Transcriptional regulation of the human soluble epoxide hydrolase gene EPHX2

Hiromasa Tanaka, Shizuo G. Kamita, Nicola M. Wolf, Todd R. Harris, Zhaoju Wu, Christophe Morisseau, Bruce D. Hammock

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Soluble epoxide hydrolase (sEH) is a multifunctional protein encoded by the EPHX2 gene. The biological functions and enzyme kinetics of sEH have been extensively investigated, however, little is known about its transcriptional regulation and mechanisms of tissue specific expression. Here, a luciferase gene based reporter assay was used to identify the minimal promoter and cis regulatory elements of EPHX2. The minimal promoter was identified as a GC-rich region between nts - 374 and + 28 with respect to the putative transcriptional start site. A reporter plasmid carrying this minimal promoter showed higher or similar activities in comparison to a reporter plasmid carrying nts - 5,974 to + 28 of EPHX2 in 9 human cell lines that were tested. Sp1 binding sites that are involved in augmenting the minimal promoter activity of EPHX2 were identified by nested deletion analysis, site-specific mutation, electrophoretic mobility shift assay, and chromatin immunoprecipitation assay.

Original languageEnglish (US)
Pages (from-to)17-27
Number of pages11
JournalBiochimica et Biophysica Acta - Gene Regulatory Mechanisms
Volume1779
Issue number1
DOIs
StatePublished - Jan 2008

Fingerprint

Epoxide Hydrolases
Assays
Plasmids
Genes
GC Rich Sequence
Chromatin Immunoprecipitation
Electrophoretic Mobility Shift Assay
Luciferases
Reporter Genes
Enzyme kinetics
Electrophoretic mobility
Binding Sites
Cell Line
Mutation
Chromatin
Enzymes
Cells
Tissue
Proteins

Keywords

  • EPHX2
  • Epoxide hydrolase
  • Promoter
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Tanaka, H., Kamita, S. G., Wolf, N. M., Harris, T. R., Wu, Z., Morisseau, C., & Hammock, B. D. (2008). Transcriptional regulation of the human soluble epoxide hydrolase gene EPHX2. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 1779(1), 17-27. https://doi.org/10.1016/j.bbagrm.2007.11.005

Transcriptional regulation of the human soluble epoxide hydrolase gene EPHX2. / Tanaka, Hiromasa; Kamita, Shizuo G.; Wolf, Nicola M.; Harris, Todd R.; Wu, Zhaoju; Morisseau, Christophe; Hammock, Bruce D.

In: Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, Vol. 1779, No. 1, 01.2008, p. 17-27.

Research output: Contribution to journalArticle

Tanaka, H, Kamita, SG, Wolf, NM, Harris, TR, Wu, Z, Morisseau, C & Hammock, BD 2008, 'Transcriptional regulation of the human soluble epoxide hydrolase gene EPHX2', Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, vol. 1779, no. 1, pp. 17-27. https://doi.org/10.1016/j.bbagrm.2007.11.005
Tanaka, Hiromasa ; Kamita, Shizuo G. ; Wolf, Nicola M. ; Harris, Todd R. ; Wu, Zhaoju ; Morisseau, Christophe ; Hammock, Bruce D. / Transcriptional regulation of the human soluble epoxide hydrolase gene EPHX2. In: Biochimica et Biophysica Acta - Gene Regulatory Mechanisms. 2008 ; Vol. 1779, No. 1. pp. 17-27.
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