Abstract
Platelet-derived growth factor is expressed as dimers of two homologous polypeptide chains, termed A and B, encoded by different genes. A and B chain mRNA levels in microvascular endothelial cells are increased by phorbol ester, thrombin, and transforming growth factor-β (TGF-β) and are reduced by agents that elevate cyclic AMP. In this report, we investigated the effects of these regulatory agents on A and B chain transcription rates. By nuclear run-on analysis, TGF-β stimulated transcription of both A and B chain genes. Thrombin and phorbol ester stimulated B chain transcription and had little or no detectable effect on A chain transcription. Pretreatment of cultures with 50 μM forskolin, a potent activator of adenylyl cyclase, completely blocked B chain transcription by thrombin and TGF-β, but did not inhibit A chain transcription induced by TGF-β. These results show that expression of platelet-derived growth factor mRNA involves both positive and negative transcriptional regulation and that there are differences in the transcriptional control of the A and B chain genes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 8470-8472 |
| Number of pages | 3 |
| Journal | Journal of Biological Chemistry |
| Volume | 263 |
| Issue number | 17 |
| State | Published - 1988 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology