Transfer of cultured bovine embryos

D. F. Peters, G. B. Anderson, Robert Bondurant, P. T. Cupps, M. Drost

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A total of 126 bovine embryos were surgically collected from 16 superovulated donor heifers 5 days after estrus and randomly selected for either immediate transfer to synchronized recipients or in vitro culture at 37°C for 24 hours and subsequent transfer. Twenty-four of 56 (42.8%) embryos maintained for 24 hours in Ham's F10 medium supplemented with 10% heat treated fetal calf serum (HTFCS) and transferred to 32 recipients produced live calves. Survival of 70 noncultured embryos transferred to 35 recipients was 55.7% (39 calves). The percentages of recipients that were diagnosed pregnant at 42 days with cultured and control embryos were 59.4% ( 19 32) and 74.3% ( 26 35), respectively. No statistical difference was observed between the in vitro cultured and control embryos for viability following transfer to recipient females. In a second study, Day 7 embryos maintained in Ham's F10 medium supplemented with 10% HTFC serum for various culture periods were tested for viability following nonsurgical transfer to recipient females. A total of 1 5, 1 3 and 0 4 embryos cultured for 24, 48 and 72 hours, respectively, resulted in pregnant recipients following transfer.

Original languageEnglish (US)
Pages (from-to)337-341
Number of pages5
JournalTheriogenology
Volume10
Issue number4
DOIs
StatePublished - Jan 1 1978

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

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    Peters, D. F., Anderson, G. B., Bondurant, R., Cupps, P. T., & Drost, M. (1978). Transfer of cultured bovine embryos. Theriogenology, 10(4), 337-341. https://doi.org/10.1016/0093-691X(78)90111-5