Transcriptional response of lymphoblastoid cells to ionizing radiation

Kuang-Yu Jen, Vivian G. Cheung

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

The effects of ionizing radiation (IR) on the temporal transcriptional response of lymphoblastoid cells were investigated in this study. We used oligonucleotide microarrays to assess mRNA levels of genes in lymphoblastoid cells at various time points within 24 h following γ-irradiation. We identified 319 and 816 IR-responsive genes following 3 Gy and 10 Gy of IR exposure, respectively, with 126 genes in common between the two doses. A high percentage of IR-responsive genes are involved in the control of cell cycle, cell death, DNA repair, DNA metabolism, and RNA processing. We determined the temporal expression profiles of the IR-responsive genes and assessed effects of IR dose on this temporal pattern of expression. By combining dose-response data with temporal profiles of expression, we have identified sets of coordinately responding genes. Through a genomic approach, we characterized a set of genes that are implicated in cellular adaptation to IR stress. These findings will allow a better understanding of complex processes such as radiation-induced carcinogenesis and the development of biomarkers for radiation exposure.

Original languageEnglish (US)
Pages (from-to)2092-2100
Number of pages9
JournalGenome Research
Volume13
Issue number9
DOIs
StatePublished - Sep 1 2003
Externally publishedYes

Fingerprint

Ionizing Radiation
Genes
Radiation Dosage
Cell Cycle Checkpoints
Oligonucleotide Array Sequence Analysis
DNA Repair
Carcinogenesis
Cell Death
Biomarkers
RNA
Radiation
Messenger RNA
DNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Transcriptional response of lymphoblastoid cells to ionizing radiation. / Jen, Kuang-Yu; Cheung, Vivian G.

In: Genome Research, Vol. 13, No. 9, 01.09.2003, p. 2092-2100.

Research output: Contribution to journalArticle

Jen, Kuang-Yu ; Cheung, Vivian G. / Transcriptional response of lymphoblastoid cells to ionizing radiation. In: Genome Research. 2003 ; Vol. 13, No. 9. pp. 2092-2100.
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