Transfection of a vector expressing wild-type p53 into cells of two human glioma cell lines enhances radiation toxicity

L. Geng, S. Walter, E. Melian, Andrew T M Vaughan

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Replication-deficient adenovirus (Adv5)-based vectors containing either wild-type p53 or the β-gal marker gene were introduced into cells of the T98G (p53 mutant) and U87MG (p53 wild-type) human glioma cell lines. The wild-type p53 gene was successfully expressed in each cell line as shown by flow cytometry and Western blotting. The presence of the p53-expressing vector was toxic in both cell lines compared to control cells or to those containing the β-gal vector. At levels of Adv5p53 vector that produced detectable toxicity, the effect of irradiation was enhanced, producing a twofold increase in cell killing. In the T98G cells, the presence of the p53 vector resulted in an increase in the number of cells undergoing apoptosis after irradiation, whereas a smaller and only additive response was observed in the U87MG cells. Conversely, an increase in micronucleus formation, indicating corrupt mitotic activity, was observed in irradiated Adv5p53- positive U87MG cells but not in T98G cells. These data suggest that p53- expressing vectors effectively enhance radiation lethality in these human glioma cell lines, but that the mechanism of action cannot be simply related to activation of the p53-dependent pathway to apoptosis.

Original languageEnglish (US)
Pages (from-to)31-37
Number of pages7
JournalRadiation Research
Volume150
Issue number1
DOIs
StatePublished - Jul 1998
Externally publishedYes

Fingerprint

transfection
cultured cells
Glioma
toxicity
Transfection
cell lines
Radiation
Cell Line
radiation
cells
apoptosis
genes
adenoviruses
Apoptosis
irradiation
lethality
cytometry
Poisons
p53 Genes
Adenoviridae

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Transfection of a vector expressing wild-type p53 into cells of two human glioma cell lines enhances radiation toxicity. / Geng, L.; Walter, S.; Melian, E.; Vaughan, Andrew T M.

In: Radiation Research, Vol. 150, No. 1, 07.1998, p. 31-37.

Research output: Contribution to journalArticle

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