Transforming growth factor β in bovine placentas

Linda Munson, Alice Wilhite, Valerie F. Boltz, J. Erby Wilkinson

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Transforming growth factor βs (TGFβ) are a family of multifunctional growth factors that are important embryonic morphogens. Because TGFβs may regulate the development of epitheliochorial placentas, we investigated the location, expression, secretion, and effects of TGFβs in bovine placentomes and cell cultures derived from chorionic and endometrial epithelia. Placentomes from early second-trimester pregnancies were examined by immunohistochemistry for TGFβ1, TGFβ2, and TGFβ3, and for TGFβ expression in Northern slot-blots. Effects of TGFβs were assessed in trophoblastic and endometrial epithelial cell lines by DNA synthesis assays. Secretion of TGFβs by trophoblastic and endometrial epithelial cells was determined using bioassays. All forms of TGFβ were immunolocalized in bovine placentomes. TGFβ mRNA was expressed in chorioallantois, caruncles, and in cultured trophoblastic and endometrial epithelial cells. Endometrial and trophoblastic cells secreted active and latent TGFβs, and these cells had a transient proliferative response to all forms of TGFβ. These results indicate that TGFβs are present at the fetal-maternal interface of the bovine placentome and may promote endometrial and chorionic growth.

Original languageEnglish (US)
Pages (from-to)748-755
Number of pages8
JournalBiology of Reproduction
Volume55
Issue number4
StatePublished - Oct 1996
Externally publishedYes

Fingerprint

Transforming Growth Factors
Placenta
Epithelial Cells
Second Pregnancy Trimester
Biological Assay
Northern Blotting
Intercellular Signaling Peptides and Proteins
Epithelium
Cell Culture Techniques
Immunohistochemistry
Mothers
Cell Line
Messenger RNA
DNA
Growth

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Munson, L., Wilhite, A., Boltz, V. F., & Wilkinson, J. E. (1996). Transforming growth factor β in bovine placentas. Biology of Reproduction, 55(4), 748-755.

Transforming growth factor β in bovine placentas. / Munson, Linda; Wilhite, Alice; Boltz, Valerie F.; Wilkinson, J. Erby.

In: Biology of Reproduction, Vol. 55, No. 4, 10.1996, p. 748-755.

Research output: Contribution to journalArticle

Munson, L, Wilhite, A, Boltz, VF & Wilkinson, JE 1996, 'Transforming growth factor β in bovine placentas', Biology of Reproduction, vol. 55, no. 4, pp. 748-755.
Munson L, Wilhite A, Boltz VF, Wilkinson JE. Transforming growth factor β in bovine placentas. Biology of Reproduction. 1996 Oct;55(4):748-755.
Munson, Linda ; Wilhite, Alice ; Boltz, Valerie F. ; Wilkinson, J. Erby. / Transforming growth factor β in bovine placentas. In: Biology of Reproduction. 1996 ; Vol. 55, No. 4. pp. 748-755.
@article{2c636a61bc434785a49b11ff70e52964,
title = "Transforming growth factor β in bovine placentas",
abstract = "Transforming growth factor βs (TGFβ) are a family of multifunctional growth factors that are important embryonic morphogens. Because TGFβs may regulate the development of epitheliochorial placentas, we investigated the location, expression, secretion, and effects of TGFβs in bovine placentomes and cell cultures derived from chorionic and endometrial epithelia. Placentomes from early second-trimester pregnancies were examined by immunohistochemistry for TGFβ1, TGFβ2, and TGFβ3, and for TGFβ expression in Northern slot-blots. Effects of TGFβs were assessed in trophoblastic and endometrial epithelial cell lines by DNA synthesis assays. Secretion of TGFβs by trophoblastic and endometrial epithelial cells was determined using bioassays. All forms of TGFβ were immunolocalized in bovine placentomes. TGFβ mRNA was expressed in chorioallantois, caruncles, and in cultured trophoblastic and endometrial epithelial cells. Endometrial and trophoblastic cells secreted active and latent TGFβs, and these cells had a transient proliferative response to all forms of TGFβ. These results indicate that TGFβs are present at the fetal-maternal interface of the bovine placentome and may promote endometrial and chorionic growth.",
author = "Linda Munson and Alice Wilhite and Boltz, {Valerie F.} and Wilkinson, {J. Erby}",
year = "1996",
month = "10",
language = "English (US)",
volume = "55",
pages = "748--755",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "4",

}

TY - JOUR

T1 - Transforming growth factor β in bovine placentas

AU - Munson, Linda

AU - Wilhite, Alice

AU - Boltz, Valerie F.

AU - Wilkinson, J. Erby

PY - 1996/10

Y1 - 1996/10

N2 - Transforming growth factor βs (TGFβ) are a family of multifunctional growth factors that are important embryonic morphogens. Because TGFβs may regulate the development of epitheliochorial placentas, we investigated the location, expression, secretion, and effects of TGFβs in bovine placentomes and cell cultures derived from chorionic and endometrial epithelia. Placentomes from early second-trimester pregnancies were examined by immunohistochemistry for TGFβ1, TGFβ2, and TGFβ3, and for TGFβ expression in Northern slot-blots. Effects of TGFβs were assessed in trophoblastic and endometrial epithelial cell lines by DNA synthesis assays. Secretion of TGFβs by trophoblastic and endometrial epithelial cells was determined using bioassays. All forms of TGFβ were immunolocalized in bovine placentomes. TGFβ mRNA was expressed in chorioallantois, caruncles, and in cultured trophoblastic and endometrial epithelial cells. Endometrial and trophoblastic cells secreted active and latent TGFβs, and these cells had a transient proliferative response to all forms of TGFβ. These results indicate that TGFβs are present at the fetal-maternal interface of the bovine placentome and may promote endometrial and chorionic growth.

AB - Transforming growth factor βs (TGFβ) are a family of multifunctional growth factors that are important embryonic morphogens. Because TGFβs may regulate the development of epitheliochorial placentas, we investigated the location, expression, secretion, and effects of TGFβs in bovine placentomes and cell cultures derived from chorionic and endometrial epithelia. Placentomes from early second-trimester pregnancies were examined by immunohistochemistry for TGFβ1, TGFβ2, and TGFβ3, and for TGFβ expression in Northern slot-blots. Effects of TGFβs were assessed in trophoblastic and endometrial epithelial cell lines by DNA synthesis assays. Secretion of TGFβs by trophoblastic and endometrial epithelial cells was determined using bioassays. All forms of TGFβ were immunolocalized in bovine placentomes. TGFβ mRNA was expressed in chorioallantois, caruncles, and in cultured trophoblastic and endometrial epithelial cells. Endometrial and trophoblastic cells secreted active and latent TGFβs, and these cells had a transient proliferative response to all forms of TGFβ. These results indicate that TGFβs are present at the fetal-maternal interface of the bovine placentome and may promote endometrial and chorionic growth.

UR - http://www.scopus.com/inward/record.url?scp=0029787507&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029787507&partnerID=8YFLogxK

M3 - Article

C2 - 8879485

AN - SCOPUS:0029787507

VL - 55

SP - 748

EP - 755

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 4

ER -