Transfer of cultured bovine embryos

D. F. Peters, G. B. Anderson, Robert Bondurant, P. T. Cupps, M. Drost

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A total of 126 bovine embryos were surgically collected from 16 superovulated donor heifers 5 days after estrus and randomly selected for either immediate transfer to synchronized recipients or in vitro culture at 37°C for 24 hours and subsequent transfer. Twenty-four of 56 (42.8%) embryos maintained for 24 hours in Ham's F10 medium supplemented with 10% heat treated fetal calf serum (HTFCS) and transferred to 32 recipients produced live calves. Survival of 70 noncultured embryos transferred to 35 recipients was 55.7% (39 calves). The percentages of recipients that were diagnosed pregnant at 42 days with cultured and control embryos were 59.4% ( 19 32) and 74.3% ( 26 35), respectively. No statistical difference was observed between the in vitro cultured and control embryos for viability following transfer to recipient females. In a second study, Day 7 embryos maintained in Ham's F10 medium supplemented with 10% HTFC serum for various culture periods were tested for viability following nonsurgical transfer to recipient females. A total of 1 5, 1 3 and 0 4 embryos cultured for 24, 48 and 72 hours, respectively, resulted in pregnant recipients following transfer.

Original languageEnglish (US)
Pages (from-to)337-341
Number of pages5
JournalTheriogenology
Volume10
Issue number4
DOIs
StatePublished - Jan 1 1978

Fingerprint

embryo (animal)
Embryonic Structures
cattle
ham
viability
calves
fetal bovine serum
Estrus
blood serum
Serum
in vitro culture
estrus
heifers
Hot Temperature
heat
In Vitro Techniques

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

Cite this

Peters, D. F., Anderson, G. B., Bondurant, R., Cupps, P. T., & Drost, M. (1978). Transfer of cultured bovine embryos. Theriogenology, 10(4), 337-341. https://doi.org/10.1016/0093-691X(78)90111-5

Transfer of cultured bovine embryos. / Peters, D. F.; Anderson, G. B.; Bondurant, Robert; Cupps, P. T.; Drost, M.

In: Theriogenology, Vol. 10, No. 4, 01.01.1978, p. 337-341.

Research output: Contribution to journalArticle

Peters, DF, Anderson, GB, Bondurant, R, Cupps, PT & Drost, M 1978, 'Transfer of cultured bovine embryos', Theriogenology, vol. 10, no. 4, pp. 337-341. https://doi.org/10.1016/0093-691X(78)90111-5
Peters DF, Anderson GB, Bondurant R, Cupps PT, Drost M. Transfer of cultured bovine embryos. Theriogenology. 1978 Jan 1;10(4):337-341. https://doi.org/10.1016/0093-691X(78)90111-5
Peters, D. F. ; Anderson, G. B. ; Bondurant, Robert ; Cupps, P. T. ; Drost, M. / Transfer of cultured bovine embryos. In: Theriogenology. 1978 ; Vol. 10, No. 4. pp. 337-341.
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