Transcriptome analysis of salmonella heidelberg after exposure to cetylpyridinium chloride, acidified calcium hypochlorite, and peroxyacetic acid

Myrna Cadena, Lutz Froenicke, Monica Britton, Matthew L. Settles, Blythe Durbin-Johnson, Emily Kumimoto, Rodrigo A Gallardo, Aura Ferreiro, Tereza Chylkova, Huaijun Zhou, Maurice Pitesky

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The application of RNA sequencing in commercial poultry could facilitate a novel approach toward food safety with respect to identifying conditions in food production that mitigate transcription of genes associated with virulence and survivability. In this study, we evaluated the effects of disinfectant exposure on the transcriptomes of two field isolates of Salmonella Heidelberg (SH) isolated from a commercial broiler processing plant in 1992 and 2014. The isolates were each exposed separately to the following disinfectants commonly used in poultry processing: cetylpyridinium chloride (CPC), acidified calcium hypochlorite (aCH), and peroxyacetic acid (PAA). Exposure times were 8 s with CPC to simulate a poultry processing dipping station or 90 min with aCH and PAA to simulate the chiller tank in a poultry processing plant at 4°C. Based on comparison with a publicly available annotated SH reference genome with 5,088 genes, 90 genes were identified as associated with virulence, pathogenicity, and resistance (VPR). Of these 90 VPR genes, 9 (10.0%), 28 (31.1%), and 1 (1.1%) gene were upregulated in SH 2014 and 21 (23.3%), 26 (28.9%), and 2 (2.2%) genes were upregulated in SH 2014 challenged with CPC, aCH, and PAA, respectively. This information and previously reported MICs for the three disinfectants with both SH isolates allow researchers to make more accurate recommendations regarding control methods of SH and public health considerations related to SH in food production facilities where SH has been isolated. For example, the MICs revealed that aCH is ineffective for SH inhibition at regulatory levels allowed for poultry processing and that aCH was ineffective for inhibiting SH growth and caused an upregulation of VPR genes.

Original languageEnglish (US)
Pages (from-to)109-119
Number of pages11
JournalJournal of Food Protection
Volume82
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

calcium hypochlorite
Salmonella Heidelberg
cetylpyridinium chloride
Cetylpyridinium
Peracetic Acid
peracetic acid
Gene Expression Profiling
transcriptomics
Salmonella
Virulence
Poultry
Disinfectants
disinfectants
poultry
Genes
virulence
genes
meat processing plants
pathogenicity
food production

Keywords

  • Differential gene expression
  • Disinfectant
  • Poultry processing
  • RNA sequencing
  • Salmonella

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Transcriptome analysis of salmonella heidelberg after exposure to cetylpyridinium chloride, acidified calcium hypochlorite, and peroxyacetic acid. / Cadena, Myrna; Froenicke, Lutz; Britton, Monica; Settles, Matthew L.; Durbin-Johnson, Blythe; Kumimoto, Emily; Gallardo, Rodrigo A; Ferreiro, Aura; Chylkova, Tereza; Zhou, Huaijun; Pitesky, Maurice.

In: Journal of Food Protection, Vol. 82, No. 1, 01.01.2019, p. 109-119.

Research output: Contribution to journalArticle

Cadena, M, Froenicke, L, Britton, M, Settles, ML, Durbin-Johnson, B, Kumimoto, E, Gallardo, RA, Ferreiro, A, Chylkova, T, Zhou, H & Pitesky, M 2019, 'Transcriptome analysis of salmonella heidelberg after exposure to cetylpyridinium chloride, acidified calcium hypochlorite, and peroxyacetic acid', Journal of Food Protection, vol. 82, no. 1, pp. 109-119. https://doi.org/10.4315/0362-028X.JFP-18-235
Cadena, Myrna ; Froenicke, Lutz ; Britton, Monica ; Settles, Matthew L. ; Durbin-Johnson, Blythe ; Kumimoto, Emily ; Gallardo, Rodrigo A ; Ferreiro, Aura ; Chylkova, Tereza ; Zhou, Huaijun ; Pitesky, Maurice. / Transcriptome analysis of salmonella heidelberg after exposure to cetylpyridinium chloride, acidified calcium hypochlorite, and peroxyacetic acid. In: Journal of Food Protection. 2019 ; Vol. 82, No. 1. pp. 109-119.
@article{31da5a722b574c579a5ae160fd021546,
title = "Transcriptome analysis of salmonella heidelberg after exposure to cetylpyridinium chloride, acidified calcium hypochlorite, and peroxyacetic acid",
abstract = "The application of RNA sequencing in commercial poultry could facilitate a novel approach toward food safety with respect to identifying conditions in food production that mitigate transcription of genes associated with virulence and survivability. In this study, we evaluated the effects of disinfectant exposure on the transcriptomes of two field isolates of Salmonella Heidelberg (SH) isolated from a commercial broiler processing plant in 1992 and 2014. The isolates were each exposed separately to the following disinfectants commonly used in poultry processing: cetylpyridinium chloride (CPC), acidified calcium hypochlorite (aCH), and peroxyacetic acid (PAA). Exposure times were 8 s with CPC to simulate a poultry processing dipping station or 90 min with aCH and PAA to simulate the chiller tank in a poultry processing plant at 4°C. Based on comparison with a publicly available annotated SH reference genome with 5,088 genes, 90 genes were identified as associated with virulence, pathogenicity, and resistance (VPR). Of these 90 VPR genes, 9 (10.0{\%}), 28 (31.1{\%}), and 1 (1.1{\%}) gene were upregulated in SH 2014 and 21 (23.3{\%}), 26 (28.9{\%}), and 2 (2.2{\%}) genes were upregulated in SH 2014 challenged with CPC, aCH, and PAA, respectively. This information and previously reported MICs for the three disinfectants with both SH isolates allow researchers to make more accurate recommendations regarding control methods of SH and public health considerations related to SH in food production facilities where SH has been isolated. For example, the MICs revealed that aCH is ineffective for SH inhibition at regulatory levels allowed for poultry processing and that aCH was ineffective for inhibiting SH growth and caused an upregulation of VPR genes.",
keywords = "Differential gene expression, Disinfectant, Poultry processing, RNA sequencing, Salmonella",
author = "Myrna Cadena and Lutz Froenicke and Monica Britton and Settles, {Matthew L.} and Blythe Durbin-Johnson and Emily Kumimoto and Gallardo, {Rodrigo A} and Aura Ferreiro and Tereza Chylkova and Huaijun Zhou and Maurice Pitesky",
year = "2019",
month = "1",
day = "1",
doi = "10.4315/0362-028X.JFP-18-235",
language = "English (US)",
volume = "82",
pages = "109--119",
journal = "Journal of Food Protection",
issn = "0362-028X",
publisher = "International Association for Food Protection",
number = "1",

}

TY - JOUR

T1 - Transcriptome analysis of salmonella heidelberg after exposure to cetylpyridinium chloride, acidified calcium hypochlorite, and peroxyacetic acid

AU - Cadena, Myrna

AU - Froenicke, Lutz

AU - Britton, Monica

AU - Settles, Matthew L.

AU - Durbin-Johnson, Blythe

AU - Kumimoto, Emily

AU - Gallardo, Rodrigo A

AU - Ferreiro, Aura

AU - Chylkova, Tereza

AU - Zhou, Huaijun

AU - Pitesky, Maurice

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The application of RNA sequencing in commercial poultry could facilitate a novel approach toward food safety with respect to identifying conditions in food production that mitigate transcription of genes associated with virulence and survivability. In this study, we evaluated the effects of disinfectant exposure on the transcriptomes of two field isolates of Salmonella Heidelberg (SH) isolated from a commercial broiler processing plant in 1992 and 2014. The isolates were each exposed separately to the following disinfectants commonly used in poultry processing: cetylpyridinium chloride (CPC), acidified calcium hypochlorite (aCH), and peroxyacetic acid (PAA). Exposure times were 8 s with CPC to simulate a poultry processing dipping station or 90 min with aCH and PAA to simulate the chiller tank in a poultry processing plant at 4°C. Based on comparison with a publicly available annotated SH reference genome with 5,088 genes, 90 genes were identified as associated with virulence, pathogenicity, and resistance (VPR). Of these 90 VPR genes, 9 (10.0%), 28 (31.1%), and 1 (1.1%) gene were upregulated in SH 2014 and 21 (23.3%), 26 (28.9%), and 2 (2.2%) genes were upregulated in SH 2014 challenged with CPC, aCH, and PAA, respectively. This information and previously reported MICs for the three disinfectants with both SH isolates allow researchers to make more accurate recommendations regarding control methods of SH and public health considerations related to SH in food production facilities where SH has been isolated. For example, the MICs revealed that aCH is ineffective for SH inhibition at regulatory levels allowed for poultry processing and that aCH was ineffective for inhibiting SH growth and caused an upregulation of VPR genes.

AB - The application of RNA sequencing in commercial poultry could facilitate a novel approach toward food safety with respect to identifying conditions in food production that mitigate transcription of genes associated with virulence and survivability. In this study, we evaluated the effects of disinfectant exposure on the transcriptomes of two field isolates of Salmonella Heidelberg (SH) isolated from a commercial broiler processing plant in 1992 and 2014. The isolates were each exposed separately to the following disinfectants commonly used in poultry processing: cetylpyridinium chloride (CPC), acidified calcium hypochlorite (aCH), and peroxyacetic acid (PAA). Exposure times were 8 s with CPC to simulate a poultry processing dipping station or 90 min with aCH and PAA to simulate the chiller tank in a poultry processing plant at 4°C. Based on comparison with a publicly available annotated SH reference genome with 5,088 genes, 90 genes were identified as associated with virulence, pathogenicity, and resistance (VPR). Of these 90 VPR genes, 9 (10.0%), 28 (31.1%), and 1 (1.1%) gene were upregulated in SH 2014 and 21 (23.3%), 26 (28.9%), and 2 (2.2%) genes were upregulated in SH 2014 challenged with CPC, aCH, and PAA, respectively. This information and previously reported MICs for the three disinfectants with both SH isolates allow researchers to make more accurate recommendations regarding control methods of SH and public health considerations related to SH in food production facilities where SH has been isolated. For example, the MICs revealed that aCH is ineffective for SH inhibition at regulatory levels allowed for poultry processing and that aCH was ineffective for inhibiting SH growth and caused an upregulation of VPR genes.

KW - Differential gene expression

KW - Disinfectant

KW - Poultry processing

KW - RNA sequencing

KW - Salmonella

UR - http://www.scopus.com/inward/record.url?scp=85059528614&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85059528614&partnerID=8YFLogxK

U2 - 10.4315/0362-028X.JFP-18-235

DO - 10.4315/0362-028X.JFP-18-235

M3 - Article

C2 - 30702951

AN - SCOPUS:85059528614

VL - 82

SP - 109

EP - 119

JO - Journal of Food Protection

JF - Journal of Food Protection

SN - 0362-028X

IS - 1

ER -