Abstract
A total of 126 bovine embryos were surgically collected from 16 superovulated donor heifers 5 days after estrus and randomly selected for either immediate transfer to synchronized recipients or in vitro culture at 37°C for 24 hours and subsequent transfer. Twenty-four of 56 (42.8%) embryos maintained for 24 hours in Ham's F10 medium supplemented with 10% heat treated fetal calf serum (HTFCS) and transferred to 32 recipients produced live calves. Survival of 70 noncultured embryos transferred to 35 recipients was 55.7% (39 calves). The percentages of recipients that were diagnosed pregnant at 42 days with cultured and control embryos were 59.4% ( 19 32) and 74.3% ( 26 35), respectively. No statistical difference was observed between the in vitro cultured and control embryos for viability following transfer to recipient females. In a second study, Day 7 embryos maintained in Ham's F10 medium supplemented with 10% HTFC serum for various culture periods were tested for viability following nonsurgical transfer to recipient females. A total of 1 5, 1 3 and 0 4 embryos cultured for 24, 48 and 72 hours, respectively, resulted in pregnant recipients following transfer.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 337-341 |
| Number of pages | 5 |
| Journal | Theriogenology |
| Volume | 10 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jan 1 1978 |
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ASJC Scopus subject areas
- Small Animals
- Food Animals
- Animal Science and Zoology
- Equine
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Transfer of cultured bovine embryos. / Peters, D. F.; Anderson, G. B.; Bondurant, Robert; Cupps, P. T.; Drost, M.
In: Theriogenology, Vol. 10, No. 4, 01.01.1978, p. 337-341.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transfer of cultured bovine embryos
AU - Peters, D. F.
AU - Anderson, G. B.
AU - Bondurant, Robert
AU - Cupps, P. T.
AU - Drost, M.
PY - 1978/1/1
Y1 - 1978/1/1
N2 - A total of 126 bovine embryos were surgically collected from 16 superovulated donor heifers 5 days after estrus and randomly selected for either immediate transfer to synchronized recipients or in vitro culture at 37°C for 24 hours and subsequent transfer. Twenty-four of 56 (42.8%) embryos maintained for 24 hours in Ham's F10 medium supplemented with 10% heat treated fetal calf serum (HTFCS) and transferred to 32 recipients produced live calves. Survival of 70 noncultured embryos transferred to 35 recipients was 55.7% (39 calves). The percentages of recipients that were diagnosed pregnant at 42 days with cultured and control embryos were 59.4% ( 19 32) and 74.3% ( 26 35), respectively. No statistical difference was observed between the in vitro cultured and control embryos for viability following transfer to recipient females. In a second study, Day 7 embryos maintained in Ham's F10 medium supplemented with 10% HTFC serum for various culture periods were tested for viability following nonsurgical transfer to recipient females. A total of 1 5, 1 3 and 0 4 embryos cultured for 24, 48 and 72 hours, respectively, resulted in pregnant recipients following transfer.
AB - A total of 126 bovine embryos were surgically collected from 16 superovulated donor heifers 5 days after estrus and randomly selected for either immediate transfer to synchronized recipients or in vitro culture at 37°C for 24 hours and subsequent transfer. Twenty-four of 56 (42.8%) embryos maintained for 24 hours in Ham's F10 medium supplemented with 10% heat treated fetal calf serum (HTFCS) and transferred to 32 recipients produced live calves. Survival of 70 noncultured embryos transferred to 35 recipients was 55.7% (39 calves). The percentages of recipients that were diagnosed pregnant at 42 days with cultured and control embryos were 59.4% ( 19 32) and 74.3% ( 26 35), respectively. No statistical difference was observed between the in vitro cultured and control embryos for viability following transfer to recipient females. In a second study, Day 7 embryos maintained in Ham's F10 medium supplemented with 10% HTFC serum for various culture periods were tested for viability following nonsurgical transfer to recipient females. A total of 1 5, 1 3 and 0 4 embryos cultured for 24, 48 and 72 hours, respectively, resulted in pregnant recipients following transfer.
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U2 - 10.1016/0093-691X(78)90111-5
DO - 10.1016/0093-691X(78)90111-5
M3 - Article
VL - 10
SP - 337
EP - 341
JO - Theriogenology
JF - Theriogenology
SN - 0093-691X
IS - 4
ER -