Abstract
A variety of eukaryotic transcription factors, including the nuclear hormone receptors, Max-Mad, BCL-6, and PLZF, appear to mediate transcriptional repression through the ability to recruit a multiprotein corepressor complex to the target promoter. This corepressor complex includes the SMRT/N-CoR polypeptides, mSin3A or -B, and histone deacetylase 1 or 2. The presence of a histone-modifying activity in the corepressor complex has led to the suggestion that gene silencing is mediated by modification of the chromatin template, perhaps rendering it less accessible to the transcriptional machinery. We report here, however, that the corepressor complex actually appears to exhibit multiple mechanisms of transcriptional repression, only one of which corresponds with detectable recruitment of the histone deacetylase. We provide evidence instead of an alternative pathway of repression that may be mediated by direct physical interactions between components of the corepressor complex and the general transcription factor TFIIB.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5500-5510 |
| Number of pages | 11 |
| Journal | Molecular and Cellular Biology |
| Volume | 18 |
| Issue number | 9 |
| State | Published - 1998 |
Fingerprint
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cell Biology
Cite this
Transcriptional repression by the SMRT-mSin3 corepressor : Multiple interactions, multiple mechanisms, and a potential role for TFIIB. / Wong, Chi Wai; Privalsky, Martin L.
In: Molecular and Cellular Biology, Vol. 18, No. 9, 1998, p. 5500-5510.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transcriptional repression by the SMRT-mSin3 corepressor
T2 - Multiple interactions, multiple mechanisms, and a potential role for TFIIB
AU - Wong, Chi Wai
AU - Privalsky, Martin L.
PY - 1998
Y1 - 1998
N2 - A variety of eukaryotic transcription factors, including the nuclear hormone receptors, Max-Mad, BCL-6, and PLZF, appear to mediate transcriptional repression through the ability to recruit a multiprotein corepressor complex to the target promoter. This corepressor complex includes the SMRT/N-CoR polypeptides, mSin3A or -B, and histone deacetylase 1 or 2. The presence of a histone-modifying activity in the corepressor complex has led to the suggestion that gene silencing is mediated by modification of the chromatin template, perhaps rendering it less accessible to the transcriptional machinery. We report here, however, that the corepressor complex actually appears to exhibit multiple mechanisms of transcriptional repression, only one of which corresponds with detectable recruitment of the histone deacetylase. We provide evidence instead of an alternative pathway of repression that may be mediated by direct physical interactions between components of the corepressor complex and the general transcription factor TFIIB.
AB - A variety of eukaryotic transcription factors, including the nuclear hormone receptors, Max-Mad, BCL-6, and PLZF, appear to mediate transcriptional repression through the ability to recruit a multiprotein corepressor complex to the target promoter. This corepressor complex includes the SMRT/N-CoR polypeptides, mSin3A or -B, and histone deacetylase 1 or 2. The presence of a histone-modifying activity in the corepressor complex has led to the suggestion that gene silencing is mediated by modification of the chromatin template, perhaps rendering it less accessible to the transcriptional machinery. We report here, however, that the corepressor complex actually appears to exhibit multiple mechanisms of transcriptional repression, only one of which corresponds with detectable recruitment of the histone deacetylase. We provide evidence instead of an alternative pathway of repression that may be mediated by direct physical interactions between components of the corepressor complex and the general transcription factor TFIIB.
UR - http://www.scopus.com/inward/record.url?scp=0031839177&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031839177&partnerID=8YFLogxK
M3 - Article
C2 - 9710634
AN - SCOPUS:0031839177
VL - 18
SP - 5500
EP - 5510
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 9
ER -