Transient expression analysis of the reticuloendotheliosis virus long terminal repeat element

Anthony A G Ridgway, Hsing-Jien Kung, Donald J. Fujita

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A region of the Reticuloendotheliosis virus (REV) long terminal repeat (LTR) harbouring single or duplicated copies of 46-bp and 26-bp sequence elements is implicated in enhancer activity. Sequences residing upstream from the proviral 3′ LTR did not contribute to activity of the intact LTR. Gene expression regulated by a combination of REV enhancer and SV40 early region promoter was 50-fold less than from the analogous construct containing the chicken syncytial virus promoter. Deletion of LTR sequences immediately downstream of the CAP site, which include a region capable of forming a stable hairpin in the mRNA, decreased expression by 70%. Expression assays and S1 nuclease mapping showed that a second transcriptional start site, identified by transcription in vitro using HeLa cell lysates and purified DNA templates, was not used in vivo in the cell lines examined.

Original languageEnglish (US)
Pages (from-to)3199-3215
Number of pages17
JournalNucleic Acids Research
Volume17
Issue number8
DOIs
StatePublished - Apr 25 1989
Externally publishedYes

Fingerprint

Reticuloendotheliosis virus
Terminal Repeat Sequences
Viruses
Virus
Promoter
Cell
Transcription
Gene expression
Messenger RNA
Deletion
Gene Expression
Immediately
Template
Assays
Fold
DNA
Cells
Transcription Initiation Site
HeLa Cells
Genetic Promoter Regions

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Transient expression analysis of the reticuloendotheliosis virus long terminal repeat element. / Ridgway, Anthony A G; Kung, Hsing-Jien; Fujita, Donald J.

In: Nucleic Acids Research, Vol. 17, No. 8, 25.04.1989, p. 3199-3215.

Research output: Contribution to journalArticle

@article{64da89093a4848fd942f70c011e906de,
title = "Transient expression analysis of the reticuloendotheliosis virus long terminal repeat element",
abstract = "A region of the Reticuloendotheliosis virus (REV) long terminal repeat (LTR) harbouring single or duplicated copies of 46-bp and 26-bp sequence elements is implicated in enhancer activity. Sequences residing upstream from the proviral 3′ LTR did not contribute to activity of the intact LTR. Gene expression regulated by a combination of REV enhancer and SV40 early region promoter was 50-fold less than from the analogous construct containing the chicken syncytial virus promoter. Deletion of LTR sequences immediately downstream of the CAP site, which include a region capable of forming a stable hairpin in the mRNA, decreased expression by 70{\%}. Expression assays and S1 nuclease mapping showed that a second transcriptional start site, identified by transcription in vitro using HeLa cell lysates and purified DNA templates, was not used in vivo in the cell lines examined.",
author = "Ridgway, {Anthony A G} and Hsing-Jien Kung and Fujita, {Donald J.}",
year = "1989",
month = "4",
day = "25",
doi = "10.1093/nar/17.8.3199",
language = "English (US)",
volume = "17",
pages = "3199--3215",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "8",

}

TY - JOUR

T1 - Transient expression analysis of the reticuloendotheliosis virus long terminal repeat element

AU - Ridgway, Anthony A G

AU - Kung, Hsing-Jien

AU - Fujita, Donald J.

PY - 1989/4/25

Y1 - 1989/4/25

N2 - A region of the Reticuloendotheliosis virus (REV) long terminal repeat (LTR) harbouring single or duplicated copies of 46-bp and 26-bp sequence elements is implicated in enhancer activity. Sequences residing upstream from the proviral 3′ LTR did not contribute to activity of the intact LTR. Gene expression regulated by a combination of REV enhancer and SV40 early region promoter was 50-fold less than from the analogous construct containing the chicken syncytial virus promoter. Deletion of LTR sequences immediately downstream of the CAP site, which include a region capable of forming a stable hairpin in the mRNA, decreased expression by 70%. Expression assays and S1 nuclease mapping showed that a second transcriptional start site, identified by transcription in vitro using HeLa cell lysates and purified DNA templates, was not used in vivo in the cell lines examined.

AB - A region of the Reticuloendotheliosis virus (REV) long terminal repeat (LTR) harbouring single or duplicated copies of 46-bp and 26-bp sequence elements is implicated in enhancer activity. Sequences residing upstream from the proviral 3′ LTR did not contribute to activity of the intact LTR. Gene expression regulated by a combination of REV enhancer and SV40 early region promoter was 50-fold less than from the analogous construct containing the chicken syncytial virus promoter. Deletion of LTR sequences immediately downstream of the CAP site, which include a region capable of forming a stable hairpin in the mRNA, decreased expression by 70%. Expression assays and S1 nuclease mapping showed that a second transcriptional start site, identified by transcription in vitro using HeLa cell lysates and purified DNA templates, was not used in vivo in the cell lines examined.

UR - http://www.scopus.com/inward/record.url?scp=0024520012&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024520012&partnerID=8YFLogxK

U2 - 10.1093/nar/17.8.3199

DO - 10.1093/nar/17.8.3199

M3 - Article

C2 - 2542893

AN - SCOPUS:0024520012

VL - 17

SP - 3199

EP - 3215

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 8

ER -