Abstract
Transforming growth factor βs (TGFβ) are a family of multifunctional growth factors that are important embryonic morphogens. Because TGFβs may regulate the development of epitheliochorial placentas, we investigated the location, expression, secretion, and effects of TGFβs in bovine placentomes and cell cultures derived from chorionic and endometrial epithelia. Placentomes from early second-trimester pregnancies were examined by immunohistochemistry for TGFβ1, TGFβ2, and TGFβ3, and for TGFβ expression in Northern slot-blots. Effects of TGFβs were assessed in trophoblastic and endometrial epithelial cell lines by DNA synthesis assays. Secretion of TGFβs by trophoblastic and endometrial epithelial cells was determined using bioassays. All forms of TGFβ were immunolocalized in bovine placentomes. TGFβ mRNA was expressed in chorioallantois, caruncles, and in cultured trophoblastic and endometrial epithelial cells. Endometrial and trophoblastic cells secreted active and latent TGFβs, and these cells had a transient proliferative response to all forms of TGFβ. These results indicate that TGFβs are present at the fetal-maternal interface of the bovine placentome and may promote endometrial and chorionic growth.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 748-755 |
| Number of pages | 8 |
| Journal | Biology of Reproduction |
| Volume | 55 |
| Issue number | 4 |
| State | Published - Oct 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
- Developmental Biology
- Embryology
Cite this
Transforming growth factor β in bovine placentas. / Munson, Linda; Wilhite, Alice; Boltz, Valerie F.; Wilkinson, J. Erby.
In: Biology of Reproduction, Vol. 55, No. 4, 10.1996, p. 748-755.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transforming growth factor β in bovine placentas
AU - Munson, Linda
AU - Wilhite, Alice
AU - Boltz, Valerie F.
AU - Wilkinson, J. Erby
PY - 1996/10
Y1 - 1996/10
N2 - Transforming growth factor βs (TGFβ) are a family of multifunctional growth factors that are important embryonic morphogens. Because TGFβs may regulate the development of epitheliochorial placentas, we investigated the location, expression, secretion, and effects of TGFβs in bovine placentomes and cell cultures derived from chorionic and endometrial epithelia. Placentomes from early second-trimester pregnancies were examined by immunohistochemistry for TGFβ1, TGFβ2, and TGFβ3, and for TGFβ expression in Northern slot-blots. Effects of TGFβs were assessed in trophoblastic and endometrial epithelial cell lines by DNA synthesis assays. Secretion of TGFβs by trophoblastic and endometrial epithelial cells was determined using bioassays. All forms of TGFβ were immunolocalized in bovine placentomes. TGFβ mRNA was expressed in chorioallantois, caruncles, and in cultured trophoblastic and endometrial epithelial cells. Endometrial and trophoblastic cells secreted active and latent TGFβs, and these cells had a transient proliferative response to all forms of TGFβ. These results indicate that TGFβs are present at the fetal-maternal interface of the bovine placentome and may promote endometrial and chorionic growth.
AB - Transforming growth factor βs (TGFβ) are a family of multifunctional growth factors that are important embryonic morphogens. Because TGFβs may regulate the development of epitheliochorial placentas, we investigated the location, expression, secretion, and effects of TGFβs in bovine placentomes and cell cultures derived from chorionic and endometrial epithelia. Placentomes from early second-trimester pregnancies were examined by immunohistochemistry for TGFβ1, TGFβ2, and TGFβ3, and for TGFβ expression in Northern slot-blots. Effects of TGFβs were assessed in trophoblastic and endometrial epithelial cell lines by DNA synthesis assays. Secretion of TGFβs by trophoblastic and endometrial epithelial cells was determined using bioassays. All forms of TGFβ were immunolocalized in bovine placentomes. TGFβ mRNA was expressed in chorioallantois, caruncles, and in cultured trophoblastic and endometrial epithelial cells. Endometrial and trophoblastic cells secreted active and latent TGFβs, and these cells had a transient proliferative response to all forms of TGFβ. These results indicate that TGFβs are present at the fetal-maternal interface of the bovine placentome and may promote endometrial and chorionic growth.
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M3 - Article
C2 - 8879485
AN - SCOPUS:0029787507
VL - 55
SP - 748
EP - 755
JO - Biology of Reproduction
JF - Biology of Reproduction
SN - 0006-3363
IS - 4
ER -