Abstract
Transforming growth factor-alpha (TGF-α) is a biologically potent polypeptide detected in the gastrointestinal tract in suckling rats. The major goal of the present study was to test the hypothesis that the administration of TGF-α affects gastric emptying and small intestinal transit in suckling rats. Suckling (12-day-old) rats fasted 16 h received rat TGF-α subcutaneously (s.c.) or orogastrically in varying doses (0, 0.5, 1.0 μg/rat in 0.1% BSA). Control animals received 0.1% BSA only. Poly R-478 dye was used as a motility marker. Rats were decapitated 45 min after marker administration and the amount of dye in the stomach and the small intestine was measured by spectrophotometry. Subcutaneous administration of TGF-α significantly delayed stomach evacuation. In controls, the stomach contained 21.4 ± 1.4% (mean ± sx) of the Poly R-478 marker, whereas in TGF-α treated rats the stomach contained 37.2 ± 2.8% of the total Poly R-478 dye given to animals. The delaying effect of TGF-α was time and dose-dependent. Small intestinal transit was also significantly delayed. The proximal jejunum of TGF-α treated rats contained a 1.4-fold higher amount of marker in comparison with control rats. Orogastrically administered rTGF-α did not affect gastric emptying or intestinal transit. In conclusion, s.c. administration of rat TGF-α significantly delayed the gastrointestinal motility in vivo in suckling rats.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 356-360 |
| Number of pages | 5 |
| Journal | Scandinavian Journal of Gastroenterology |
| Volume | 36 |
| Issue number | 4 |
| State | Published - 2001 |
| Externally published | Yes |
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Keywords
- Gastrointestinal motility
- Intestinal transit
- Suckling rats
ASJC Scopus subject areas
- Gastroenterology
Cite this
Transforming growth factor-alpha delays gastric emptying and small intestinal transit in suckling rats. / Shinohara, H.; Williams, C. S.; McWilliam, D. L.; Koldovskýy, O.; Philipps, Anthony F; Dvořák, B.
In: Scandinavian Journal of Gastroenterology, Vol. 36, No. 4, 2001, p. 356-360.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transforming growth factor-alpha delays gastric emptying and small intestinal transit in suckling rats
AU - Shinohara, H.
AU - Williams, C. S.
AU - McWilliam, D. L.
AU - Koldovskýy, O.
AU - Philipps, Anthony F
AU - Dvořák, B.
PY - 2001
Y1 - 2001
N2 - Transforming growth factor-alpha (TGF-α) is a biologically potent polypeptide detected in the gastrointestinal tract in suckling rats. The major goal of the present study was to test the hypothesis that the administration of TGF-α affects gastric emptying and small intestinal transit in suckling rats. Suckling (12-day-old) rats fasted 16 h received rat TGF-α subcutaneously (s.c.) or orogastrically in varying doses (0, 0.5, 1.0 μg/rat in 0.1% BSA). Control animals received 0.1% BSA only. Poly R-478 dye was used as a motility marker. Rats were decapitated 45 min after marker administration and the amount of dye in the stomach and the small intestine was measured by spectrophotometry. Subcutaneous administration of TGF-α significantly delayed stomach evacuation. In controls, the stomach contained 21.4 ± 1.4% (mean ± sx) of the Poly R-478 marker, whereas in TGF-α treated rats the stomach contained 37.2 ± 2.8% of the total Poly R-478 dye given to animals. The delaying effect of TGF-α was time and dose-dependent. Small intestinal transit was also significantly delayed. The proximal jejunum of TGF-α treated rats contained a 1.4-fold higher amount of marker in comparison with control rats. Orogastrically administered rTGF-α did not affect gastric emptying or intestinal transit. In conclusion, s.c. administration of rat TGF-α significantly delayed the gastrointestinal motility in vivo in suckling rats.
AB - Transforming growth factor-alpha (TGF-α) is a biologically potent polypeptide detected in the gastrointestinal tract in suckling rats. The major goal of the present study was to test the hypothesis that the administration of TGF-α affects gastric emptying and small intestinal transit in suckling rats. Suckling (12-day-old) rats fasted 16 h received rat TGF-α subcutaneously (s.c.) or orogastrically in varying doses (0, 0.5, 1.0 μg/rat in 0.1% BSA). Control animals received 0.1% BSA only. Poly R-478 dye was used as a motility marker. Rats were decapitated 45 min after marker administration and the amount of dye in the stomach and the small intestine was measured by spectrophotometry. Subcutaneous administration of TGF-α significantly delayed stomach evacuation. In controls, the stomach contained 21.4 ± 1.4% (mean ± sx) of the Poly R-478 marker, whereas in TGF-α treated rats the stomach contained 37.2 ± 2.8% of the total Poly R-478 dye given to animals. The delaying effect of TGF-α was time and dose-dependent. Small intestinal transit was also significantly delayed. The proximal jejunum of TGF-α treated rats contained a 1.4-fold higher amount of marker in comparison with control rats. Orogastrically administered rTGF-α did not affect gastric emptying or intestinal transit. In conclusion, s.c. administration of rat TGF-α significantly delayed the gastrointestinal motility in vivo in suckling rats.
KW - Gastrointestinal motility
KW - Intestinal transit
KW - Suckling rats
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UR - http://www.scopus.com/inward/citedby.url?scp=0035091704&partnerID=8YFLogxK
M3 - Article
VL - 36
SP - 356
EP - 360
JO - Scandinavian Journal of Gastroenterology
JF - Scandinavian Journal of Gastroenterology
SN - 0036-5521
IS - 4
ER -