Transforming growth factor-alpha delays gastric emptying and small intestinal transit in suckling rats

H. Shinohara, C. S. Williams, D. L. McWilliam, O. Koldovskýy, Anthony F Philipps, B. Dvořák

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Transforming growth factor-alpha (TGF-α) is a biologically potent polypeptide detected in the gastrointestinal tract in suckling rats. The major goal of the present study was to test the hypothesis that the administration of TGF-α affects gastric emptying and small intestinal transit in suckling rats. Suckling (12-day-old) rats fasted 16 h received rat TGF-α subcutaneously (s.c.) or orogastrically in varying doses (0, 0.5, 1.0 μg/rat in 0.1% BSA). Control animals received 0.1% BSA only. Poly R-478 dye was used as a motility marker. Rats were decapitated 45 min after marker administration and the amount of dye in the stomach and the small intestine was measured by spectrophotometry. Subcutaneous administration of TGF-α significantly delayed stomach evacuation. In controls, the stomach contained 21.4 ± 1.4% (mean ± sx) of the Poly R-478 marker, whereas in TGF-α treated rats the stomach contained 37.2 ± 2.8% of the total Poly R-478 dye given to animals. The delaying effect of TGF-α was time and dose-dependent. Small intestinal transit was also significantly delayed. The proximal jejunum of TGF-α treated rats contained a 1.4-fold higher amount of marker in comparison with control rats. Orogastrically administered rTGF-α did not affect gastric emptying or intestinal transit. In conclusion, s.c. administration of rat TGF-α significantly delayed the gastrointestinal motility in vivo in suckling rats.

Original languageEnglish (US)
Pages (from-to)356-360
Number of pages5
JournalScandinavian Journal of Gastroenterology
Volume36
Issue number4
StatePublished - 2001
Externally publishedYes

Keywords

  • Gastrointestinal motility
  • Intestinal transit
  • Suckling rats

ASJC Scopus subject areas

  • Gastroenterology

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    Shinohara, H., Williams, C. S., McWilliam, D. L., Koldovskýy, O., Philipps, A. F., & Dvořák, B. (2001). Transforming growth factor-alpha delays gastric emptying and small intestinal transit in suckling rats. Scandinavian Journal of Gastroenterology, 36(4), 356-360.