Transformation of lettuce (Lactuca sativa) mediated by Agrobacterium tumefaciens

Richard W Michelmore, Ellen Marsh, Susan Seely, Benoit Landry

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

Lactuca sativa can be routinely transformed using Ti plasmids of Agrobacterium tumefaciens containing a chimeric kanamycin resistance gene (NOS.NPTII.NOS). Critical experimental variables were plant genotype, bacterial concentration, presence of a nurse culture and timing of transfers between tissue culture media. Transformation was confirmed by the ability to callus and root in the presence of kanamycin, nopaline production, and by hybridization in Southern blots. Transformation has been achieved with several Ti vectors. Several hundred transformed plants have been regenerated. Kanamycin resistance was inherited monogenically. Homozygotes can be selected by growing R2 seedlings on media containing G418.

Original languageEnglish (US)
Pages (from-to)439-442
Number of pages4
JournalPlant Cell Reports
Volume6
Issue number6
DOIs
StatePublished - Dec 1987

ASJC Scopus subject areas

  • Plant Science

Fingerprint Dive into the research topics of 'Transformation of lettuce (Lactuca sativa) mediated by Agrobacterium tumefaciens'. Together they form a unique fingerprint.

  • Cite this