Transient MRI-detected water apparent diffusion coefficient reduction correlates with c-fos mRNA but not hsp70 mRNA induction during focal cerebral ischemia in rats

Anthony Mancuso, Nikita Derugin, Yasuki Ono, Kazushi Hara, Frank R Sharp, Philip R. Weinstein

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Cerebral ischemia induces immediate early genes such as c-fos and stress genes such as hsp70. In this study, the spatial relationships between c-fos and hsp70 mRNA expression and changes detectable with diffusion and perfusion magnetic resonance (MR) imaging were examined. The middle cerebral artery (MCA) of young adult rats was occluded for 30 or 60 min. Diffusion MR (D-MR) images were acquired continuously during the ischemic period and dysprosium- contrast perfusion (P-MR) images were acquired at the end of the ischemic period. C-fos and hsp70 mRNA expression were examined with in situ hybridization. The most significant finding of this work was that for both durations of ischemia, c-fos induction was observed in cortical and sub- cortical regions exhibiting a transient reduction in the apparent diffusion coefficient of water (ADC). Transients which occurred on a time scale of 3 min may have been caused by spreading depression. Those occurring on a 10-min time scale may have been caused by an initial reduction in blood flow with occlusion that was followed by an ischemia-induced increase in collateral blood flow. P-MR imaging showed that perfusion in c-fos positive regions was higher than in regions with persistently reduced ADC. Hsp70 induction did not correlate with transient ADC reduction. It was induced in the MCA territory in regions showing persistent ADC changes, with induction being greatest at the periphery of these regions. It was also induced in regions that exhibited both spontaneous reversal of the diffusion changes and decreased perfusion.

Original languageEnglish (US)
Pages (from-to)7-22
Number of pages16
JournalBrain Research
Volume839
Issue number1
DOIs
StatePublished - Aug 21 1999
Externally publishedYes

Fingerprint

Brain Ischemia
Messenger RNA
Water
Magnetic Resonance Angiography
Middle Cerebral Artery
Dysprosium
Magnetic Resonance Spectroscopy
Ischemia
Perfusion
fos Genes
Diffusion Magnetic Resonance Imaging
Immediate-Early Genes
In Situ Hybridization
Young Adult

Keywords

  • C-fos
  • Diffusion MRI
  • Focal cerebral ischemia
  • Hsp70
  • Immediate early gene
  • Stress gene

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Transient MRI-detected water apparent diffusion coefficient reduction correlates with c-fos mRNA but not hsp70 mRNA induction during focal cerebral ischemia in rats. / Mancuso, Anthony; Derugin, Nikita; Ono, Yasuki; Hara, Kazushi; Sharp, Frank R; Weinstein, Philip R.

In: Brain Research, Vol. 839, No. 1, 21.08.1999, p. 7-22.

Research output: Contribution to journalArticle

Mancuso, Anthony ; Derugin, Nikita ; Ono, Yasuki ; Hara, Kazushi ; Sharp, Frank R ; Weinstein, Philip R. / Transient MRI-detected water apparent diffusion coefficient reduction correlates with c-fos mRNA but not hsp70 mRNA induction during focal cerebral ischemia in rats. In: Brain Research. 1999 ; Vol. 839, No. 1. pp. 7-22.
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