Transformation by Rous sarcoma virus: Effects of src-gene expression on the synthesis and phosphorylation of cellular polypeptides

K. Radke, G. S. Martin

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Citations (Scopus)

Abstract

To investigate the effects of transformation on the synthesis and phosphorylation of cellular polypeptides, we have used two-dimensional polyacrylamide gel electrophoresis to examine the radioactively labeled polypeptides of chick embryo fibroblasts infected either with nondefective (nd) RSV or with transformation-defective (td) or temperature-sensitive mutants. Infection by RSV does not appear to induce the de novo synthesis or the complete suppression of any of the [35S]methionine-labeled cellular polypeptides that can be resolved with this technique; however, there are quantitative changes in a minor fraction (approximately 4%) of the [35S]methionine-labeled polypeptides. When cells labeled with [32P]orthophosphate were examined, a phosphorylated polypeptide (M(r)=36,000) was detected in transformed cells, in addition to a poorly resolved streak of spots (M(r)=60,000), which may represent the product of the src gene (pp60(src)). the 36,000-M(r) phosphorylated polypeptide appears within 20 minutes when cells infected by a temperature-sensitive mutant of RSV are shifted from the nonpermissive to the permissive temperature; this change occurs even when protein synthesis is inhibited by cycloheximide. Phosphorylation of the 36,000-M(r) polypeptide thus represents an early event in the process of transformation, and it is possible that this polypeptide is a target for the kinase activity associated with pp60(src).

Original languageEnglish (US)
Title of host publicationSymposia on Quantitative Biology
Pages975-982
Number of pages8
Volume44
Edition2
StatePublished - 1979

Fingerprint

src Genes
Rous sarcoma virus
Phosphorylation
Gene Expression
Peptides
Methionine
Temperature
Electrophoresis, Gel, Two-Dimensional
Chick Embryo
Cycloheximide
Phosphotransferases
Fibroblasts
Phosphates

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Radke, K., & Martin, G. S. (1979). Transformation by Rous sarcoma virus: Effects of src-gene expression on the synthesis and phosphorylation of cellular polypeptides. In Symposia on Quantitative Biology (2 ed., Vol. 44, pp. 975-982)

Transformation by Rous sarcoma virus : Effects of src-gene expression on the synthesis and phosphorylation of cellular polypeptides. / Radke, K.; Martin, G. S.

Symposia on Quantitative Biology. Vol. 44 2. ed. 1979. p. 975-982.

Research output: Chapter in Book/Report/Conference proceedingChapter

Radke, K & Martin, GS 1979, Transformation by Rous sarcoma virus: Effects of src-gene expression on the synthesis and phosphorylation of cellular polypeptides. in Symposia on Quantitative Biology. 2 edn, vol. 44, pp. 975-982.
Radke K, Martin GS. Transformation by Rous sarcoma virus: Effects of src-gene expression on the synthesis and phosphorylation of cellular polypeptides. In Symposia on Quantitative Biology. 2 ed. Vol. 44. 1979. p. 975-982
Radke, K. ; Martin, G. S. / Transformation by Rous sarcoma virus : Effects of src-gene expression on the synthesis and phosphorylation of cellular polypeptides. Symposia on Quantitative Biology. Vol. 44 2. ed. 1979. pp. 975-982
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