Abstract
Objective To determine if binge ethanol consumption before ovulation affects oocyte quality, gene expression, and subsequent embryo development. Design Binge levels of ethanol were given twice weekly for 6 months, followed by a standard in vitro fertilization cycle and subsequent natural mating. Setting National primate research center. Animal(s) Adult female rhesus monkeys. Intervention(s) Binge levels of ethanol, given twice weekly for 6 months before a standard in vitro fertilization cycle with or without embryo culture. With in vivo development, ethanol treatment continued until pregnancy was identified. Main Outcome Measure(s) Oocyte and cumulus/granulosa cell gene expression, embryo development to blastocyst, and pregnancy rate. Result(s) Embryo development in vitro was reduced; changes were found in oocyte and cumulus cell gene expression; and spontaneous abortion during very early gestation increased. Conclusion(s) This study provides evidence that binge drinking can affect the developmental potential of oocytes even after alcohol consumption has ceased.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 560-569 |
| Number of pages | 10 |
| Journal | Fertility and Sterility |
| Volume | 103 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2015 |
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Keywords
- cDNA array
- Cumulus cells
- fetal alcohol syndrome
- granulosa cells
- reproduction
- transcriptome
ASJC Scopus subject areas
- Obstetrics and Gynecology
- Reproductive Medicine
Cite this
Transgenerational effects of binge drinking in a primate model : Implications for human health. / Vandevoort, Catherine A.; Grimsrud, Kristin N; Midic, Uros; Mtango, Namdori; Latham, Keith E.
In: Fertility and Sterility, Vol. 103, No. 2, 01.02.2015, p. 560-569.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transgenerational effects of binge drinking in a primate model
T2 - Implications for human health
AU - Vandevoort, Catherine A.
AU - Grimsrud, Kristin N
AU - Midic, Uros
AU - Mtango, Namdori
AU - Latham, Keith E.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Objective To determine if binge ethanol consumption before ovulation affects oocyte quality, gene expression, and subsequent embryo development. Design Binge levels of ethanol were given twice weekly for 6 months, followed by a standard in vitro fertilization cycle and subsequent natural mating. Setting National primate research center. Animal(s) Adult female rhesus monkeys. Intervention(s) Binge levels of ethanol, given twice weekly for 6 months before a standard in vitro fertilization cycle with or without embryo culture. With in vivo development, ethanol treatment continued until pregnancy was identified. Main Outcome Measure(s) Oocyte and cumulus/granulosa cell gene expression, embryo development to blastocyst, and pregnancy rate. Result(s) Embryo development in vitro was reduced; changes were found in oocyte and cumulus cell gene expression; and spontaneous abortion during very early gestation increased. Conclusion(s) This study provides evidence that binge drinking can affect the developmental potential of oocytes even after alcohol consumption has ceased.
AB - Objective To determine if binge ethanol consumption before ovulation affects oocyte quality, gene expression, and subsequent embryo development. Design Binge levels of ethanol were given twice weekly for 6 months, followed by a standard in vitro fertilization cycle and subsequent natural mating. Setting National primate research center. Animal(s) Adult female rhesus monkeys. Intervention(s) Binge levels of ethanol, given twice weekly for 6 months before a standard in vitro fertilization cycle with or without embryo culture. With in vivo development, ethanol treatment continued until pregnancy was identified. Main Outcome Measure(s) Oocyte and cumulus/granulosa cell gene expression, embryo development to blastocyst, and pregnancy rate. Result(s) Embryo development in vitro was reduced; changes were found in oocyte and cumulus cell gene expression; and spontaneous abortion during very early gestation increased. Conclusion(s) This study provides evidence that binge drinking can affect the developmental potential of oocytes even after alcohol consumption has ceased.
KW - cDNA array
KW - Cumulus cells
KW - fetal alcohol syndrome
KW - granulosa cells
KW - reproduction
KW - transcriptome
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UR - http://www.scopus.com/inward/citedby.url?scp=84923226220&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2014.10.051
DO - 10.1016/j.fertnstert.2014.10.051
M3 - Article
C2 - 25492684
AN - SCOPUS:84923226220
VL - 103
SP - 560
EP - 569
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 2
ER -