Transcriptional regulation of mouse dihydrofolate reductase in the cell cycle

P. J. Farnham, R. T. Schimke

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

We have studied transcription of the dihydrofolate reductase gene as cells progress through the cell cycle. We used the techniques of DNA-excess filter hybridization and in vivo pulse labeling of a synchronous population of cells to measure the transcription rate of the dihydrofolate reductase gene in different phases of the cell cycle. Our results indicate that the dihydrofolate reductase gene is cell cycle regulated at the transcriptional level. The transcription rate is low in G1, increases 7-fold at the beginning of S phase, decreases almost immediately thereafter, and remains low throughout the remainder of S and into G2. This cell cycle regulation seen in the G1 to S phase transition is achieved by increasing the rate of transcription from a single promoter region. The fact that this region is similar to promoter regions of other housekeeping genes suggests that this type of regulation may not be unique to dihydrofolate reductase.

Original languageEnglish (US)
Pages (from-to)7675-7680
Number of pages6
JournalJournal of Biological Chemistry
Volume260
Issue number12
StatePublished - 1985
Externally publishedYes

Fingerprint

Tetrahydrofolate Dehydrogenase
Transcription
Cell Cycle
Cells
Genes
S Phase
Genetic Promoter Regions
cdc Genes
Phase Transition
Essential Genes
Labeling
Phase transitions
DNA
Population

ASJC Scopus subject areas

  • Biochemistry

Cite this

Transcriptional regulation of mouse dihydrofolate reductase in the cell cycle. / Farnham, P. J.; Schimke, R. T.

In: Journal of Biological Chemistry, Vol. 260, No. 12, 1985, p. 7675-7680.

Research output: Contribution to journalArticle

Farnham, P. J. ; Schimke, R. T. / Transcriptional regulation of mouse dihydrofolate reductase in the cell cycle. In: Journal of Biological Chemistry. 1985 ; Vol. 260, No. 12. pp. 7675-7680.
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