day 2 and a more than threefold higher survival rate in the AdmIFN-γ-treated group compared to the controls (P < 001). This protection effect was not found when AdmIFN-γ was i.p. administrated. Alveolar macrophages and lung leucocytes isolated from i.n. AdmIFN-γ- treated animals displayed enhanced killing of intracellular Aspergillus organisms ex vivo. These results demonstrate that transient overexpression of IFN-γ could augment host defence against Aspergillus.

Original languageEnglish (US)
Pages (from-to)233-241
Number of pages9
JournalClinical and Experimental Immunology
Volume142
Issue number2
DOIs
StatePublished - Nov 2005
Externally publishedYes

Keywords

  • Adenovirus
  • Fungi
  • Gene therapy
  • Interferon γ
  • Invasive pulmonary aspergillosis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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