Transient Genome-Wide Transcriptional Response to Low-Dose Ionizing Radiation In Vivo in Humans

Susanne R. Berglund, David M Rocke, Jian Dai, Chad W. Schwietert, Alison Santana, Robin L Stern, Joerg Lehmann, Christine L. Hartmann Siantar, Zelanna Goldberg

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


Purpose: The in vivo effects of low-dose low linear energy transfer ionizing radiation on healthy human skin are largely unknown. Using a patient-based tissue acquisition protocol, we have performed a series of genomic analyses on the temporal dynamics over a 24-hour period to determine the radiation response after a single exposure of 10 cGy. Methods and Materials: RNA from each patient tissue sample was hybridized to an Affymetrix Human Genome U133 Plus 2.0 array. Data analysis was performed on selected gene groups and pathways. Results: Nineteen gene groups and seven gene pathways that had been shown to be radiation responsive were analyzed. Of these, nine gene groups showed significant transient transcriptional changes in the human tissue samples, which returned to baseline by 24 hours postexposure. Conclusions: Low doses of ionizing radiation on full-thickness human skin produce a definable temporal response out to 24 hours postexposure. Genes involved in DNA and tissue remodeling, cell cycle transition, and inflammation show statistically significant changes in expression, despite variability between patients. These data serve as a reference for the temporal dynamics of ionizing radiation response following low-dose exposure in healthy full-thickness human skin.

Original languageEnglish (US)
Pages (from-to)229-234
Number of pages6
JournalInternational Journal of Radiation Oncology Biology Physics
Issue number1
StatePublished - Jan 1 2008


  • Biosignature
  • Genomic expression patterns
  • Human
  • In vivo dosimetry
  • Low-dose ionizing radiation

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Radiation


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