Abstract
To investigate the effects of transformation on the synthesis and phosphorylation of cellular polypeptides, we have used two-dimensional polyacrylamide gel electrophoresis to examine the radioactively labeled polypeptides of chick embryo fibroblasts infected either with nondefective (nd) RSV or with transformation-defective (td) or temperature-sensitive mutants. Infection by RSV does not appear to induce the de novo synthesis or the complete suppression of any of the [35S]methionine-labeled cellular polypeptides that can be resolved with this technique; however, there are quantitative changes in a minor fraction (approximately 4%) of the [35S]methionine-labeled polypeptides. When cells labeled with [32P]orthophosphate were examined, a phosphorylated polypeptide (M(r)=36,000) was detected in transformed cells, in addition to a poorly resolved streak of spots (M(r)=60,000), which may represent the product of the src gene (pp60(src)). the 36,000-M(r) phosphorylated polypeptide appears within 20 minutes when cells infected by a temperature-sensitive mutant of RSV are shifted from the nonpermissive to the permissive temperature; this change occurs even when protein synthesis is inhibited by cycloheximide. Phosphorylation of the 36,000-M(r) polypeptide thus represents an early event in the process of transformation, and it is possible that this polypeptide is a target for the kinase activity associated with pp60(src).
| Original language | English (US) |
|---|---|
| Title of host publication | Symposia on Quantitative Biology |
| Pages | 975-982 |
| Number of pages | 8 |
| Volume | 44 |
| Edition | 2 |
| State | Published - 1979 |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
Transformation by Rous sarcoma virus : Effects of src-gene expression on the synthesis and phosphorylation of cellular polypeptides. / Radke, K.; Martin, G. S.
Symposia on Quantitative Biology. Vol. 44 2. ed. 1979. p. 975-982.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Transformation by Rous sarcoma virus
T2 - Effects of src-gene expression on the synthesis and phosphorylation of cellular polypeptides
AU - Radke, K.
AU - Martin, G. S.
PY - 1979
Y1 - 1979
N2 - To investigate the effects of transformation on the synthesis and phosphorylation of cellular polypeptides, we have used two-dimensional polyacrylamide gel electrophoresis to examine the radioactively labeled polypeptides of chick embryo fibroblasts infected either with nondefective (nd) RSV or with transformation-defective (td) or temperature-sensitive mutants. Infection by RSV does not appear to induce the de novo synthesis or the complete suppression of any of the [35S]methionine-labeled cellular polypeptides that can be resolved with this technique; however, there are quantitative changes in a minor fraction (approximately 4%) of the [35S]methionine-labeled polypeptides. When cells labeled with [32P]orthophosphate were examined, a phosphorylated polypeptide (M(r)=36,000) was detected in transformed cells, in addition to a poorly resolved streak of spots (M(r)=60,000), which may represent the product of the src gene (pp60(src)). the 36,000-M(r) phosphorylated polypeptide appears within 20 minutes when cells infected by a temperature-sensitive mutant of RSV are shifted from the nonpermissive to the permissive temperature; this change occurs even when protein synthesis is inhibited by cycloheximide. Phosphorylation of the 36,000-M(r) polypeptide thus represents an early event in the process of transformation, and it is possible that this polypeptide is a target for the kinase activity associated with pp60(src).
AB - To investigate the effects of transformation on the synthesis and phosphorylation of cellular polypeptides, we have used two-dimensional polyacrylamide gel electrophoresis to examine the radioactively labeled polypeptides of chick embryo fibroblasts infected either with nondefective (nd) RSV or with transformation-defective (td) or temperature-sensitive mutants. Infection by RSV does not appear to induce the de novo synthesis or the complete suppression of any of the [35S]methionine-labeled cellular polypeptides that can be resolved with this technique; however, there are quantitative changes in a minor fraction (approximately 4%) of the [35S]methionine-labeled polypeptides. When cells labeled with [32P]orthophosphate were examined, a phosphorylated polypeptide (M(r)=36,000) was detected in transformed cells, in addition to a poorly resolved streak of spots (M(r)=60,000), which may represent the product of the src gene (pp60(src)). the 36,000-M(r) phosphorylated polypeptide appears within 20 minutes when cells infected by a temperature-sensitive mutant of RSV are shifted from the nonpermissive to the permissive temperature; this change occurs even when protein synthesis is inhibited by cycloheximide. Phosphorylation of the 36,000-M(r) polypeptide thus represents an early event in the process of transformation, and it is possible that this polypeptide is a target for the kinase activity associated with pp60(src).
UR - http://www.scopus.com/inward/record.url?scp=0018711705&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018711705&partnerID=8YFLogxK
M3 - Chapter
C2 - 6253229
AN - SCOPUS:0018711705
VL - 44
SP - 975
EP - 982
BT - Symposia on Quantitative Biology
ER -