Transformation of mammalian fibroblasts and macrophages in vitro by a murine retrovirus encoding an avian v-myc oncogene.

B. Vennström, P. Kahn, B. Adkins, P. Enrietto, M. J. Hayman, T. Graf, Paul A Luciw

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Abstract

A murine retrovirus which expresses the avain v-myc OK10 oncogene was constructed. The virus, denoted MMCV, readily transforms fibroblasts of established lines, such as mouse NIH/3T3 and rat 208F cells, to anchorage-independent growth in agarose. The virus also transforms primary mouse cells: (i) virus-infected macrophages are induced to form large colonies in semi-solid media, and can easily be expanded into mass cultures; (ii) MMCV-infected fibroblastic cells from mouse limb buds undergo morphological transformation and grow in semi-solid medium. MMCV thus transforms both mouse fibroblastic cells and macrophages in vitro, in a fashion similar to the v-myc-containing avian viruses in chicken cells. The possibility of introducing a transforming myc gene into mammalian cells by virus infection provides a novel approach for studying the mechanism of myc transformation in cells from many lineages.

Original languageEnglish (US)
Pages (from-to)3223-3229
Number of pages7
JournalEMBO Journal
Volume3
Issue number13
StatePublished - Dec 20 1984
Externally publishedYes

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ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Vennström, B., Kahn, P., Adkins, B., Enrietto, P., Hayman, M. J., Graf, T., & Luciw, P. A. (1984). Transformation of mammalian fibroblasts and macrophages in vitro by a murine retrovirus encoding an avian v-myc oncogene. EMBO Journal, 3(13), 3223-3229.