Transformation of both erythroid and myeloid cells by E26, an avian leukemia virus that contains the myb gene

Kathryn Radke, Hartmut Beug, Shaul Kornfeld, Thomas Graf

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

E26 and avian myeloblastosis virus are replication-defective avian retroviruses that contain the myb oncogene and cause leukemia in chickens with short periods of latency. Animals infected with E26 develop erythroleukemia and also contain low numbers of transformed myeloid cells, while avian myeloblastosis virus induces a purely myeloid leukemia. In both cases the type of leukemia induced is independent of the subgroup of the helper virus used. E26-transformed erythroid and myeloid cells can each be propagated selectively from explanted leukemic cells with media supplemented with factors that promote the growth either of normal chicken erythroid precursors or of myeloid progenitor cells. E26 also induces the outgrowth of transformed cells from bone marrow cells infected in vitro. These cells are also either erythroid or myeloid, depending on the culture conditions employed. Most of the erythroid cells transformed by E26 are erythroblast-like, but a significant number are more mature, including erythrocyte-like cells as well as some cells that appear to be aberrant in differentiation. Both erythroid and myeloid E26-transformed cells produce infectious virus and express P135 E26, the putative (gag -myb- x) transforming protein of the virus. Thus E26 is a virus that is capable of generating factor-dependent transformed cells in two different hematopoietic lineages.

Original languageEnglish (US)
Pages (from-to)643-653
Number of pages11
JournalCell
Volume31
Issue number3 PART 2
DOIs
StatePublished - 1982
Externally publishedYes

Fingerprint

myb Genes
Erythroid Cells
Myeloid Cells
Viruses
Leukemia
Genes
Avian Myeloblastosis Virus
Chickens
Helper Viruses
Myeloid Progenitor Cells
Erythroblasts
Leukemia, Erythroblastic, Acute
Myeloid Leukemia
Retroviridae
Virus Replication
Intercellular Signaling Peptides and Proteins
Bone
Bone Marrow Cells
Animals
Cells

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Transformation of both erythroid and myeloid cells by E26, an avian leukemia virus that contains the myb gene. / Radke, Kathryn; Beug, Hartmut; Kornfeld, Shaul; Graf, Thomas.

In: Cell, Vol. 31, No. 3 PART 2, 1982, p. 643-653.

Research output: Contribution to journalArticle

Radke, Kathryn ; Beug, Hartmut ; Kornfeld, Shaul ; Graf, Thomas. / Transformation of both erythroid and myeloid cells by E26, an avian leukemia virus that contains the myb gene. In: Cell. 1982 ; Vol. 31, No. 3 PART 2. pp. 643-653.
@article{e297cbff93b34730a5a60818d422ee15,
title = "Transformation of both erythroid and myeloid cells by E26, an avian leukemia virus that contains the myb gene",
abstract = "E26 and avian myeloblastosis virus are replication-defective avian retroviruses that contain the myb oncogene and cause leukemia in chickens with short periods of latency. Animals infected with E26 develop erythroleukemia and also contain low numbers of transformed myeloid cells, while avian myeloblastosis virus induces a purely myeloid leukemia. In both cases the type of leukemia induced is independent of the subgroup of the helper virus used. E26-transformed erythroid and myeloid cells can each be propagated selectively from explanted leukemic cells with media supplemented with factors that promote the growth either of normal chicken erythroid precursors or of myeloid progenitor cells. E26 also induces the outgrowth of transformed cells from bone marrow cells infected in vitro. These cells are also either erythroid or myeloid, depending on the culture conditions employed. Most of the erythroid cells transformed by E26 are erythroblast-like, but a significant number are more mature, including erythrocyte-like cells as well as some cells that appear to be aberrant in differentiation. Both erythroid and myeloid E26-transformed cells produce infectious virus and express P135 E26, the putative (gag -myb- x) transforming protein of the virus. Thus E26 is a virus that is capable of generating factor-dependent transformed cells in two different hematopoietic lineages.",
author = "Kathryn Radke and Hartmut Beug and Shaul Kornfeld and Thomas Graf",
year = "1982",
doi = "10.1016/0092-8674(82)90320-8",
language = "English (US)",
volume = "31",
pages = "643--653",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "3 PART 2",

}

TY - JOUR

T1 - Transformation of both erythroid and myeloid cells by E26, an avian leukemia virus that contains the myb gene

AU - Radke, Kathryn

AU - Beug, Hartmut

AU - Kornfeld, Shaul

AU - Graf, Thomas

PY - 1982

Y1 - 1982

N2 - E26 and avian myeloblastosis virus are replication-defective avian retroviruses that contain the myb oncogene and cause leukemia in chickens with short periods of latency. Animals infected with E26 develop erythroleukemia and also contain low numbers of transformed myeloid cells, while avian myeloblastosis virus induces a purely myeloid leukemia. In both cases the type of leukemia induced is independent of the subgroup of the helper virus used. E26-transformed erythroid and myeloid cells can each be propagated selectively from explanted leukemic cells with media supplemented with factors that promote the growth either of normal chicken erythroid precursors or of myeloid progenitor cells. E26 also induces the outgrowth of transformed cells from bone marrow cells infected in vitro. These cells are also either erythroid or myeloid, depending on the culture conditions employed. Most of the erythroid cells transformed by E26 are erythroblast-like, but a significant number are more mature, including erythrocyte-like cells as well as some cells that appear to be aberrant in differentiation. Both erythroid and myeloid E26-transformed cells produce infectious virus and express P135 E26, the putative (gag -myb- x) transforming protein of the virus. Thus E26 is a virus that is capable of generating factor-dependent transformed cells in two different hematopoietic lineages.

AB - E26 and avian myeloblastosis virus are replication-defective avian retroviruses that contain the myb oncogene and cause leukemia in chickens with short periods of latency. Animals infected with E26 develop erythroleukemia and also contain low numbers of transformed myeloid cells, while avian myeloblastosis virus induces a purely myeloid leukemia. In both cases the type of leukemia induced is independent of the subgroup of the helper virus used. E26-transformed erythroid and myeloid cells can each be propagated selectively from explanted leukemic cells with media supplemented with factors that promote the growth either of normal chicken erythroid precursors or of myeloid progenitor cells. E26 also induces the outgrowth of transformed cells from bone marrow cells infected in vitro. These cells are also either erythroid or myeloid, depending on the culture conditions employed. Most of the erythroid cells transformed by E26 are erythroblast-like, but a significant number are more mature, including erythrocyte-like cells as well as some cells that appear to be aberrant in differentiation. Both erythroid and myeloid E26-transformed cells produce infectious virus and express P135 E26, the putative (gag -myb- x) transforming protein of the virus. Thus E26 is a virus that is capable of generating factor-dependent transformed cells in two different hematopoietic lineages.

UR - http://www.scopus.com/inward/record.url?scp=0020458081&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020458081&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(82)90320-8

DO - 10.1016/0092-8674(82)90320-8

M3 - Article

VL - 31

SP - 643

EP - 653

JO - Cell

JF - Cell

SN - 0092-8674

IS - 3 PART 2

ER -